CD62P antibody | AK-6
FITC conjugated Mouse anti Human CD62P antibody, clone AK-6 (MCA796F) used to assess activationof CD41 positive platelets by flow cytometry.
Image caption:
Dot plot: Log SSC (Side Scatter) versus Log FL2 (Filter 2) (in orange). A gate was drawn around CD41+ events, delineating the platelet population. FL1 Histogram: count versus log FL1 (in green). The gate CD41+ was applied to analyze FL1. ‘%R’ represents the percentage of CD-62p positive events. (A) Upper panels, TRAP-activated platelets (TRAP Ctl+); lower panels, resting platelets (PBS Ctl−). A total of 10,000 platelets (CD41+ events) were analyzed in each run and the percentage of activated platelets (CD62p positive events) was evaluated (FL1 histogram). For each series, a cursor indicating the activation threshold was placed at the intersection of the FL1 histograms of the negative control (PBS Ctl−) and the positive control (TRAP Ctl+) and the cut-off was determined. In this example, with resting platelets (PBS Ctl−), we observed 11.7% CD62p-positive events corresponding to spontaneous platelet activation and electronic noise. After platelet activation with TRAP (TRAP Ctl+), we observed 86.5% CD62p-positive events corresponding to maximal platelet activation. (B) Left panels: Platelets activated with a highly HIT positive plasma. We observed 88.1% CD62p-positive events with 0.3 IU/mL. UFH (upper panel) and 12.9% CD62p-positive events with 100 IU/mL UFH (lower panel). The HEPLA index was 100% Middle panels: Platelets activated with intermediately HIT positive plasma. We observed 53.4% CD62p-positive events with 0.3 IU/mL UFH (upper panel) and 18.4% CD62p-positive events with 100 IU/mL UFH (lower panel). The HEPLA index was 47%. Right panels: Platelets incubated with HIT negative plasma. We observed 13.5% CD62p-positive events with 0.3 IU/mL UFH (upper panel) and 12.5% with 100 IU/mL UFH (lower panel). The HEPLA index was 1%. TRAP: Thrombin Receptor Agonist Peptide, PBS: Phosphate-buffered saline, HIT: Heparin-induced thrombocytopenia, UFH: Unfractioned heparin, HEPLA: Heparin Platelet Activation.
From: Tardy-Poncet, B.; Montmartin, A.; Piot, M.; Alhenc-Gelas, M.; Nguyen, P.; Elalamy, I.; Greinacher, A.; Maistre, E.D.; Lasne, D.; Horellou, M.-H.; Le Gal, G.; Lecompte, T.; Tardy, B.; on behalf of the GFHT-HIT Study Group.
Functional Flow Cytometric Assay for Reliable and Convenient Heparin-Induced Thrombocytopenia Diagnosis in Daily Practice.
Biomedicines 2021, 9, 332.
doi: 10.3390/biomedicines9040332
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
F P ResetMouse anti Human CD62P
- Product Type
- Monoclonal Antibody
- Clone
- AK-6
- Isotype
- IgG1
- Specificity
- CD62P
Antibody Quality and Performance Guarantee
| Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P, also known as P-selectin, Granule membrane protein 140, GMP140, Leukocyte-endothelial cell adhesion molecule 3 or Platelet activation dependent granule-external membrane protein. CD62P is a 830 amino acid, including a 41 amino acid signal peptide, ~140 kDa single pass type I transmembrane glycoprotein expressed on activated platelets and endothelial cell CD62P plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the platelet alpha granule and is rapidly translocated to the plasma membrane upon activation (Stenberg et al. 1985). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA796: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA796GA: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human platelet membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
1/1000 | 1/5000 |
| Immunohistology - Paraffin 1 | |
1/500 | 1/2000 |
| Western Blotting | |
- 1Staining may be enhanced using Sodium Citrate buffer pH6.0
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Immunohistology
- This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections but staining may be enhanced using Sodium Citrate buffer pH6.0
- Histology Positive Control Tissue
- Tonsil
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD62P antibody
-
Skinner, M.P. et al. (1989) Characterization of human platelet GMP-140 as a heparin-binding protein.
Biochem Biophys Res Commun. 164 (3): 1373-9. -
Skinner, M.P. et al. (1991) GMP-140 binding to neutrophils is inhibited by sulfated glycans.
J Biol Chem. 266 (9): 5371-4. -
Dunlop, L.C. et al. (1992) Characterization of GMP-140 (P-selectin) as a circulating plasma protein.
J Exp Med. 175 (4): 1147-50. -
Theoret, J.F. et al. (2001) P-selectin antagonism with recombinant p-selectin glycoprotein ligand-1 (rPSGL-Ig) inhibits circulating activated platelet binding to neutrophils induced by damaged arterial surfaces.
J Pharmacol Exp Ther. 298: 658-64 -
Turner, C.P. et al. (2003) The role of P-selectin in the immune destruction of platelets.
Br J Haematol. 121: 623-31. -
Roos-Engstrand, E. et al. (2005) Increased expression of p38 MAPK in human bronchial epithelium after lipopolysaccharide exposure.
Eur Respir J. 25 (5): 797-803. -
Norling, L.V. et al. (2008) Inhibitory control of endothelial galectin-1 on in vitro and in vivo lymphocyte trafficking.
FASEB J. 22: 682-90. -
Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
Blood. 112 (6): 2512-9.
View The Latest Product References
-
Wassmer, S.C. at al. (2008) Platelet-induced clumping of Plasmodium falciparum-infected erythrocytes from Malawian patients with cerebral malaria-possible modulation in vivo by thrombocytopenia.
J Infect Dis. 197: 72-8. -
Kornerup, K.N. et al. (2010) Circulating platelet-neutrophil complexes are important for subsequent neutrophil activation and migration.
J Appl Physiol. 109: 758-67. -
van Nispen tot Pannerden, H. et al. (2010) The platelet interior revisited: electron tomography reveals tubular alpha-granule subtypes.
Blood. 116: 1147-56. -
Knipe, L. et al. (2010) A revised model for the secretion of tPA and cytokines from cultured endothelial cells.
Blood. 116 (12): 2183-91. -
Kitaya, K. & Yasuo, T. (2010) Aberrant expression of selectin E, CXCL1, and CXCL13 in chronic endometritis.
Mod Pathol. 23 (8): 1136-46. -
Christersson, C. et al. (2013) Evaluation of microparticles in whole blood by multicolour flow cytometry assay.
Scand J Clin Lab Invest. 73(3): 229-39. -
Xiong, G.M. et al. (2015) Imparting electroactivity to polycaprolactone fibers with heparin-doped polypyrrole: Modulation of hemocompatibility and inflammatory responses.
Acta Biomater. 23: 240-9. -
Liao, Y. et al. (2017) Tailoring of TiO2 films by H2SO4 treatment and UV irradiation to improve anticoagulant ability and endothelial cell compatibility.
Colloids Surf B Biointerfaces. 155: 314-22. -
Cipok, M. et al. (2019) Pathogenic heparin-induced thrombocytopenia and thrombosis (HIT) antibodies determined by rapid functional flow cytometry.
Eur J Haematol. 103 (3): 225-233. -
Jiang, T. et al. (2019) Hyaluronic Acid Nanoparticle Composite Films Confer Favorable Time-Dependent Biofunctions for Vascular Wound Healing.
ACS Biomater Sci Eng. 5 (4): 1833-48. -
Tardy-Poncet, B. et al. (2021) Functional Flow Cytometric Assay for Reliable and Convenient Heparin-Induced Thrombocytopenia Diagnosis in Daily Practice
Biomedicines. 9 (4): 332.
Further Reading
-
Bevilacqua, M.P. & Nelson, R.M. (1993) Selectins.
J Clin Invest. 91 (2): 379-87.
- Synonyms
- P-SELECTIN
- RRID
- AB_1125273
- UniProt
- P16109
- Entrez Gene
- SELP
- GO Terms
- GO:0001530 lipopolysaccharide binding
- GO:0002576 platelet degranulation
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0005624 membrane fraction
- GO:0005625 soluble fraction
- GO:0008201 heparin binding
- GO:0050829 defense response to Gram-negative bacterium
- GO:0009897 external side of plasma membrane
- View More GO Terms
- GO:0010572 positive regulation of platelet activation
- GO:0030168 platelet activation
- GO:0031088 platelet dense granule membrane
- GO:0031092 platelet alpha granule membrane
- GO:0032496 response to lipopolysaccharide
- GO:0033691 sialic acid binding
- GO:0042806 fucose binding
- GO:0043208 glycosphingolipid binding
- GO:0070492 oligosaccharide binding
MCA796
148930
152767
MCA796GA
152472
1709
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