Nestin antibody | 10C2
Mouse anti Human Nestin antibody, clone 10C2 (OBT1610) used for the evaluation of nestin expression in aortic tissues by immunohistochemistry on formalin fixed paraffin embedded tissue sections.
Image caption:
Nestin+ cells in the aortic adventitia and media express Mcp1 in mice and humans
Immunohistochemistry of (g–i) NESTIN and (h–j) MCP1 in consecutive cross sections from human (g,h) coronary and (i,j) carotid artery samples. Red arrowheads depict cells that express both proteins in consecutive sections. Scale bar 100 μm.
From: Del Toro R, Chèvre R, Rodríguez C, Ordóñez A, Martínez-González J, Andrés V, Méndez-Ferrer S.
Nestin(+) cells direct inflammatory cell migration in atherosclerosis.
Nat Commun. 2016 Sep 2;7:12706.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Overview of representative punches of control and tumor tissue samples stained with Hematoxylin/Eosin (H/E) or immunostained for the 24 specific proteins grouped by categories.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015)
Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of fetal, epileptic and gliotic tissues immunostained for nestin, SPARC and IDH1(R132H). IDH1(R132H) was not expressed in these control brain samples, whereas nestin was highly expressed in fetal tissue, not in epilepsy (except one single cell shown in inlet), but was present in reactive glial cells in gliotic tissue (black arrows) and in microvessels (white arrows). SPARC was expressed in reactive glial cells in gliotic tissue (black arrows), as well as in rare cells in epileptic tissue (black arrows), in addition to some vascular cells (white arrows). Original magnification x400. Scale bar: 100 μm.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015)
Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of fetal, epileptic and gliotic tissues immunostained for nestin, SPARC and IDH1(R132H). IDH1(R132H) was not expressed in these control brain samples, whereas nestin was highly expressed in fetal tissue, not in epilepsy (except one single cell shown in inlet), but was present in reactive glial cells in gliotic tissue (black arrows) and in microvessels (white arrows). SPARC was expressed in reactive glial cells in gliotic tissue (black arrows), as well as in rare cells in epileptic tissue (black arrows), in addition to some vascular cells (white arrows). Original magnification x400. Scale bar: 100 μm.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015)
Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of two cases of low grade astrocytomas immunostained for nestin, SPARC and IDH1(R132H). Case 1 expressed the mutated IDH1 protein, whereas case 2 was clearly IDH1(R132H)-negative. Both cases were nestin and SPARC immunopositive. The center (c) and periphery (p) of the corresponding tumors are depicted for each case. Original magnification x400. Scale bar: 100 μm.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of two cases of anaplastic astrocytoma immunostained for nestin, SPARC and IDH1(R132H). Case 1 was positive for all three markers, whereas case 2 was IDH1(R132H) negative and low for SPARC expression. For both cases, the center (c) and periphery (p) of the tumors are depicted. Original magnification x400. Scale bar: 100 μm.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015)
Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of tumors in pGBM and sGBM immunostained for nestin, SPARC and IDH1(R132H). The case of sGBM was positive for all three markers, whereas pGBM was IDH1(R132H) negative. The center (c) and periphery (p) of the tumors are shown. Original magnification x400. Scale bar: 100 μm.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015)
Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of the tumor centers of two cases of sGBM with low SPARC immunostaining. The two cases (case 1 and 2) showed high cellularity, with strong nuclear expression of PCNA and p53 in almost all cells, in addition to the expression of IDH1(R132H). Case 1 showed low number of nestin-positive cells, whereas case 2 revealed strong nestin immunostaining in all cells at the “hot spots” indicated by black arrowheads. Original magnification x400. Scale bar: 100 μm.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015)
Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10 C2 (OBT1610) used for the identification of nestin expressing cells in human biopsies by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative micrographs of medulloblastoma and metastasis immunostained for nestin, SPARC and IDH1(R132H). Nestin was mainly observed expressed by tumor vasculature and some rare tumor cells. SPARC expression was strong in medulloblastoma cells and the cells constituting the rosette in the metastasis. No IDH1(R132H) staining was observed in these two cases. Original magnification x400. Scale bar: 100 μm.
From: Aljammal K, Ritz MF, Ramadoss A, Sauter G, Boulay JL, et al. (2015)
Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades.
J Cytol Histol 6: 313.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10C2 (OBT1610) used to identify Nestin+ niches in human bone marrow biopsy samples by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Unlike Bulk BM Stromal Cells, Nestin+ Niche Cells Are Preserved in Human and Murine AML and Promote Leukemogenesis
(A–D) Representative examples of immunohistochemistry for human NESTIN (brown) and human CD34 (pink) in BM samples from AML patients. CD34− AML (A), CD34+ AML ([B] and [C]), and MLL-AF9+ AML (D) show increased NESTIN+ niches (arrows) and CD34+ vessels (arrowheads). Scale bar, 10 μm (A–C), 100 μm (D).
(E) Quantification of NESTIN+ niches from (A and B) (control n = 12; MLL-AF9− AML n = 56; MLL-AF9+ AML n = 5). ∗p <0.05, ∗∗p <0.01, one-way ANOVA and Bonferroni comparisons.
From: Forte D, et al.
Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy.
Cell Metab. 2020 Nov 3;32(5):829-843.e9.
doi: 10.1016/j.cmet.2020.09.001.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human Nestin antibody, clone 10C2 recognizes nestin, a large intermediate filament protein originally identified by (Hockfield and McKay 1985). Nestin is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P48681
- Entrez Gene
- NES
- GO Terms
- GO:0005882 intermediate filament
- GO:0005198 structural molecule activity
- GO:0005737 cytoplasm
- GO:0007420 brain development
- GO:0019215 intermediate filament binding
- GO:0030844 positive regulation of intermediate filament depolymerization
- GO:0031076 embryonic camera-type eye development
- GO:0043066 negative regulation of apoptosis
- GO:2000179 positive regulation of neural precursor cell proliferation
- Regulatory
- For research purposes only
Applications of Nestin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | 1/50 | 1/200 | |
| Western Blotting | 1/500 | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Suggested positive control: WI-38 or U251 cell lysate.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Nestin antibody
-
Hockfield, S. & McKay, R.D. (1985) Identification of major cell classes in the developing mammalian nervous system.
J Neurosci. 5 (12): 3310-28. -
Martínez-Navarrete, G.C. et al. (2008) Gradual morphogenesis of retinal neurons in the peripheral retinal margin of adult monkeys and humans
J Comp Neurol. 511: 557-80. -
González-Garza, M.T. et al. (2013) Differentiation of CD133+ stem cells from amyotrophic lateral sclerosis patients into preneuron cells.
Stem Cells Transl Med. 2 (2): 129-35. -
Aljammal, K. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades
J Cytol Histol. 6:313. -
Sterlacci, W. et al. (2014) Putative stem cell markers in non-small-cell lung cancer: a clinicopathologic characterization.
J Thorac Oncol. 9 (1): 41-9. -
Nowak, A. et al. (2017) Nestin-positive microvessel density is an independent prognostic factor in breast cancer.
Int J Oncol. Jun 26. [Epub ahead of print] -
Arranz, L. et al. (2014) Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms.
Nature. 512 (7512): 78-81. -
Del Toro, R. et al. (2016) Nestin(+) cells direct inflammatory cell migration in atherosclerosis.
Nat Commun. 7: 12706. -
Forte, D. et al. (2020) Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy.
Cell Metab. 32 (5): 829-843.e9.
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