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Protocol: PK Bridging ELISA for Use with Anti-Eculizumab Antibodies
Pharmacokinetic (PK) Bridging ELISA: for Use with Anti-Eculizumab Monoclonal Antibodies HCA312 and HCA313P
This method provides a procedure for carrying out a PK ELISA with Anti-Eculizumab Antibodies, catalog number HCA312 (capture antibody) and HCA313P (detection antibody), and using eculizumab for the standard curve. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.