CD8 antibody | 4B11

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Mouse anti Human CD8

Product Type
Monoclonal Antibody
Clone
4B11
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1817T C P* 0.1 ml
MCA1817 C P* WB 1 ml
Mouse anti Human CD8 antibody, clone 4B11 recognizes the human CD8 cell surface antigen, a ~32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Mouse anti Human CD8 antibody, clone 4B11 was raised based on an earlier successful strategy used to generate rabbit polyclonal antibodies against human CD8 employing the same immunizing peptide (Mason et al. 1992).

Mouse anti Human CD8 antibody, clone 4B11 has been reported as being suitable for use in Western blotting (Williamson et al. 1998) .

Product Details

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Synthetic peptide derived from the carboxy terminal region of the human CD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albumin and keyhole limpet hemocyanin.
Fusion Partners
Spleen cells from immunised mice were fused with cells of a mouse p3-NS1-Ag4-1 myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P01732 Related reagents
P10966 Related reagents
Entrez Gene
CD8A Related reagents
CD8B Related reagents
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50
Immunohistology - Paraffin 1 1/50
Western Blotting 1/25
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Tris/EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Human Tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

Source Reference

  1. Williamson, S.L. et al. (1998) New monoclonal antibodies to the T cell antigens CD4 and CD8. Production and characterization in formalin-fixed paraffin-embedded tissue.
    Am J Pathol. 152 (6): 1421-6.

References for CD8 antibody

  1. Freysdottir, J. et al. (2007) Oral biopsies from patients with orofacial granulomatosis with histology resembling Crohn's disease have a prominent Th1 environment.
    Inflamm Bowel Dis. 13 (4): 439-45.
  2. Rees, L.E. et al. (2006) Smoking influences the immunological architecture of the human larynx.
    Clin Immunol. 118: 342-7.
  3. Choi, Y. et al. (2009) Immunohistochemical Characterization of the Human Sublingual Mucosa.
    Int. J. Oral. Biol. 34: 131-135.
  4. Kim, Y.C. et al. (2010) Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis.
    Oral Dis. 16: 375-81.
  5. Ruf, M.T. et al. (2011) Secondary buruli ulcer skin lesions emerging several months after completion of chemotherapy: paradoxical reaction or evidence for immune protection?
    PLoS Negl Trop Dis. 5: e1252.
  6. Matsuura, E. et al. (2016) Dynamic acquisition of HTLV-1 tax protein by mononuclear phagocytes: Role in neurologic disease
    Journal of Neuroimmunology. Oct 3 [Epub ahead of print]
  7. Weber, B. et al. (2016) Distinct interferon-gamma and interleukin-9 expression in cutaneous and oral lichen planus.
    J Eur Acad Dermatol Venereol. Oct 1 [Epub ahead of print]
  8. Choi, Y.S. et al. (2016) The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
    Sci Rep. 6: 29186.

Further Reading

  1. Mason, D.Y. et al. (1992) Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.
    J Clin Pathol. 45 (12): 1084-8.
  2. Brunati, S. et al. (1987) Production and characterization of a rabbit antiserum to the mouse CD8 antigenic complex by immunization with a synthetic peptide.
    J Immunol Methods. 96 (1): 97-105.