Abatacept antibody | AbD37058ia
Human anti Abatacept
Human Anti-Abatacept Antibody is a recombinant, inhibitory antibody for use in bioanalytical assays for abatacept and biosimilars. It can be used as a detection antibody in a PK bridging ELISA, and as a positive control in an ADA bridging ELISA.
- Product Type
- Monoclonal Antibody
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Clone AbD37058ia is a fully human recombinant monoclonal antibody with an IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. Additionally, the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. It is recommended as the detection antibody, paired with Mouse Anti-Human CD152 Antibody, clone BNI3 (MCA1724) as the capture reagent.
Abatacept (Orencia) is a recombinant fusion protein consisting of the extra-cellular domain of human CTLA-4, fused to the modified Fc (hinge, CH2, and CH3 domains) portion of human IgG1. It is used to treat several autoimmune diseases, including rheumatoid arthritis, psoriatic arthritis and juvenile idiopathic arthritis, by inhibiting optimal T-cell activation. It binds to CD80 and CD86 on antigen-presenting cells and blocks the costimulatory interaction with CD28 on T cells.
View a summary of all anti-abatacept antibodies
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- The monovalent intrinsic affinity of AbD37058ia was measured as KD = 1 nM by real time, label free molecular interaction analysis on immobilized abatacept.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
Orencia is a trademark of Bristol-Myers Squibb Company.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- For research purposes only
Applications of Abatacept antibody
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- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- Clone AbD37058ia can be used in an indirect ELISA or as detection antibody for abatacept in a bridging ELISA together with MCA1724 as the capture reagent. It can also be used as a calibrator in an anti-drug antibody assay.
Protocol: PK bridging ELISA
Protocol: ADA bridging ELISA
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Hispec Assay Diluent||BUF049A||E IY||50 ml|
|Human anti Abatacept||HCA336||E||0.1 mg|
|LYNX Rapid HRP Antibody Conjugation Kit||LNK002P||CJ||3 Conjugations For 400µg Antibody|
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