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CD8 antibody | 4B11

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Anti Human CD8 Antibody, clone 4B11 thumbnail image 1 Anti Human CD8 Antibody, clone 4B11 thumbnail image 2 Anti Human CD8 Antibody, clone 4B11 thumbnail image 3 Anti Human CD8 Antibody, clone 4B11 thumbnail image 4 Anti Human CD8 Antibody, clone 4B11 thumbnail image 5
Anti Human CD8 Antibody, clone 4B11 gallery image 1
Formalin fixed, paraffin processed human tonsil stained with Mouse anti Human CD8 antibody, clone 4B11. (MCA1817) Mag. 40X
Anti Human CD8 Antibody, clone 4B11 gallery image 2
Published customer image:
Mouse antiHuman CD8 antibody, clone 4B11 (MCA1817) used for the identification of CD8 expressing cells in Buruli ulcer secondary lesions by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Presence of B-cell clusters in the secondary lesions.
A: Band of CD20 positive B-cells in sections of ulcer 2 of patient 1. B–E: serial sections of nodule 3 of patient 2 with a small dense cluster of CD20 positive B-cells (B) surrounded by CD14 positive macrophages/monocytes (C) and few interspersed CD3 positive T-cells (D) from which the majority was CD8 negative (E). Higher magnification (F–I) revealed a very dense package of the B-cells.

From: Ruf M-T, Chauty A, Adeye A, Ardant M-F, Koussemou H, Johnson RC, et al. (2011)
Secondary Buruli Ulcer Skin Lesions Emerging Several Months after Completion of Chemotherapy: Paradoxical Reaction or Evidence for Immune Protection?
PLoS Negl Trop Dis 5(8): e1252.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD8 Antibody, clone 4B11 gallery image 3
Published customer image:
Mouse anti Human CD8 antibody, clone 4B11 (MCA1817) used for the detection of CD8 expressing lymphocytes in OLP tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Oral lichen planus tissue stained for CD8 expressing lymphocytes.

From: Choi YS, Kim Y, Yoon HJ, Baek KJ, Alam J, Park HK, Choi Y.
The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
Sci Rep. 2016 Jul 7;6:29186.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD8 Antibody, clone 4B11 gallery image 4
Published customer image:
Mouse anti Human CD8 antibody, clone 4B11 (MCA1817) used for the detection of CD8 expressing lymphocytes in OLP tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Presence of bacteria within epithelial cells CD3+, CD8+ and CD8− lymphocytes in OLP tissues.
Sections of OLP tissues (n = 4) were hybridized in situ for 16S rRNA and then immunostained for CD8 (H). Red arrows indicate bacteria detected within CD8+ cells; blue arrows indicate bacteria detected within CD8− lymphocytes; arrowheads indicate an immunologic synapse-like structure.

From: Choi YS, Kim Y, Yoon HJ, Baek KJ, Alam J, Park HK, Choi Y.
The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
Sci Rep. 2016 Jul 7;6:29186.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD8 Antibody, clone 4B11 gallery image 5
Published customer image:
Mouse anti Human CD8 antibody, clone 4B11 (MCA1817) used for the detection of CD8 expressing lymphocytes in OLP tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Increased infiltration of T cells correlated with the levels of bacteria in the lamina propria in OLP.
(A–D) Representative immunohistochemical detection of CD3+ cells (A), CD4+ cells (B), CD8+ cells (C), and macrophages (D) in control (n = 10) and OLP (n = 36) tissues. Each symbol in the graphs represents the mean intensity of stained signals per ROI measured in four randomly chosen fields (**P <0.01; ***P <0.001 by Mann-Whitney U test). (D) Correlation plots between the levels of bacteria in the lamina propria and the levels of CD3+ cells, CD4+ cells, CD8+ cells, or macrophages (r and P by Spearman's rank correlation test).

From: Choi YS, Kim Y, Yoon HJ, Baek KJ, Alam J, Park HK, Choi Y.
The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
Sci Rep. 2016 Jul 7;6:29186.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD8

Product Type
Monoclonal Antibody
Clone
4B11
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA1817
Datasheet
C P* WB
SDS
1 ml loader

MCA1817T
Datasheet
C P*
SDS
0.1 ml loader

Mouse anti Human CD8 antibody, clone 4B11 recognizes the human CD8 cell surface antigen, a ~32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Mouse anti Human CD8 antibody, clone 4B11 was raised based on an earlier successful strategy used to generate rabbit polyclonal antibodies against human CD8 employing the same immunizing peptide (Mason et al. 1992).

Mouse anti Human CD8 antibody, clone 4B11 has been reported as being suitable for use in Western blotting (Williamson et al. 1998) .

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Synthetic peptide derived from the carboxy terminal region of the human CD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albumin and keyhole limpet hemocyanin.
Fusion Partners
Spleen cells from immunised mice were fused with cells of a mouse p3-NS1-Ag4-1 myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P01732
P10966
Entrez Gene
CD8A
CD8B
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50
Immunohistology - Paraffin 1 1/50
Western Blotting 1/25
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Tris/EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Human Tonsil
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

Source Reference

  1. Williamson, S.L. et al. (1998) New monoclonal antibodies to the T cell antigens CD4 and CD8. Production and characterization in formalin-fixed paraffin-embedded tissue.
    Am J Pathol. 152 (6): 1421-6.

References for CD8 antibody

  1. Freysdottir, J. et al. (2007) Oral biopsies from patients with orofacial granulomatosis with histology resembling Crohn's disease have a prominent Th1 environment.
    Inflamm Bowel Dis. 13 (4): 439-45.
  2. Rees, L.E. et al. (2006) Smoking influences the immunological architecture of the human larynx.
    Clin Immunol. 118: 342-7.
  3. Choi, Y. et al. (2009) Immunohistochemical Characterization of the Human Sublingual Mucosa.
    Int J Oral Biol 34: 131-5.
  4. Kim, Y.C. et al. (2010) Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis.
    Oral Dis. 16: 375-81.
  5. Ruf, M.T. et al. (2011) Secondary buruli ulcer skin lesions emerging several months after completion of chemotherapy: paradoxical reaction or evidence for immune protection?
    PLoS Negl Trop Dis. 5: e1252.
  6. Matsuura, E. et al. (2017) Dynamic acquisition of HTLV-1 tax protein by mononuclear phagocytes: Role in neurologic disease.
    J Neuroimmunol. 304: 43-50.
  7. Weber, B. et al. (2017) Distinct interferon-gamma and interleukin-9 expression in cutaneous and oral lichen planus.
    J Eur Acad Dermatol Venereol. 31 (5): 880-6.
  8. Choi, Y.S. et al. (2016) The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
    Sci Rep. 6: 29186.
  9. Sabolek, M.T.. et al. (2019) Communication of CD8+ T cells with mononuclear phagocytes in multiple sclerosis.
    Ann Clin Transl Neurol. 6 (7): 1151-64.

Further Reading

  1. Mason, D.Y. et al. (1992) Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.
    J Clin Pathol. 45 (12): 1084-8.
  2. Brunati, S. et al. (1987) Production and characterization of a rabbit antiserum to the mouse CD8 antigenic complex by immunization with a synthetic peptide.
    J Immunol Methods. 96 (1): 97-105.

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