BrdU Staining of Cells for Cell Cycle Analysis and Apoptosis for Flow Cytometry
BrdU is an analogue of thymidine readily incorporated into DNA during DNA synthesis and is an accurate method to monitor proliferation and apoptosis. The anti-BrdU antibody mouse monoclonal clone Bu20a (MCA2483) is suitable for flow cytometry. The following methods were used and provide a useful guide for using anti-BrdU antibodies.
The acid treatment to unwind the DNA may affect surface immunophenotyping. Staining of cells with BrdU using DNAseI may be applicable if this is required.
Appropriate controls should always be carried out, for flow cytometry the following should be considered for inclusion;
For all multi-color flow cytometry experiments it is advisable to include compensation controls and Fluorescence Minus One (FMO) controls, which assist with identifying gating boundaries.
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