HER2/neu antibody | ICR55

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Rat anti Human HER2/neu

Rat anti Human HER2/neu:Alexa Fluor®700

Product Type
Monoclonal Antibody
Clone
ICR55
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1788T C F IP P WB 25 µg
MCA1788 C F IP P WB 0.2 mg
MCA1788A700 F 100 Tests/1ml
Rat anti Human HER2/neu antibody, clone ICR55 recognizes epitope E of human HER2/neu, otherwise known as CD340 and c-ErbB-2, a proto-oncogene expressed at the cell surface of a range of tumor cells, often associated with poor prognosis, and an increased risk of metastasis.

HER2/neu is used in conjunction with antibodies recognizing both Estrogen and Progesterone receptors, for the classification of breast cancer tumors, and to determine patient prognosis and course of treatment.

Excessive HER2/neu is present in around 20% to 25% of invasive breast cancers, referred to as HER2-positive cancer, and can help determine whether drugs such as trastuzumab (Herceptin) or lapatinib (Tykerb), might be beneficial.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 700 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 25 µg
0.02%Sodium Azide
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P04626 Related reagents
Entrez Gene
ERBB2 Related reagents
GO Terms
GO:0003677 DNA binding
GO:0016021 integral to membrane
GO:0004716 receptor signaling protein tyrosine kinase activity
GO:0005006 epidermal growth factor receptor activity
GO:0043125 ErbB-3 class receptor binding
GO:0005524 ATP binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0007507 heart development
GO:0008022 protein C-terminus binding
GO:0008283 cell proliferation
GO:0042802 identical protein binding
GO:0014065 phosphatidylinositol 3-kinase cascade
GO:0019903 protein phosphatase binding
GO:0048015 phosphatidylinositol-mediated signaling
GO:0042060 wound healing
GO:0043235 receptor complex
GO:0043406 positive regulation of MAP kinase activity
GO:0045449 regulation of transcription
GO:0045765 regulation of angiogenesis
GO:0045785 positive regulation of cell adhesion
GO:0046777 protein autophosphorylation
GO:0046982 protein heterodimerization activity
GO:0048471 perinuclear region of cytoplasm
GO:0050679 positive regulation of epithelial cell proliferation
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of HER2/neu antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Product Specific References

References for HER2/neu antibody

  1. Styles, J.M. et al. (1990) Rat monoclonal antibodies to the external domain of the product of the C-erbB-2 proto-oncogene.
    Int J Cancer. 45 (2): 320-4.
  2. Dean, C.J. et al. (1993) Rat MAbs to the product of the c-erbB-2 proto-oncogene for diagnosis and therapy in breast cancer.
    Cell Biophys. 22 (1-3): 111-27.
  3. Sain, N. et al. (2006) Potentiation of paclitaxel activity by the HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin in human ovarian carcinoma cell lines with high levels of activated AKT.
    Mol Cancer Ther. 5 (5): 1197-208.