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IgG antibody

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Anti Rat IgG (Mouse Adsorbed) Antibody thumbnail image 1 Anti Rat IgG (Mouse Adsorbed) Antibody thumbnail image 2 Anti Rat IgG (Mouse Adsorbed) Antibody thumbnail image 3
Anti Rat IgG (Mouse Adsorbed) Antibody gallery image 1
Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b (MCA2293) at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha (MCA78G) is included as a loading control. Detection is with Goat anti Rat IgG:Dylight® (STAR71D800GA).
Anti Rat IgG (Mouse Adsorbed) Antibody gallery image 2
Figure A. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Rat IgG2a isotype control (MCA6005) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). Figure B. FITC conjugated Mouse anti Canine CD3 (MCA1774F) and purified Mouse anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Anti Rat IgG (Mouse Adsorbed) Antibody gallery image 3
Figure A. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a DyLight 649 (STAR71D649) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Rat anti Canine CD90 (MCA1036GA) detected with Goat anti Rat IgG2a PE (STAR71D649) and Mouse anti Canine CD3 FITC (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

Goat anti Rat IgG (Mouse Adsorbed)

Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR71
Datasheet
E F
MSDS
0.5 mg loader

STAR71D800GA
Datasheet
F IF WB
MSDS
0.1 mg loader

Goat anti Rat IgG (Mouse Adsorbed) antibody recognizes rat IgG. Cross-reactivity with mouse IgG has been minimised by adsorption.

Product Details

Target Species
Rat
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to DyLight®800 - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Rat IgG.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P20759
P20762
P20761
P20760
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 5ug/ml
Flow Cytometry
Immunofluorescence
Western Blotting 1/10000 1/50000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit anti Goat IgG (Fc):FITC STAR122F F 1 mg loader
Rabbit anti Goat IgG (Fc):HRP STAR122P C E WB 1 mg loader

Product Specific References

References for IgG antibody

  1. Yang, X. et al. (2010) The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells.
    J Neuroinflammation. 7: 54.
  2. Tamayo, J. et al. (2001) Chemical sensors and biosensors in liquid environment based on microcantilevers with amplified quality factor.
    Ultramicroscopy. 86: 167-73.
  3. Pérez-Bosque A et al. (2004) Dietary plasma protein affects the immune response of weaned rats challenged with S. aureus Superantigen B.
    J Nutr. 134: 2667-72.
  4. Balan, P. et al. (2010) Immunomodulatory effects of ovine serum immunoglobulin in the growing rat.
    Animal. 4: 1702-8.
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