Hispec Assay Diluent | N/A

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  • Hispec Assay Diluent
  • Hispec Assay Diluent
  • Hispec Assay Diluent
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
  • Clone
    N/A
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF049BIYdatasheet pdfdatasheet pdf125 ml
    BUF049B
    BUF049AIYdatasheet pdfdatasheet pdf50 ml
    BUF049A
    BUF049CIYdatasheet pdfdatasheet pdf500 ml
    BUF049C
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
      HISPEC assay diluent works to reduce cross reactivity, non-specific binding and matrix effects in immunoassays such as ELISA, EIA, Western blotting, immuno-PCR, protein arrays, multianalyte immunoassays and immunohistochemistry.

      The effect of HISPEC assay diluent is dependent on assay system and the antibodies used. HISPEC assay diluent is used instead of a sample buffer or antibody dilution buffer within the immunoassay protocol.

      HISPEC assay diluent is unsuitable for blocking of surfaces. For blocking of surfaces we recommend one of the blocking solutions within the Bio-Rad range – see “Useful reagents” below.

      Examples of use:

      ELISA: use as a dilution buffer for the specimen and for the detection antibodies
      Western blotting: use as dilution buffer for primary and secondary antibodies
      Immunohistochemistry: use as dilution buffer for primary and secondary antibodies
      Protein arrays: use as dilution buffer for specimen and for the detection antibodies
      HISPEC assay diluent works to reduce cross reactivity, non-specific binding and matrix effects in immunoassays such as ELISA, EIA, Western blotting, immuno-PCR, protein arrays, multianalyte immunoassays and immunohistochemistry.

      The effect of HISPEC assay diluent is dependent on assay system and the antibodies used. HISPEC assay diluent is used instead of a sample buffer or antibody dilution buffer within the immunoassay protocol.

      HISPEC assay diluent is unsuitable for blocking of surfaces. For blocking of surfaces we recommend one of the blocking solutions within the Bio-Rad range – see “Useful reagents” below.

      Examples of use:

      ELISA: use as a dilution buffer for the specimen and for the detection antibodies
      Western blotting: use as dilution buffer for primary and secondary antibodies
      Immunohistochemistry: use as dilution buffer for primary and secondary antibodies
      Protein arrays: use as dilution buffer for specimen and for the detection antibodies
      HISPEC assay diluent works to reduce cross reactivity, non-specific binding and matrix effects in immunoassays such as ELISA, EIA, Western blotting, immuno-PCR, protein arrays, multianalyte immunoassays and immunohistochemistry.

      The effect of HISPEC assay diluent is dependent on assay system and the antibodies used. HISPEC assay diluent is used instead of a sample buffer or antibody dilution buffer within the immunoassay protocol.

      HISPEC assay diluent is unsuitable for blocking of surfaces. For blocking of surfaces we recommend one of the blocking solutions within the Bio-Rad range – see “Useful reagents” below.

      Examples of use:

      ELISA: use as a dilution buffer for the specimen and for the detection antibodies
      Western blotting: use as dilution buffer for primary and secondary antibodies
      Immunohistochemistry: use as dilution buffer for primary and secondary antibodies
      Protein arrays: use as dilution buffer for specimen and for the detection antibodies
    • Product Form
      Ready to use assay diluent buffer
      Ready to use assay diluent buffer
      Ready to use assay diluent buffer
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Proclin™ 300
      0.1% Proclin™ 300
      0.1% Proclin™ 300
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch at -20oC or 6 months at 2-8oC.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay
    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay
    • Application NameYesNoMin DilutionMax Dilution
      Immunoassay

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      The buffer should be mixed thoroughly immediately before use.
      Dilution of the samples:
      The standards and samples for ELISA and protein arrays can be diluted with HISPEC assay diluent at 1:2 or higher. Standards and samples should be treated identically.

      Dilution of antibodies:
      Antibodies can be diluted with HISPEC assay diluent in a user-defined manner, depending on the recommendation of the antibody supplier.

      Expected results:
      In some cases a reduction in assay signal intensity may be observed when using HISPEC assay diluent. This does not result in a reduction in assay sensitivity due to the accompanying reduction in non-specific binding.

      Signal intensity may be increased in some assay systems by use of higher concentrations of antibodies, or by dilution of the HISPEC assay diluent in distilled water (1:2) prior to use. However, these adjustments may also result in some associated increase in non-specific binding.
    • Instructions For Use
      The buffer should be mixed thoroughly immediately before use.
      Dilution of the samples:
      The standards and samples for ELISA and protein arrays can be diluted with HISPEC assay diluent at 1:2 or higher. Standards and samples should be treated identically.

      Dilution of antibodies:
      Antibodies can be diluted with HISPEC assay diluent in a user-defined manner, depending on the recommendation of the antibody supplier.

      Expected results:
      In some cases a reduction in assay signal intensity may be observed when using HISPEC assay diluent. This does not result in a reduction in assay sensitivity due to the accompanying reduction in non-specific binding.

      Signal intensity may be increased in some assay systems by use of higher concentrations of antibodies, or by dilution of the HISPEC assay diluent in distilled water (1:2) prior to use. However, these adjustments may also result in some associated increase in non-specific binding.
    • Instructions For Use
      The buffer should be mixed thoroughly immediately before use.
      Dilution of the samples:
      The standards and samples for ELISA and protein arrays can be diluted with HISPEC assay diluent at 1:2 or higher. Standards and samples should be treated identically.

      Dilution of antibodies:
      Antibodies can be diluted with HISPEC assay diluent in a user-defined manner, depending on the recommendation of the antibody supplier.

      Expected results:
      In some cases a reduction in assay signal intensity may be observed when using HISPEC assay diluent. This does not result in a reduction in assay sensitivity due to the accompanying reduction in non-specific binding.

      Signal intensity may be increased in some assay systems by use of higher concentrations of antibodies, or by dilution of the HISPEC assay diluent in distilled water (1:2) prior to use. However, these adjustments may also result in some associated increase in non-specific binding.

    Additional Hispec Assay Diluent Formats

    Formats Clone Applications Sizes available
    Hispec Assay Diluent : Ready To Use N/A IY 500 ml | 50 ml | 125 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          ELISA UltrablockBUF033A100 mlE
          BUF033A
          ELISA SynblockBUF034A100 mlE
          BUF034A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          ELISA UltrablockBUF033A100 mlE
          BUF033A
          ELISA SynblockBUF034A100 mlE
          BUF034A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          ELISA BSA BlockBUF032A100 mlE
          BUF032A
          ELISA UltrablockBUF033A100 mlE
          BUF033A
          ELISA SynblockBUF034A100 mlE
          BUF034A

          Recommended Positive Controls

            Histology Controls

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