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Protocol: PK Bridging ELISA For Use With Anti-Bevacizumab Antibodies
Pharmacokinetic (PK) Bridging ELISA: For use with anti-bevacizumab monoclonal antibodies HCA182 and HCA184P
This method provides a procedure for carrying out a PK ELISA with anti-bevacizumab antibodies, product codes HCA182 (capture antibody) and HCA184P (detection antibody), and using bevacizumab monoclonal antibody for the standard curve.
The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
MaxiSorp™ and QuantaBlu™ are trademarks of Thermo Fisher Scientific.
Tween® is a registered trademark of ICI Americas Inc.