Estrogen Receptor Beta 1 antibody | PPG5/10

Mouse anti Human Estrogen Receptor Beta 1

Product Type
Monoclonal Antibody
Clone
PPG5/10
Isotype
IgG2a
Specificity
Estrogen Receptor Beta 1

Product Code Applications Pack Size List Price Your Price Qty
MCA1974GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C IF P * 0.1 mg loader
List Price Your Price
loader
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Mouse anti Human estrogen receptor beta 1, clone PPG5/10 recognizes the estrogen receptor beta 1 (ERB1), one of several isoforms of estrogen receptor (ER) which can be used in conjunction with progesterone receptor (PR) and HER2/neu (CD340), to help determine both prognosis and the effectiveness of hormonal therapy, in breast cancer patients. Prognosis and survival rate varies greatly depending on cancer type, stage and treatment.

Mouse anti Human estrogen receptor beta 1, clone PPG5/10 is increasingly being utilized in the immunohistochemical staining of formal fixed paraffin embedded tissue, in preference to ligand binding assays, for the detection of ER/PR. ER/PR is present in approximately 75% to 80% of breast tumors, and breast cancer cells with these receptors depend on the hormones estrogen and/or progesterone to grow (Saunders et al., 2002 and Nelson et al., 2017).

Mouse anti Human estrogen receptor beta 1, clone PPG5/10 can be used to demonstrate the nuclear presence of ERB1 within cells of the breast (Shaaban et al., 2008), ovary, uterus, oviduct, testis, prostate, bladder and male reproductive ducts.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
New World primate
Llama
Rat
Marmoset
Pig
Sheep
Cynomolgus monkey
Rhesus Monkey
Turtle
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Synthetic peptide sequence CSPAEDSKSKEGSQNPQSQ from the C-terminal region of the human estrogen receptor beta 1 isoform.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections;
    0.05M glycine/EDTA pH 8.0 see (See Carpino et al.) or sodium citrate buffer pH 6.0 are recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Ovary or breast carcinoma

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References for Estrogen Receptor Beta 1 antibody

  1. Miller, W.R. et al. (2006) Oestrogen receptor beta and neoadjuvant therapy with tamoxifen: prediction of response and effects of treatment.
    Br J Cancer. 94: 1333-8.
  2. Nakopoulou, L. et al. (2004) The favourable prognostic value of oestrogen receptor beta immunohistochemical expression in breast cancer.
    J Clin Pathol. 57: 523-8.
  3. Rago, V. et al. (2007) Cytochrome P450arom, androgen and estrogen receptors in pig sperm.
    Reprod Biol Endocrinol. 5: 23.
  4. Rago, V. et al. (2009) Identification of ERbeta1 and ERbeta2 in human seminoma, in embryonal carcinoma and in their adjacent intratubular germ cell neoplasia.
    Reprod Biol Endocrinol. 7: 56.
  5. Savolainen, S. et al. (2007) Delay of postnatal maturation sensitizes the mouse prostate to testosterone-induced pronounced hyperplasia: protective role of estrogen receptor-beta.
    Am J Pathol. 171: 1013-22.
  6. Moore, J.T. et al. (1998) Cloning and characterization of human estrogen receptor beta isoforms.
    Biochem Biophys Res Commun. 247 (1): 75-8.
  7. Torlakovic, E. et al. (2002) Prostate carcinoma expression of estrogen receptor-beta as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score.
    Hum Pathol. 33 (6): 646-51.
  8. Saunders, P.T. et al. (2002) Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.
    Br J Cancer. 86 (2): 250-6.
  9. View The Latest Product References
  10. Schiessl, B. et al. (2005) Expression of endothelial NO synthase, inducible NO synthase, and estrogen receptors alpha and beta in placental tissue of normal, preeclamptic, and intrauterine growth-restricted pregnancies.
    J Histochem Cytochem. 53 (12): 1441-9.
  11. Wong NA et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.
    J Pathol. 207 (1): 53-60.
  12. Fixemer, T. et al. (2003) Differential expression of the estrogen receptor beta (ERbeta) in human prostate tissue, premalignant changes, and in primary, metastatic, and recurrent prostatic adenocarcinoma.
    Prostate. 54: 79-87.
  13. Wood, C.E. et al. (2006) Hyperplastic and neoplastic lesions of the mammary gland in macaques.
    Vet Pathol. 43 (4): 471-83.
  14. Juengel, J. L. et al. (2006) Oestrogen receptor alpha and beta, androgen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep.
    Reproduction. 131: 81-92.
  15. Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.
    BMC Cancer. 9: 330.
  16. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.
    Eur J Endocrinol. 157 (2): 239-44.
  17. Bianchi, C. et al. (2010) Endometrial population of oestrogen receptors alpha and beta and progesterone receptors A and B during the different phases of the follicular wave of llamas (Lama glama).
    Reprod Domest Anim. 45 (5): 872-80.
  18. Marotti, J.D. et al. (2010) Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: results from the Nurses' Health Study.
    Mod Pathol. 23: 197-204.
  19. Saunders, P.T. et al. (2000) Differential expression of estrogen receptor-alpha and -beta and androgen receptor in the ovaries of marmosets and humans.
    Biol Reprod. 63 (4): 1098-105.
  20. Asgari, M. and Morakabati, A. (2011) Estrogen receptor beta expression in prostate adenocarcinoma.
    Diagn Pathol. 6: 61.
  21. Fowler, P.A. et al. (2011) Development of steroid signaling pathways during primordial follicle formation in the human fetal ovary.
    J Clin Endocrinol Metab. 96: 1754-62.
  22. Mafuvadze, B. et al. (2011) Apigenin Prevents Development of Medroxyprogesterone Acetate-Accelerated 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumors in Sprague-Dawley Rats.
    Cancer Prev Res (Phila). 4: 1316-24.
  23. Silvestri, A. and Fraser, H.M. (2007) Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy.
    Reproduction. 134: 341-53.
  24. Bombail, V. et al. (2008) Estrogen receptor related beta is expressed in human endometrium throughout the normal menstrual cycle.
    Hum Reprod. 23: 2782-90.
  25. Nagai H et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
    PLoS One. 10 (7): e0131923.
  26. Leska A et al. (2015) Estradiol concentration and the expression of estrogen receptors in the testes of the domestic goose (Anser anser f. domestica) during the annual reproductive cycle.
    Domest Anim Endocrinol. 51: 96-104.
  27. Faustino-Rocha, A.I. et al. (2016) Effects of lifelong exercise training on mammary tumorigenesis induced by MNU in female Sprague-Dawley rats.
    Clin Exp Med. Apr19 [Epub ahead of print]
  28. Grindstad, T. et al. (2016) Estrogen receptors α and β and aromatase as independent predictors for prostate cancer outcome.
    Sci Rep. 6: 33114.
  29. Skjefstad K et al. (2016) Prognostic relevance of estrogen receptor α, β and aromatase expression in non-small cell lung cancer.
    Steroids. pii: S0039-128X(16)30040-X. [Epub ahead of print]
  30. Ciucci, A. et al. (2018) Estrogen receptor β: Potential target for therapy in adult granulosa cell tumors?
    Gynecol Oncol. 150 (1): 158-165.
  31. Hawse, J.R. et al. (2020) Optimized immunohistochemical detection of estrogen receptor beta using two validated monoclonal antibodies confirms its expression in normal and malignant breast tissues.
    Breast Cancer Res Treat. 179 (1): 241-9.
  32. Hu, M. et al. (2020) Alterations of endometrial epithelial-mesenchymal transition and MAPK signalling components in women with PCOS are partially modulated by metformin in vitro..
    Mol Hum Reprod. 26 (5): 312-326.
  33. Austin, D. et al. (2018) Estrogen receptor-beta is a potential target for triple negative breast cancer treatment.
    Oncotarget. 9 (74): 33912-30.
  34. Dong, W.W. et al. (2018) Reduced expression of oestrogen receptor-β is associated with tumour invasion and metastasis in oestrogen receptor-α-negative human papillary thyroid carcinoma.
    Int J Exp Pathol. 99 (1): 15-21.
  35. Rago, V. et al. (2018) Expression of oestrogen receptors (GPER, ESR1, ESR2) in human ductuli efferentes and proximal epididymis.
    Andrology. 6 (1): 192-8.
  36. Younes, M. et al. (2018) Expression of estrogen receptor beta isoforms in pancreatic adenocarcinoma.
    Oncotarget. 9 (102): 37715-20.
  37. Hu, M. et al. (2019) Uterine glycolytic enzyme expression is affected by knockout of different estrogen receptor subtypes.
    Biomed Rep. 11 (4): 135-44.
  38. Ellerbrock, R.E. et al. (2021) Steroidogenic Enzyme and Steroid Receptor Expression in the Equine Accessory Sex Glands.
    Animals (Basel). 11 (8): 2322.
  39. Bedir, Ö. et al. (2021) Plane of nutrition and FSH-induced superovulation affect the expression of steroid hormone receptors and growth factors in caruncular tissue of non-pregnant sheep.
    Domestic Animal Endocrinology. : Sep 10 [Epub ahead of print]
  40. Pérez-Ruiz, E. et al. (2018) Expression and Prognostic Value of Oestrogen Receptor Beta in Colorectal Cancer.
    Pathol Oncol Res. 24 (4): 871-879.
  41. Le Coronet, C. et al. (2020) Circulating 27-hydroxycholesterol and breast cancer tissue expression of CYP27A1, CYP7B1, LXR-β, and ERβ: results from the EPIC-Heidelberg cohort.
    Breast Cancer Res. 22 (1): 23.
  42. Hexiao, T. et al. (2021) Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway.
    Aging (Albany NY). 13 (2): 2604-2625.

Further Reading

  1. Nelson, A.W. et al. (2017) Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity.
    Mol Cell Endocrinol. 440: 138-150.
  2. Andersson, S. et al. (2017) Insufficient antibody validation challenges oestrogen receptor beta research.
    Nat Commun. 8: 15840.

Immunofluorescence

Immunohistology - Paraffin

UniProt
Q92731
Entrez Gene
ESR2
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