Protocol: ADA Bridging ELISA for Use with Anti-Pembrolizumab Antibodies
Anti-Drug Antibody (ADA) Bridging ELISA: for Use with Anti-Pembrolizumab Monoclonal Antibodies HCA297 and HCA298
This method provides a procedure for generating an ADA ELISA standard curve with an Anti-Pembrolizumab Antibody, catalog number HCA297 or HCA298 using pembrolizumab antibody for capture and detection. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.