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PK Bridging ELISA For Use With Anti-Trastuzumab Antibodies HCA169, HCA270
Pharmacokinetic (PK) Bridging ELISA: For use with anti-trastuzumab monoclonal antibody products HCA169 and HCA270
This method provides a procedure for carrying out a PK ELISA with anti-trastuzumab antibodies, product codes HCA169 (capture antibody), and HCA270 (detection antibody, IgG4 isotype) using trastuzumab for the standard curve and an HRP conjugated anti-human IgG4 antibody, MCA2098P for detection.
The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
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