MCM2 Proliferation Detection Antibody

Versatile antibody for multiple applications

There are three key markers for the identification of cell proliferation; Ki67, MCM2 and PCNA. The cell cycle mini-review provides information on all three of these markers, including their role and expression during the cell cycle.

BrdU incorporation is also a popular method to detect cell proliferation; incorporated BrdU is detected with anti-BrdU antibodies, such as the Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483) or Rabbit Anti-BrdU Antibody (AHP2405). To learn more about the anti-BrdU antibody range, staining protocols and references visit our BrdU Antibodies for Cell Proliferation Detection page.

MCM2 is one of six members of the MCM protein family, which play a key role in the initiation of DNA replication. MCM2 is highly expressed in early G1, moderately expressed in S, G2, and M phases, and absent during G0 (Maiorano et al. 2006). In addition, MCM2 demonstrates distinct cellular localization patterns that can be measured in cycling cells. The localization and expression pattern of MCM2 therefore makes it an ideal proliferation marker.

Mouse anti Human MCM2 antibody, clone CRCT2.1 (MCA1859) has been used successfully for the detection of proliferating human cells. This antibody can be used in IHC-P and WB applications.

To determine whether your cell of interest is proliferating, pair MCM2 antibody with another cell specific primary antibody. Below are example cell specific antibodies compatible with MCM2. A list of secondary antibodies recommended for use with these pairs are listed in table 2.

For single staining, follow the standard protocol for that application; for double staining use the Rabbit Anti-MCM2 Proliferation Pairs Staining Procedure.
 

Table 1. Proliferation pairs.

The following secondary antibodies are recommended for use with these primary antibodies.

Table 2. Secondary Antibodies.

References

• Maiorano D et al. (2006). MCM proteins and DNA replication. Curr Opin Cell Biol 18, 130-136.