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Protocol: PK Bridging ELISA For Use With Anti-Rituximab Antibodies
Pharmacokinetic (PK) Bridging ELISA: For use with anti-rituximab monoclonal antibody products HCA186 and MCA2260P
This method provides a procedure for carrying out a PK ELISA with anti-rituximab antibodies, product codes HCA186 (capture antibody) and MCA2260P (detection antibody), and using rituximab monoclonal antibody for the standard curve.
The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.