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Protocol: PK Bridging ELISA for Use with Anti-Abatacept Antibodies
Pharmacokinetic (PK) Bridging ELISA: for Use with Mouse Anti-Human CTLA-4 (CD152) Antibody MCA1724 and Human Anti-Abatacept Antibody HCA335
Abatacept is a recombinant, fusion protein drug consisting of the extracellular domain of human cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4, also known as CD152), linked to a modified Fc portion of human immunoglobulin G1 (hIgG1). This method provides a procedure for carrying out a PK ELISA with Mouse Anti-Human CTLA-4 (CD152) Antibody, catalog number MCA1724 (capture antibody), and Human Anti-Abatacept Antibody, HCA335 (detection antibody), and using abatacept for the standard curve. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.