Pembrolizumab antibody | AbD30689_hIgG1




Human anti Pembrolizumab
Human Anti-Pembrolizumab Antibody is a recombinant, inhibitory, anti-idiotypic antibody in IgG1 format for use in immunogenicity testing. It can be used as a calibrator in an anti-drug antibody assay.
- Product Type
- Monoclonal Antibody
- Clone
- AbD30689_hIgG1
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
HCA298 | E | 0.1 mg |
![]() |
Clone AbD30689_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay.
Pembrolizumab (Keytruda) is a humanized (IgG4/kappa) antibody with the heavy chain mutation S228P (IgG4-Pro). Pembrolizumab is used to treat inoperable or metastatic melanoma, metastatic non-small cell lung cancer (NSCLC), and as a second-line treatment for head and neck squamous cell carcinoma (HNSCC). For NSCLC, pembrolizumab is a first line treatment if the cancer overexpresses PDL1 and has no mutations in EGFR or ALK. Pembrolizumab is a checkpoint inhibitor and acts as an immunomodulator by blocking ligand activation of PD-1 receptor on T cells, thereby activating T cells to attack the cancer. It blocks PD-1 interaction with PD-1 ligand (PD-L1) and PD-1 ligand 2 (PD-L2).
View a summary of all anti-pembrolizumab antibodies
Product Details
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Pembrolizumab
- Affinity
- The monovalent intrinsic affinity of AbD30689_hIgG1 was measured as KD = 0.6 nM by real time, label free molecular interaction analysis on immobilized pembrolizumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany. Keytruda is a trademark of Merck & Co.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Pembrolizumab antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD30689_hIgG1 can be used in a direct or indirect ELISA or as a detection antibody for pembrolizumab. It may also be used as a surrogate positive control to generate a standard curve for an anti-drug antibody assay.
Protocol: anti-drug antibody assay
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Recombinant Human IgG1 Lambda Allotype G1m3 | HCA049 | E | 0.1 mg |
![]() |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
Human anti Pembrolizumab | HCA296 | E | 0.1 mg |
![]() |
|
Human anti Pembrolizumab | HCA297 | E | 0.1 mg |
![]() |
|
LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | ||
Mouse anti Human IgG (Fc) CH2 Domain:HRP | MCA647P | C* E | 0.2 mg |
![]() |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra