MCM2 Proliferation Detection Antibody

Versatile antibody for multiple applications

There are three key markers for the identification of cell proliferation; Ki67, MCM2 and PCNA. The cell cycle mini-review provides information on all three of these markers, including their role and expression during the cell cycle.

BrdU incorporation is also a popular method to detect cell proliferation; incorporated BrdU is detected with anti-BrdU antibodies, such as the Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483) or Rabbit Anti-BrdU Antibody (AHP2405). Click here to learn more about the anti-BrdU antibody range, staining protocols and references.

MCM2 is one of six members of the MCM protein family, which play a key role in the initiation of DNA replication. MCM2 is highly expressed in early G1, moderately expressed in S, G2, and M phases, and absent during G0 (Maiorano et al. 2006). In addition, MCM2 demonstrates distinct cellular localization patterns that can be measured in cycling cells. The localization and expression pattern of MCM2 therefore makes it an ideal proliferation marker.

Rabbit Anti-Mini-Chromosome Maintenance Protein 2 (MCM2) Polyclonal Antibody (AHP2404) has been generated to identify proliferating human, mouse or rat cells. This versatile antibody can be used across multiple applications including IHC-F, IHC-P, IF and WB. More information on MCM2 antibody, including the datasheet can be found here.

To determine whether your cell of interest is proliferating, pair MCM2 antibody with another cell specific primary antibody. Below are example cell specific antibodies compatible with MCM2. A list of  secondary antibodies recommended for use with these pairs are listed in table 2.

For single staining, follow the standard protocol for that application; for double staining use the Rabbit Anti-MCM2 Proliferation Pairs Staining Procedure.


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Table 1. Proliferation pairs.

Target Cells

Marker

Target Species

Host

Isotype

Application

Catalog #

Macrophage

CD68

Human

Mouse

IgG1

IHC-P

MCA5709

B cell

CD19

Human

Mouse

IgG1

IHC-P, IHC-F

MCA1940

CD4 T cell

CD4

Human

Mouse

IgG1

IHC-P, IHC-F

MCA1267

CD8 T cell

CD8

Human

Mouse

IgG2b

IHC-P, IHC-F, WB

MCA1817

Pan T cell

CD3

Human

Rat

IgG1

IHC-P, IHC-F, WB

MCA1477

Hematopoietic stem cell

CD34

Human

Mouse

IgG1

IHC-P, IHC-F, WB

MCA547

Cancer associated fibroblast

CD140b

Human

Mouse

IgG1

IHC-P, IHC-F, WB

7460-3104

NK cell

CD56

Human

Mouse

IgG1

IHC-F

MCA591

Activated endothelial cells and platelets

CD62P

Human

Mouse

IgG1

IHC-P, IHC-F, WB

MCA796

Breast tumor

ERB1

Human

Mouse

IgG2a

IHC-P, IHC-F

MCA1974

Breast tumor

Progesterone receptor

Human

Mouse

IgG1

IHC-P, WB

MCA1800

Breast tumor

HER2

Human

Rat

IgG2a

IHC-P, IHC-F, WB

MCA1788

Neuronal precursors

Nestin

Human

Mouse

IgG1

IHC-P, IHC-F, WB

OBT1610

Neuronal precursors

Nestin

Rat, Mouse

Mouse

IgG1

IHC-P, IHC-F, WB

6625-1010

Simple epithelium

Cytokeratin 18

Human

Mouse

IgG1

IHC-P, IHC-F

MCA1864

Simple epithelium

Cytokeratin 19

Human

Mouse

IgG1

IHC-P, IHC-F, WB

MCA2145

The following secondary antibodies are recommended for use with these primary antibodies.

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Table 2. Secondary Antibodies.

Primary Antibody

Chromogenic Detection

Fluorescent Detection

MCM2 Rabbit IgG

644004 (Alk Phos), STAR54 (HRP)

STAR36D488, STAR36D549

Cell Specific Antibody Mouse IgG

STAR117A (Alk Phos), STAR117P (HRP)

STAR117D488, STAR117D549

Cell Specific antibody Rat IgG

STAR131A (Alk Phos), STAR72 (HRP)

STAR71D549

Find out about cell proliferation and how to analyze it here.

View our range of MCM2 antibodies, available in different formats and suitable for multiple applications including western blotting, immunofluorescence and immunohistochemistry.

MCM2 Image gallery


ImmunofluorescenceImmunofluorescence

Immunofluorescence staining of formalin fixed paraffin embedded human colon adenocarcinoma, using Rabbit Anti-MCM2 Antibody (AHP2404) at 2 μg/ml. As the secondary antibody, Sheep Anti-Rabbit IgG DyLight 549 Conjugated Antibody (STAR36D549) was used. Co-staining was performed with Mouse Anti-Human Cytokeratin 18 (MCA1864H). Goat Anti-Mouse IgG (H/L) DyLight 488 Conjugated Antibody (STAR117D488) was used as the secondary. PureBlu DAPI (1351303) was used as a nuclear counterstain (blue). 

Immunohistochemical - paraffin embedded tissueImmunohistochemical - paraffin embedded tissue

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil using Rabbit Anti-MCM2 Antibody (AHP2404) at 2 μg/ml. Detection was performed using the Histar Detection Kit (STAR3000A).

Immunohistochemical - frozen tissueImmunohistochemical - frozen tissue

Immunohistochemical analysis of frozen human tonsil using Rabbit Anti-MCM2 Antibody (AHP2404) at 2 μg/ml. Detection was performed using the Histar Detection Kit (STAR3000A).

Western blotWestern blot

Western blot analysis of HeLa cell lysate (Lane 2), NIH/3T3 cell lysate (Lane 3) and PC12 cell lysate (lane 4), run under reducing conditions and probed with Rabbit Anti-MCM2 Antibody (AHP2404) at 1 μg/ml. As the secondary antibody HRP conjugated, Goat Anti-Rabbit IgG Antibody (STAR208P) was used at 1/10 000. Rabbit Anti-MCM2 detects a band of approximately 125 kDa.

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What Is Cell Proliferation and How to Analyze It? View MCM2 Antibody Range

References

  • Maiorano D et al. (2006). MCM proteins and DNA replication. Curr Opin Cell Biol 18, 130-136.