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Protocol: PK Bridging ELISA for Use with Anti-Denosumab Antibodies
Pharmacokinetic (PK) Bridging ELISA: for use with anti-denosumab monoclonal antibody products HCA280 or HCA282 and HCA283P
This method provides a procedure for carrying out a PK ELISA with Anti-Denosumab Antibodies, HCA280 or HCA282 (capture antibodies) and HCA283P (detection antibody), and using denosumab for the standard curve. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.