Abatacept antibody | AbD37058ia

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Human anti Abatacept

Human Anti-Abatacept Antibody is a recombinant, inhibitory antibody for use in bioanalytical assays for abatacept and biosimilars. It can be used as a detection antibody in a PK bridging ELISA, and as a positive control in an ADA bridging ELISA.

Product Type
Monoclonal Antibody
Clone
AbD37058ia
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
HCA335
Datasheet
E
SDS
0.1 mg loader

Human Anti-Abatacept Antibody, clone AbD37058ia is a recombinant antibody that specifically recognizes the fusion protein drug abatacept as well as cytotoxic T-lymphocyte antigen-4 (CTLA-4), also known as CD152. The antibody binds to free abatacept and can be used to measure free abatacept or abatacept biosimilars in patient samples.

Clone AbD37058ia is a fully human recombinant monoclonal antibody with an IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. Additionally, the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. It is recommended as the detection antibody, paired with Mouse Anti-Human CD152 Antibody, clone BNI3 (MCA1724) as the capture reagent.

Abatacept (Orencia) is a recombinant fusion protein consisting of the extra-cellular domain of human CTLA-4, fused to the modified Fc (hinge, CH2, and CH3 domains) portion of human IgG1. It is used to treat several autoimmune diseases, including rheumatoid arthritis, psoriatic arthritis and juvenile idiopathic arthritis, by inhibiting optimal T-cell activation. It binds to CD80 and CD86 on antigen-presenting cells and blocks the costimulatory interaction with CD28 on T cells.

View a summary of all anti-abatacept antibodies

Product Details

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Abatacept
Affinity
The monovalent intrinsic affinity of AbD37058ia was measured as KD = 1 nM by real time, label free molecular interaction analysis on immobilized abatacept.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Orencia is a trademark of Bristol-Myers Squibb Company.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Abatacept antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD37058ia can be used in an indirect ELISA or as detection antibody for abatacept in a bridging ELISA together with MCA1724 as the capture reagent. It can also be used as a calibrator in an anti-drug antibody assay.

Protocol: PK bridging ELISA

Protocol: ADA bridging ELISA

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Abatacept HCA336 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody

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