Pembrolizumab antibody | AbD30685_hIgG1

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Human anti Pembrolizumab:HRP

Human Anti-Pembrolizumab Antibody is a recombinant, inhibitory, anti-idiotypic antibody in IgG1 format for use in drug level monitoring. It is labeled with HRP, and is recommended as the detection antibody in a PK bridging ELISA with HCA296 as capture antibody.

Human anti Pembrolizumab

Human Anti-Pembrolizumab Antibody is a recombinant, inhibitory, anti-idiotypic antibody in IgG1 format for use in drug level monitoring and immunogenicity testing. It can be used as detection antibody in a PK bridging ELISA and as a calibrator in an anti-drug antibody assay.

Product Type
Monoclonal Antibody
Clone
AbD30685_hIgG1
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA297P E 0.1 mg
HCA297 E 0.1 mg
Human Anti-Pembrolizumab Antibody, clone AbD30685_hIgG1 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free humanized monoclonal antibody pembrolizumab. The antibody does not recognize recombinant human programmed cell death 1 (PD-1) or pembrolizumab in complex with recombinant human PD-1 and can be used to measure free pembrolizumab levels in serum from patients.

Clone AbD30685_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. A pair of anti-pembrolizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD30689 (HCA296) in monovalent Fab format as the capture antibody.

Pembrolizumab (Keytruda) is a humanized (IgG4/kappa) antibody with the heavy chain mutation S228P (IgG4-Pro). Pembrolizumab is used to treat inoperable or metastatic melanoma, metastatic non-small cell lung cancer (NSCLC), and as a second-line treatment for head and neck squamous cell carcinoma (HNSCC). For NSCLC, pembrolizumab is a first line treatment if the cancer overexpresses PDL1 and has no mutations in EGFR or ALK. Pembrolizumab is a checkpoint inhibitor and acts as an immunomodulator by blocking ligand activation of PD-1 receptor on T cells, thereby activating T cells to attack the cancer. It blocks PD-1 interaction with PD-1 ligand (PD-L1) and PD-1 ligand 2 (PD-L2).

View a summary of all anti-pembrolizumab antibodies

Product Details

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is conjugated to horseradish peroxidase (HRP) and supplied as liquid.
Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Pembrolizumab
Affinity
The monovalent intrinsic affinity of AbD30685_hIgG1 was measured as KD = 5 nM by real time, label free molecular interaction analysis on immobilized pembrolizumab.
Approx. Protein Concentrations
Antibody concentration 0.1 mg/ml
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at -70oC.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Keytruda is a trademark of Merck & Co.
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Keytruda is a trademark of Merck & Co.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Pembrolizumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD30685_hIgG1 can be used in a direct ELISA or as detection antibody for pembrolizumab in a bridging ELISA together with HCA296 as the capture reagent.

Protocol: PK bridging ELISA to measure free drug
ELISA
Clone AbD30685_hIgG1 can be used in a direct or indirect ELISA or as detection antibody for pembrolizumab in a bridging ELISA together with HCA296 as the capture reagent. Furthermore, it may be used as a calibrator for the set-up of an anti-drug antibody assay.

Protocol: PK bridging ELISA to measure free drug and anti-drug antibody assay

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Recombinant Human IgG1 Lambda HCA049 0.1 mg E
Recombinant Human IgG1 Lambda HCA049 0.1 mg E

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Pembrolizumab HCA296 0.1 mg E
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P 0.2 mg C* E
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Pembrolizumab HCA296 0.1 mg E
LYNX Rapid HRP Antibody Conjugation Kit LNK002P 3 Conjugations For 400µg Antibody CJ
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P 0.2 mg C* E
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