CD140b antibody | PR7212

100% Secure


Mouse anti Human CD140b

Product Type
Monoclonal Antibody
Clone
PR7212
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7460-3104 C F IF IP P WB 0.1 mg
Mouse anti Human CD140b antibody, clone PR7212 recognizes the extracellular domain of the human PDGF receptor beta subunit. This was confirmed in binding studies using several different cell lines and by its ability to immunoprecipitate PDGF receptor beta complexed with 125I-PDGF-BB (Hart et al. 1987). Mouse anti Human CD140b antibody, clone PR7212 does not recognize PDGF receptor alpha.

PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Baboon
Rat
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Human skin fibroblast cell membrane extracts.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P09619 Related reagents
Entrez Gene
PDGFRB Related reagents
GO Terms
GO:0004992 platelet activating factor receptor activity
GO:0005019 platelet-derived growth factor beta-receptor activity
GO:0005021 vascular endothelial growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005161 platelet-derived growth factor receptor binding
GO:0005524 ATP binding
GO:0008284 positive regulation of cell proliferation
GO:0018108 peptidyl-tyrosine phosphorylation
GO:0030335 positive regulation of cell migration
GO:0046777 protein autophosphorylation
GO:0048407 platelet-derived growth factor binding
GO:0060326 cell chemotaxis
Regulatory
For research purposes only

Applications of CD140b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 25 µg/ml
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 25 µg/ml
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Human breast cancer tissue

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Functional Assays

Immunoprecipitation

Product Specific References

References for CD140b antibody

  1. Hart, C.E. et al. (1987) Synthesis, phosphorylation, and degradation of multiple forms of the platelet-derived growth factor receptor studied using a monoclonal antibody.
    J Biol Chem. 262: 10780-5.
  2. Hart, C.E. et al. (1988) Two classes of PDGF receptor recognize different isoforms of PDGF.
    Science. 240: 1529-31.
  3. Franklin, W.A. et al. (1990) In situ distribution of the beta-subunit of platelet-derived growth factor receptor in nonneoplastic tissue and in soft tissue tumors.
    Cancer Res. 50: 6344-8.
  4. Seifert, R.A. et al. (1989) Two different subunits associate to create isoform-specific platelet-derived growth factor receptors.
    J Biol Chem. 264: 8771-8.
  5. Krane, J.F. et al. (1991) Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis.
    J Invest Dermatol. 96: 983-6.
  6. Palman, C. et al. (1992) Platelet-derived growth factor receptor (beta-subunit) immunoreactivity in soft tissue tumors.
    Lab Invest. 66: 108-15.
  7. Ascoli, V. et al. (1995) Platelet-derived growth factor receptor immunoreactivity in mesothelioma and nonneoplastic mesothelial cells in serous effusions.
    Acta Cytol. 39: 613-22.
  8. Gilbertson, D.G. et al. (2001) Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor.
    J Biol Chem. 276: 27406-14.
  9. Wang, J. et al. (1994) Cell proliferation in human soft tissue tumors correlates with platelet-derived growth factor B chain expression: an immunohistochemical and in situ hybridization study.
    Cancer Res. 54: 560-4.
  10. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
    PLoS One. 6: e17454.