Nestin antibody | 10C2

Mouse anti Human Nestin

Product Type
Monoclonal Antibody
Clone
10C2
Isotype
IgG1
Specificity
Nestin

Product Code Applications Pack Size List Price Your Price Qty
OBT1610
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C P WB 0.1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human Nestin antibody, clone 10C2 recognizes nestin, a large intermediate filament protein originally identified by (Hockfield and McKay 1985). Nestin is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Antibody purified from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1/50 1/200
Western Blotting 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Suggested positive control: WI-38 or U251 cell lysate.

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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)

References for Nestin antibody

  1. Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.
    J Neurosci. 5 (12): 3310-28.
  2. Martínez-Navarrete, G.C. et al. (2008) Gradual morphogenesis of retinal neurons in the peripheral retinal margin of adult monkeys and humans
    J Comp Neurol. 511: 557-80.
  3. González-Garza, M.T. et al. (2013) Differentiation of CD133+ stem cells from amyotrophic lateral sclerosis patients into preneuron cells.
    Stem Cells Transl Med. 2 (2): 129-35.
  4. Aljammal, K. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades
    J Cytol Histol. 6:313.
  5. Sterlacci, W. et al. (2014) Putative stem cell markers in non-small-cell lung cancer: a clinicopathologic characterization.
    J Thorac Oncol. 9 (1): 41-9.
  6. Nowak, A. et al. (2017) Nestin-positive microvessel density is an independent prognostic factor in breast cancer.
    Int J Oncol. Jun 26. [Epub ahead of print]
  7. Arranz, L. et al. (2014) Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms.
    Nature. 512 (7512): 78-81.
  8. Del Toro, R. et al. (2016) Nestin(+) cells direct inflammatory cell migration in atherosclerosis.
    Nat Commun. 7: 12706.
  9. View The Latest Product References
  10. Forte, D. et al. (2020) Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy.
    Cell Metab. 32 (5): 829-843.e9.
  11. Tampakis, A. et al. (2021) Nestin and CD34 expression in colorectal cancer predicts improved overall survival.
    Acta Oncol. 60 (6): 727-34.

Immunohistology - Paraffin

RRID
AB_2151139
UniProt
P48681
Entrez Gene
NES
GO Terms
GO:0005882 intermediate filament
GO:0005198 structural molecule activity
GO:0005737 cytoplasm
GO:0007420 brain development
GO:0019215 intermediate filament binding
GO:0030844 positive regulation of intermediate filament depolymerization
GO:0031076 embryonic camera-type eye development
GO:0043066 negative regulation of apoptosis
GO:2000179 positive regulation of neural precursor cell proliferation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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