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Pembrolizumab antibody | AbD30685_hIgG1

Human anti Pembrolizumab:HRP

Product Type
Monoclonal Antibody
Clone
AbD30685_hIgG1
Isotype
IgG1
Specificity
Pembrolizumab

Product Code Applications Pack Size List Price Your Price Qty
HCA297P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader

Human Anti-Pembrolizumab Antibody, clone AbD30685_hIgG1 is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free humanized monoclonal antibody pembrolizumab. The antibody does not recognize recombinant human programmed cell death 1 (PD-1) or pembrolizumab in complex with recombinant human PD-1 and can be used to measure free pembrolizumab levels in serum from patients.

Clone AbD30685_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. A pair of anti-pembrolizumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody paired with clone AbD30689 (HCA296) in monovalent Fab format as the capture antibody.

Pembrolizumab (Keytruda) is a humanized (IgG4/kappa) antibody with the heavy chain mutation S228P (IgG4-Pro). Pembrolizumab is used to treat inoperable or metastatic melanoma, metastatic non-small cell lung cancer (NSCLC), and as a second-line treatment for head and neck squamous cell carcinoma (HNSCC). For NSCLC, pembrolizumab is a first line treatment if the cancer overexpresses PDL1 and has no mutations in EGFR or ALK. Pembrolizumab is a checkpoint inhibitor and acts as an immunomodulator by blocking ligand activation of PD-1 receptor on T cells, thereby activating T cells to attack the cancer. It blocks PD-1 interaction with PD-1 ligand (PD-L1) and PD-1 ligand 2 (PD-L2).

View a summary of all anti-pembrolizumab antibodies

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This antibody is conjugated to horseradish peroxidase (HRP) and supplied as liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Pembrolizumab
Affinity
The intrinsic affinity of the monovalent form of Human anti Pembrolizumab antibody, clone AbD30685_hIgG1 is KD = 5 nM as measured by real time, label free molecular interaction analysis on immobilized Pembrolizumab.
Approx. Protein Concentrations
Antibody concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Keytruda is a trademark of Merck & Co.

This product is shipped frozen.
When ready to use, thaw and aliquot the sample as needed. Store aliquots at -70°C, if available, otherwise store at -20°C. It is not recommended to keep aliquots at 4°C for more than one week.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Human anti Pembrolizumab antibody, clone AbD30685_hIgG1 can be used in a direct ELISA or as detection antibody for pembrolizumab in a bridging ELISA together with HCA296 as the capture reagent.

Protocol: PK bridging ELISA to measure free drug.

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human IgG1 Lambda Allotype G1m3 HCA049 E 0.1 mg loader
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Description Recombinant Human IgG1 Lambda Allotype G1m3

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Pembrolizumab HCA296 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Pembrolizumab
Mouse anti Human IgG (Fc) CH2 Domain:HRP MCA647P C * E 0.2 mg loader
List Price Your Price
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Description Mouse anti Human IgG (Fc) CH2 Domain:HRP

References for Pembrolizumab antibody

  1. Engström, J. (2023) Gyrolab Immunoassays: Miniaturization, Automation, and Integration into a Rapid Workflow.
    Methods Mol Biol. 2612: 109-27.

Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

HCA297P

152056 163273 165375 165380 INN1803

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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