Estrogen Receptor Beta 1 antibody | PPG5/10

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Mouse anti Human Estrogen Receptor Beta 1

Mouse anti Human Estrogen Receptor Beta 1

Mouse anti Human Estrogen Receptor Beta 1

Product Type
Monoclonal Antibody
Clone
PPG5/10
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1974ST C IF P* 0.1 ml
MCA1974GA C IF P* 0.1 mg
MCA1974 C IF P* 2 ml
Mouse anti Human estrogen receptor beta 1, clone PPG5/10 recognizes the estrogen receptor beta 1 (ERB1), one of several isoforms of estrogen receptor (ER) which can be used in conjunction with progesterone receptor (PR) and HER2/neu (CD340), to help determine both prognosis and the effectiveness of hormonal therapy, in breast cancer patients. Prognosis and survival rate varies greatly depending on cancer type, stage and treatment.

Mouse anti Human estrogen receptor beta 1, clone PPG5/10 is increasingly being utilized in the immunohistochemical staining of formal fixed paraffin embedded tissue, in preference to ligand binding assays, for the detection of ER/PR. ER/PR is present in approximately 75% to 80% of breast tumors, and breast cancer cells with these receptors depend on the hormones estrogen and/or progesterone to grow (Saunders, P. et al., 2002 and Nelson, A.W. et al., 2017).

Mouse anti Human estrogen receptor beta 1, clone PPG5/10 can be used to demonstrate the nuclear presence of ERB1 within cells of the breast (Shaaban, A.M.et al., 2008), ovary, uterus, oviduct, testis, prostate, bladder and male reproductive ducts.

BE ADVISED: Publications (Nelson, A.W. et al., 2017 and Andersson, S. et al., 2017) have queried the validity of clone PPG5/10. Please refer to the references section of this product's web page or datasheet for further information.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Primate
New World primate
Llama
Rat
Marmoset
Pig
Sheep
Cynomolgus monkey
Rhesus Monkey
Turtle
Goose
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Concentrated Tissue Culture Supernatant - liquid
Product Form
Purified IgG - liquid
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence CSPAEDSKSKEGSQNPQSQ from the C-terminal region of the human estrogen receptor beta 1 isoform.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q92731 Related reagents
Entrez Gene
ESR2 Related reagents
Regulatory
For research purposes only

Applications of Estrogen Receptor Beta 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/5 1/50
Western Blotting
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1
Western Blotting
Flow Cytometry
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1 Neat 1/20
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    0.05M glycine/EDTA pH8 see (See Carpino et al.) or sodium citrate buffer pH 6.0 are recommended for this purpose.
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections;
    0.05M glycine/EDTA pH 8.0 see (See Carpino et al.) or sodium citrate buffer pH 6.0 are recommended for this purpose.
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
    0.05M glycine/EDTA pH8 see (See Carpino et al.) or sodium citrate buffer pH 6.0 are recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Ovary
Histology Positive Control Tissue
Ovary or breast carcinoma
Histology Positive Control Tissue
Ovary

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for Estrogen Receptor Beta 1 antibody

  1. Moore, J.T. et al. (1998) Cloning and characterization of human estrogen receptor beta isoforms.
    Biochem Biophys Res Commun. 247 (1): 75-8.
  2. Torlakovic, E. et al. (2002) Prostate carcinoma expression of estrogen receptor-beta as detected by PPG5/10 antibody has positive association with primary Gleason grade and Gleason score.
    Hum Pathol. 33 (6): 646-51.
  3. Saunders, P.T. et al. (2002) Expression of oestrogen receptor beta (ERbeta1) protein in human breast cancer biopsies.
    Br J Cancer. 86 (2): 250-6.
  4. Schiessl, B. et al. (2005) Expression of endothelial NO synthase, inducible NO synthase, and estrogen receptors alpha and beta in placental tissue of normal, preeclamptic, and intrauterine growth-restricted pregnancies.
    J Histochem Cytochem. 53 (12): 1441-9.
  5. Wong NA et al. (2005) ERbeta isoform expression in colorectal carcinoma: an in vivo and in vitro study of clinicopathological and molecular correlates.
    J Pathol. 207 (1): 53-60.
  6. Fixemer, T. et al. (2003) Differential expression of the estrogen receptor beta (ERbeta) in human prostate tissue, premalignant changes, and in primary, metastatic, and recurrent prostatic adenocarcinoma.
    Prostate. 54: 79-87.
  7. Wood, C.E. et al. (2006) Hyperplastic and neoplastic lesions of the mammary gland in macaques.
    Vet Pathol. 43 (4): 471-83.
  8. Juengel, J. L. et al. (2006) Oestrogen receptor alpha and beta, androgen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep.
    Reproduction. 131: 81-92.
  9. Collins, F. et al. (2009) Expression of oestrogen receptors, ERalpha, ERbeta, and ERbeta variants, in endometrial cancers and evidence that prostaglandin F may play a role in regulating expression of ERalpha.
    BMC Cancer. 9: 330.
  10. Carpino, A. et al. (2007) Detection of aromatase and estrogen receptors (ERalpha, ERbeta1, ERbeta2) in human Leydig cell tumor.
    Eur J Endocrinol. 157 (2): 239-44.
  11. Bianchi, C. et al. (2010) Endometrial population of oestrogen receptors alpha and beta and progesterone receptors A and B during the different phases of the follicular wave of llamas (Lama glama).
    Reprod Domest Anim. 45 (5): 872-80.
  12. Marotti, J.D. et al. (2010) Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: results from the Nurses' Health Study.
    Mod Pathol. 23: 197-204.
  13. Saunders, P.T. et al. (2000) Differential expression of estrogen receptor-alpha and -beta and androgen receptor in the ovaries of marmosets and humans.
    Biol Reprod. 63 (4): 1098-105.
  14. Asgari, M. and Morakabati, A. (2011) Estrogen receptor beta expression in prostate adenocarcinoma.
    Diagn Pathol. 6: 61.
  15. Fowler, P.A. et al. (2011) Development of steroid signaling pathways during primordial follicle formation in the human fetal ovary.
    J Clin Endocrinol Metab. 96: 1754-62.
  16. Mafuvadze, B. et al. (2011) Apigenin Prevents Development of Medroxyprogesterone Acetate-Accelerated 7,12-Dimethylbenz(a)anthracene-Induced Mammary Tumors in Sprague-Dawley Rats.
    Cancer Prev Res (Phila). 4: 1316-24.
  17. Silvestri, A. and Fraser, H.M. (2007) Oestrogen and progesterone receptors in the marmoset endometrium: changes during the ovulatory cycle, early pregnancy and after inhibition of vascular endothelial growth factor, GnRH or ovariectomy.
    Reproduction. 134: 341-53.
  18. Bombail, V. et al. (2008) Estrogen receptor related beta is expressed in human endometrium throughout the normal menstrual cycle.
    Hum Reprod. 23: 2782-90.
  19. Nagai H et al. (2015) Pulmonary Macrophages Attenuate Hypoxic Pulmonary Vasoconstriction via β3AR/iNOS Pathway in Rats Exposed to Chronic Intermittent Hypoxia.
    PLoS One. 10 (7): e0131923.
  20. Leska A et al. (2015) Estradiol concentration and the expression of estrogen receptors in the testes of the domestic goose (Anser anser f. domestica) during the annual reproductive cycle.
    Domest Anim Endocrinol. 51: 96-104.
  21. Faustino-Rocha, A.I. et al. (2016) Effects of lifelong exercise training on mammary tumorigenesis induced by MNU in female Sprague-Dawley rats.
    Clin Exp Med. Apr19 [Epub ahead of print]
  22. Grindstad, T. et al. (2016) Estrogen receptors α and β and aromatase as independent predictors for prostate cancer outcome.
    Sci Rep. 6: 33114.
  23. Skjefstad K et al. (2016) Prognostic relevance of estrogen receptor α, β and aromatase expression in non-small cell lung cancer.
    Steroids. pii: S0039-128X(16)30040-X. [Epub ahead of print]

Further Reading

  1. Nelson, A.W. et al. (2017) Comprehensive assessment of estrogen receptor beta antibodies in cancer cell line models and tissue reveals critical limitations in reagent specificity.
    Mol Cell Endocrinol. 440: 138-150.
  2. Andersson, S. et al. (2017) Insufficient antibody validation challenges oestrogen receptor beta research.
    Nat Commun. 8: 15840.