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Protocol: PK Bridging ELISA For Use With Anti-Cetuximab Antibodies
Pharmacokinetic (PK) Bridging ELISA: For use with anti-cetuximab monoclonal antibody products HCA220 and HCA221P
This method provides a procedure for carrying out a PK ELISA with anti-cetuximab antibodies, product codes HCA220 (capture antibody) and HCA221P (detection antibody), and using cetuximab monoclonal antibody for the standard curve.
The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.