CD140b antibody | PR7212

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Anti Human CD140b Antibody, clone PR7212 thumbnail image 1

Anti Human CD140b Antibody, clone PR7212 thumbnail image 2

Anti Human CD140b Antibody, clone PR7212 thumbnail image 3

Anti Human CD140b Antibody, clone PR7212 gallery image 1 — **Published customer image:**
Mouse anti Human CD140b antibody, clone PR7212 (**7460-3104**) used to evaluate PDGFRβ expression on human muscle-derived stem cells *in vitro* by flow cytometry.
**Image caption:**
Characterization and phenotype of human mdcs.
a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar = 50 μm. b. Proliferation curve of one normal human mdc preparation (pN1) and one DMD mdc preparation (pD1). The average mean population doubling time for these cells is 33.79±1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 μg/ml DAPI (blue). Scale bar = 50 μm. g. Representative FACS images showing the expression of PDGFRβ, ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green. Note that most of these markers, apart from PDGFRβ and CD56, were expressed at similar levels among cell preparations

From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011)
Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Human CD140b Antibody, clone PR7212 gallery image 2 — **Published customer image:**
Mouse anti Human CD140b antibody, clone PR7212 (**7460-3104**) used to evaluate PDGFRβ expression on human muscle-derived stem cells *in vitro* by immunofluorescence
**Image caption:**
Phenotypic differences between CD56⁺ and CD56⁻ cells.
a. Expression of MRFs and other relevant markers at the RNA level in 2 batches of total RNAs extracted from 2 separate sorts of CD56⁺ and CD56⁻ cells. b. Graph showing BrdU incorporation in CD56⁺ and CD56^- cells. c, d. Expression of desmin (green) in CD56⁺ (d) and CD56⁻ (e) cells. Nuclei were counterstained with 10 μg/ml DAPI (blue). Scale bar = 50 μm. e, f. Expression of PDGFRβ (green) in CD56⁺ (f) and CD56⁻ (g) cells. Nuclei were counterstained with 10 µg/ml DAPI (blue). Scale bar = 50 μm. g, h. Expression of BrdU (green) in CD56⁺ (h) and CD56⁻ (i) cells. Nuclei were counterstained with 10 μg/ml DAPI (blue). Quantification of BrdU⁺ cells in each population was compared with Student's t test (b). Scale bar = 100 μm.

From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011)
Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Anti Human CD140b Antibody, clone PR7212 gallery image 3 — **Published customer image:**
Mouse anti Human CD140b antibody, clone PR7212 (**7460-3104**) used to evaluate PDGFRβ expression on human muscle-derived stem cells *in vitro* by flow cytometry.
**Image caption:**
Expression of PDGFRβ (green) and CD56 (green) in 3 human mdc preparations (pN1, pD1 and pD2) by flow cytometric analysis. Control was performed using corresponding isotype control detailed in Table S1 (red). Note the expression level of these cell markers were highly variable among cell preparations.

From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011)
Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD140b

Product Type: Monoclonal Antibody
Clone: PR7212
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
7460-3104	C F IF IP P WB			0.1 mg

Mouse anti Human CD140b antibody, clone PR7212 recognizes the extracellular domain of the human PDGF receptor beta subunit. This was confirmed in binding studies using several different cell lines and by its ability to immunoprecipitate PDGF receptor beta complexed with ¹²⁵I-PDGF-BB (Hart et al. 1987). Mouse anti Human CD140b antibody, clone PR7212 does not recognize PDGF receptor alpha.

PDGF receptor beta is a member of the class III receptor tyrosine kinase family that also includes M-CSF receptor, SCF receptor and Flt-3. Binding of PDGF-BB induces receptor homodimerization or heterodimerization with PDGF receptor alpha.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Baboon
Rat
Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Human skin fibroblast cell membrane extracts.

Approx. Protein Concentrations

1.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P09619 Related reagents
Entrez Gene: PDGFRB Related reagents
GO Terms: GO:0004992 platelet activating factor receptor activity; GO:0005019 platelet-derived growth factor beta-receptor activity; GO:0005021 vascular endothelial growth factor receptor activity; GO:0005887 integral to plasma membrane; GO:0005161 platelet-derived growth factor receptor binding; GO:0005524 ATP binding; GO:0008284 positive regulation of cell proliferation; GO:0018108 peptidyl-tyrosine phosphorylation; GO:0030335 positive regulation of cell migration; GO:0046777 protein autophosphorylation; GO:0048407 platelet-derived growth factor binding; GO:0060326 cell chemotaxis
Regulatory: For research purposes only

Applications of CD140b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			25 µg/ml
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin			25 µg/ml
Immunoprecipitation
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Histology Positive Control Tissue: Human breast cancer tissue

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

Functional Assays

Immunoprecipitation

Product Specific References

References for CD140b antibody

Hart, C.E. et al. (1987) Synthesis, phosphorylation, and degradation of multiple forms of the platelet-derived growth factor receptor studied using a monoclonal antibody.
J Biol Chem. 262: 10780-5.
Hart, C.E. et al. (1988) Two classes of PDGF receptor recognize different isoforms of PDGF.
Science. 240: 1529-31.
Franklin, W.A. et al. (1990) In situ distribution of the beta-subunit of platelet-derived growth factor receptor in nonneoplastic tissue and in soft tissue tumors.
Cancer Res. 50: 6344-8.
Seifert, R.A. et al. (1989) Two different subunits associate to create isoform-specific platelet-derived growth factor receptors.
J Biol Chem. 264: 8771-8.
Krane, J.F. et al. (1991) Increased dermal expression of platelet-derived growth factor receptors in growth-activated skin wounds and psoriasis.
J Invest Dermatol. 96: 983-6.
Palman, C. et al. (1992) Platelet-derived growth factor receptor (beta-subunit) immunoreactivity in soft tissue tumors.
Lab Invest. 66: 108-15.
Ascoli, V. et al. (1995) Platelet-derived growth factor receptor immunoreactivity in mesothelioma and nonneoplastic mesothelial cells in serous effusions.
Acta Cytol. 39: 613-22.
Gilbertson, D.G. et al. (2001) Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor.
J Biol Chem. 276: 27406-14.
Wang, J. et al. (1994) Cell proliferation in human soft tissue tumors correlates with platelet-derived growth factor B chain expression: an immunohistochemical and in situ hybridization study.
Cancer Res. 54: 560-4.
Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6: e17454.

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