Nestin antibody | 10C2
Mouse anti Human Nestin
- Product Type
- Monoclonal Antibody
- Clone
- 10C2
- Isotype
- IgG1
- Specificity
- Nestin
Mouse anti Human Nestin antibody, clone 10C2 recognizes nestin, a large intermediate filament protein originally identified by (Hockfield and McKay 1985). Nestin is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Antibody purified from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Frozen | |||
Immunohistology - Paraffin | 1/50 | 1/200 | |
Western Blotting | 1/500 | 1/1000 |
- Western Blotting
- Suggested positive control: WI-38 or U251 cell lysate.
References for Nestin antibody
-
Martínez-Navarrete, G.C. et al. (2008) Gradual morphogenesis of retinal neurons in the peripheral retinal margin of adult monkeys and humans
J Comp Neurol. 511: 557-80. -
González-Garza, M.T. et al. (2013) Differentiation of CD133+ stem cells from amyotrophic lateral sclerosis patients into preneuron cells.
Stem Cells Transl Med. 2 (2): 129-35. -
Aljammal, K. (2015) Combined Expression of Nestin and SPARC Identifies In Situ Tumor Cells in Astrocytic Tumors of all Grades
J Cytol Histol. 6:313. -
Sterlacci, W. et al. (2014) Putative stem cell markers in non-small-cell lung cancer: a clinicopathologic characterization.
J Thorac Oncol. 9 (1): 41-9. -
Nowak, A. et al. (2017) Nestin-positive microvessel density is an independent prognostic factor in breast cancer.
Int J Oncol. 51 (2): 668-76. -
Arranz, L. et al. (2014) Neuropathy of haematopoietic stem cell niche is essential for myeloproliferative neoplasms.
Nature. 512 (7512): 78-81. -
Del Toro, R. et al. (2016) Nestin(+) cells direct inflammatory cell migration in atherosclerosis.
Nat Commun. 7: 12706. -
Forte, D. et al. (2020) Bone Marrow Mesenchymal Stem Cells Support Acute Myeloid Leukemia Bioenergetics and Enhance Antioxidant Defense and Escape from Chemotherapy.
Cell Metab. 32 (5): 829-843.e9.
View The Latest Product References
-
Tampakis, A. et al. (2021) Nestin and CD34 expression in colorectal cancer predicts improved overall survival.
Acta Oncol. 60 (6): 727-34.
- RRID
- AB_2151139
- UniProt
- P48681
- Entrez Gene
- NES
- GO Terms
- GO:0005882 intermediate filament
- GO:0005198 structural molecule activity
- GO:0005737 cytoplasm
- GO:0007420 brain development
- GO:0019215 intermediate filament binding
- GO:0030844 positive regulation of intermediate filament depolymerization
- GO:0031076 embryonic camera-type eye development
- GO:0043066 negative regulation of apoptosis
- GO:2000179 positive regulation of neural precursor cell proliferation
- View More GO Terms
OBT1610
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