Nestin antibody | 10C2










Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P48681
- Entrez Gene
- NES
- GO Terms
- GO:0005882 intermediate filament
- GO:0005198 structural molecule activity
- GO:0005737 cytoplasm
- GO:0007420 brain development
- GO:0019215 intermediate filament binding
- GO:0030844 positive regulation of intermediate filament depolymerization
- GO:0031076 embryonic camera-type eye development
- GO:0043066 negative regulation of apoptosis
- GO:2000179 positive regulation of neural precursor cell proliferation
- Regulatory
- For research purposes only
Applications of Nestin antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen | |||
Immunohistology - Paraffin | 1/50 | 1/200 | |
Western Blotting | 1/500 | 1/1000 |
- Western Blotting
- Suggested positive control: WI-38 (ATCC# CCL75) or U251 cell lysate.
Secondary Antibodies Available
Product Specific References
References for Nestin antibody
-
Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.
J Neurosci. 5 (12): 3310-28. -
Martínez-Navarrete, G.C. et al. (2008) Gradual morphogenesis of retinal neurons in the peripheral retinal margin of adult monkeys and humans
J Comp Neurol. 511: 557-80. -
González-Garza, M.T. et al. (2013) Differentiation of CD133+ stem cells from amyotrophic lateral sclerosis patients into preneuron cells.
Stem Cells Transl Med. 2 (2): 129-35. -
Aljammal, K. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades
J Cytol Histol. 6:313. -
Sterlacci, W. et al. (2014) Putative stem cell markers in non-small-cell lung cancer: a clinicopathologic characterization.
J Thorac Oncol. 9 (1): 41-9. -
Nowak, A. et al. (2017) Nestin-positive microvessel density is an independent prognostic factor in breast cancer.
Int J Oncol. Jun 26. [Epub ahead of print] -
Arranz, L. et al. (2014) Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms.
Nature. 512 (7512): 78-81. -
Del Toro, R. et al. (2016) Nestin(+) cells direct inflammatory cell migration in atherosclerosis.
Nat Commun. 7: 12706. -
Forte, D. et al. (2020) Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy.
Cell Metab. 32 (5): 829-843.e9.
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