CD19 antibody | LT19
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors.
(A) Immunofluorescent staining of human melanoma and normal human skin tissue sections; anti-CD19 (red; B cell marker) and anti-p-STAT3 (green). Scale bars, 20 μm.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors.
(B) B cells in primary tumor sites impact overall tumor STAT3 activity. Confocal microscopic images showing primary melanoma tumor tissue staining of B cells and p-STAT3 (left), with quantification of CD19 and p-STAT3 positive cells (right). Scale bars, 20 μm. Total ten microscopic fields (10 X) were examined for each tumor section; n = 2.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 express pro-angiogenic genes and accumulate around microvessels in human tumors.
(A) B cells are important for expression of pro-angiogenic genes within human prostate tumor tissues. The density of B cells around tumor vasculature in prostate tumor tissue was determined by immunofluorescent staining using anti-CD19 and anti-CD31 antibodies (top); scale bars, 20 μm. Real-time RT-PCR measuring RNA expression levels of pro-angiogenic genes in the consecutive human prostate tumor tissue sections (bottom). The relative amount of mRNA is normalized to 18S and compared to RNA levels in tumor tissues with high p-STAT3, which is designated as 1; means ± SD, n = 2.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 express pro-angiogenic genes and accumulate around microvessels in human tumors.
(B) Immunofluorescent staining of human prostate tumor tissue sections showing B cells and microvessels with CD31+ endothelial cells (green). Scale bars, 100μm in the original (top) and 20μm in the enlarged (bottom).
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors and their density in the tumor tissues reflects overall STAT3 activity.
(A) Immunofluorescent staining of many other types of human cancers; anti-CD19 (red; B cell marker) and anti-p-STAT3 (green). Scale bars, 50μm in the original and 10μm in the enlarged.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunofluorescence.
Image caption:
B cells with activated STAT3 accumulate in human tumors and their density in the tumor tissues reflects overall STAT3 activity.
(B) Immunofluorescent staining showing CD19- and p-STAT3 (green) and nuclei (DAPI, blue). Scale bars, 10μm.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunohistochemistry on formalin fixed, paraffin embedded tissue sections..
Image caption:
B cells accumulate around microvessels in human prostate tumors.
IHC images showing the accumulation of CD19-positive B cells in human prostate tumor tissues; H&E staining of the consecutive tissue sections.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD19 antibody, clone LT19 (MCA1940) used to label human B cells by immunohistochemistry on formalin fixed, paraffin embedded tissue sections..
Image caption:
B cells with activated STAT3 express pro-angiogenic genes and accumulate around microvessels in human tumors.
(C) B cells around the microvessels display persistently activated STAT3. IHC showing B cells and p-STAT3-positive cells in the same area of human prostate tumor tissues; scale bars, 200 μm. H&E staining of the consecutive tissue sections. Microvessel-like structures are marked by red dots; scale bars, 200μm in the original and 50μm in the enlarged.
From: Yang C, Lee H, Pal S, Jove V, Deng J, Zhang W, et al. (2013)
B Cells Promote Tumor Progression via STAT3 Regulated-Angiogenesis.
PLoS ONE 8(5): e64159.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F IF P ResetMouse anti Human CD19
- Product Type
- Monoclonal Antibody
- Clone
- LT19
- Isotype
- IgG1
- Specificity
- CD19
Antibody Quality and Performance Guarantee
| Mouse anti Human CD19 antibody, clone LT19 recognizes human CD19 also known as T-cell surface antigen Leu-12 or B-lymphocyte surface antigen B4. CD19 is a ~95 kDa type I single pass transmembrane glycoprotein expressed on follicular dendritic cells and B-cells during maturation but is lost on development into plasma cells (de Rie et al. 1989). CD19 is the broadest lineage specific marker for B cells and functions as a B-cell co-receptor in conjunction with CD21 (Bradbury et al. 1992), CD9, CD81 and CD82 (Horváth et al. 1998). CD19 is implicated in the down-regulation of B cell growth and proliferation (Pezzutto et al. 1987). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD19 antibody
-
Hughes, G.J. et al. (2007) Virus immunocapture provides evidence of CD8 lymphocyte-derived HIV-1 in vivo.
AIDS. 21: 1507-13. -
Allen, J.S. et al. (2009) Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture.
Diabetes. 58: 138-45. -
McIntosh, K. et al. (2006) The immunogenicity of human adipose-derived cells: temporal changes in vitro.
Stem Cells. 24: 1246-53. -
Sengstake, S. et al. (2006) CD21 and CD62L shedding are both inducible via P2X7Rs.
Int Immunol. 18 (7): 1171-8. -
Villarroel Dorrego, M. et al. (2006) Transfection of CD40 in a human oral squamous cell carcinoma keratinocyte line upregulates immune potency and costimulatory molecules.
Br J Dermatol. 154: 231-8. -
Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
Blood. 118: 348-57. -
Lacal, P.M. et al. (2013) Glucocorticoid-induced tumor necrosis factor receptor family-related ligand triggering upregulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 and promotes leukocyte adhesion.
J Pharmacol Exp Ther. 347: 164-72. -
Franz, B. et al. (2011) Ex vivo characterization and isolation of rare memory B cells with antigen tetramers.
Blood. 118: 348-57.
View The Latest Product References
-
Girbl, T. et al. (2013) CD40-mediated activation of chronic lymphocytic leukemia cells promotes their CD44-dependent adhesion to hyaluronan and restricts CCL21-induced motility.
Cancer Res. 73: 561-70. -
Hertzberg, L. et al. (2010) Down syndrome acute lymphoblastic leukemia, a highly heterogeneous disease in which aberrant expression of CRLF2 is associated with mutated JAK2: a report from the International BFM Study Group.
Blood. 115: 1006-17. -
Kakko, T. et al. (2011) Inflammatory effects of blood leukocytes: association with vascular function in neuropeptide Y proline 7-genotyped type 2 diabetes patients.
Diab Vasc Dis Res. 8: 221-8. -
Dorvignit, D. et al. (2012) Expression and biological characterization of an anti-CD20 biosimilar candidate antibody: a case study.
MAbs. 4 (4): 488-96. -
Karlsen, M. et al. (2015) TLR-7 and -9 Stimulation of Peripheral Blood B Cells Indicate Altered TLR Signalling in Primary Sjögren's Syndrome Patients by Increased Secretion of Cytokines.
Scand J Immunol. 82 (6): 523-31. -
Clark, L.E. et al. (2018) Vaccine-elicited receptor-binding site antibodies neutralize two New World hemorrhagic fever arenaviruses.
Nat Commun. 9 (1): 1884. -
Gu, Y. et al. (2019) Defining the structural basis for human alloantibody binding to human leukocyte antigen allele HLA-A*11:01.
Nat Commun. 10 (1): 893. -
Yang, C. et al. (2013) B cells promote tumor progression via STAT3 regulated-angiogenesis.
PLoS One. 8 (5): e64159.
- RRID
- AB_323226
- UniProt
- P15391
- Entrez Gene
- CD19
- GO Terms
- GO:0005515 protein binding
- GO:0005057 receptor signaling protein activity
- GO:0005887 integral to plasma membrane
- GO:0006968 cellular defense response
MCA1940GA
151083
MCA1940
151082
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