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Protocol: PK Bridging ELISA For Use With Anti-Trastuzumab Antibodies HCA166, HCA168
Pharmacokinetic (PK) Bridging ELISA: For use with anti-trastuzumab monoclonal antibody products HCA166 and HCA168
This method provides a procedure for carrying out a PK ELISA with anti-trastuzumab antibodies, product codes HCA168 (capture antibody) and HCA166 (detection antibody), and using trastuzumab monoclonal antibody for the standard curve.
The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
Lynx Rapid Conjugation Kit® is a registered trademark of Bio-Rad AbD Serotec Ltd.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.