CD8 antibody | 4B11
Mouse antiHuman CD8 antibody, clone 4B11 (MCA1817) used for the identification of CD8 expressing cells in Buruli ulcer secondary lesions by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Presence of B-cell clusters in the secondary lesions.
A: Band of CD20 positive B-cells in sections of ulcer 2 of patient 1. B–E: serial sections of nodule 3 of patient 2 with a small dense cluster of CD20 positive B-cells (B) surrounded by CD14 positive macrophages/monocytes (C) and few interspersed CD3 positive T-cells (D) from which the majority was CD8 negative (E). Higher magnification (F–I) revealed a very dense package of the B-cells.
From: Ruf M-T, Chauty A, Adeye A, Ardant M-F, Koussemou H, Johnson RC, et al. (2011)
Secondary Buruli Ulcer Skin Lesions Emerging Several Months after Completion of Chemotherapy: Paradoxical Reaction or Evidence for Immune Protection?
PLoS Negl Trop Dis 5(8): e1252.
Mouse anti Human CD8 antibody, clone 4B11 (MCA1817) used for the detection of CD8 expressing lymphocytes in OLP tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Oral lichen planus tissue stained for CD8 expressing lymphocytes.
From: Choi YS, Kim Y, Yoon HJ, Baek KJ, Alam J, Park HK, Choi Y.
The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
Sci Rep. 2016 Jul 7;6:29186.
Mouse anti Human CD8 antibody, clone 4B11 (MCA1817) used for the detection of CD8 expressing lymphocytes in OLP tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Presence of bacteria within epithelial cells CD3+, CD8+ and CD8− lymphocytes in OLP tissues.
Sections of OLP tissues (n = 4) were hybridized in situ for 16S rRNA and then immunostained for CD8 (H). Red arrows indicate bacteria detected within CD8+ cells; blue arrows indicate bacteria detected within CD8− lymphocytes; arrowheads indicate an immunologic synapse-like structure.
From: Choi YS, Kim Y, Yoon HJ, Baek KJ, Alam J, Park HK, Choi Y.
The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
Sci Rep. 2016 Jul 7;6:29186.
Mouse anti Human CD8 antibody, clone 4B11 (MCA1817) used for the detection of CD8 expressing lymphocytes in OLP tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Increased infiltration of T cells correlated with the levels of bacteria in the lamina propria in OLP.
(A–D) Representative immunohistochemical detection of CD3+ cells (A), CD4+ cells (B), CD8+ cells (C), and macrophages (D) in control (n = 10) and OLP (n = 36) tissues. Each symbol in the graphs represents the mean intensity of stained signals per ROI measured in four randomly chosen fields (**P <0.01; ***P <0.001 by Mann-Whitney U test). (D) Correlation plots between the levels of bacteria in the lamina propria and the levels of CD3+ cells, CD4+ cells, CD8+ cells, or macrophages (r and P by Spearman's rank correlation test).
From: Choi YS, Kim Y, Yoon HJ, Baek KJ, Alam J, Park HK, Choi Y.
The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
Sci Rep. 2016 Jul 7;6:29186.
Mouse anti Human CD8 antibody, clone 4B11 recognizes the human CD8 cell surface antigen, a ~32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Mouse anti Human CD8 antibody, clone 4B11 was raised based on an earlier successful strategy used to generate rabbit polyclonal antibodies against human CD8 employing the same immunizing peptide (Mason et al. 1992).
Mouse anti Human CD8 antibody, clone 4B11 has been reported as being suitable for use in Western blotting (Williamson et al. 1998) .
Mouse anti Human CD8 antibody, clone 4B11 has been reported as being suitable for use in Western blotting (Williamson et al. 1998) .
Product Details
- Target Species
- Human
- Product Form
- Tissue Culture Supernatant - liquid
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Synthetic peptide derived from the carboxy terminal region of the human CD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albumin and keyhole limpet hemocyanin.
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of a mouse p3-NS1-Ag4-1 myeloma cell line.
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
More Information
- UniProt
- P01732 Related reagents
- P10966 Related reagents
- Entrez Gene
- CD8A Related reagents
- CD8B Related reagents
- GO Terms
- GO:0005576 extracellular region
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0015026 coreceptor activity
- GO:0019882 antigen processing and presentation
- GO:0042101 T cell receptor complex
- GO:0042288 MHC class I protein binding
- GO:0050776 regulation of immune response
- Regulatory
- For research purposes only
Applications of CD8 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/50 | ||
| Immunohistology - Paraffin 1 | 1/50 | ||
| Western Blotting | 1/25 |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Tris/EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human Tonsil
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
Source Reference
-
Williamson, S.L. et al. (1998) New monoclonal antibodies to the T cell antigens CD4 and CD8. Production and characterization in formalin-fixed paraffin-embedded tissue.
Am J Pathol. 152 (6): 1421-6.
References for CD8 antibody
-
Freysdottir, J. et al. (2007) Oral biopsies from patients with orofacial granulomatosis with histology resembling Crohn's disease have a prominent Th1 environment.
Inflamm Bowel Dis. 13 (4): 439-45. -
Rees, L.E. et al. (2006) Smoking influences the immunological architecture of the human larynx.
Clin Immunol. 118: 342-7. -
Choi, Y. et al. (2009) Immunohistochemical Characterization of the Human Sublingual Mucosa.
Int. J. Oral. Biol. 34: 131-135. -
Kim, Y.C. et al. (2010) Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis.
Oral Dis. 16: 375-81. -
Ruf, M.T. et al. (2011) Secondary buruli ulcer skin lesions emerging several months after completion of chemotherapy: paradoxical reaction or evidence for immune protection?
PLoS Negl Trop Dis. 5: e1252. -
Matsuura, E. et al. (2016) Dynamic acquisition of HTLV-1 tax protein by mononuclear phagocytes: Role in neurologic disease
Journal of Neuroimmunology. Oct 3 [Epub ahead of print] -
Weber, B. et al. (2016) Distinct interferon-gamma and interleukin-9 expression in cutaneous and oral lichen planus.
J Eur Acad Dermatol Venereol. Oct 1 [Epub ahead of print] -
Choi, Y.S. et al. (2016) The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
Sci Rep. 6: 29186.
Further Reading
-
Mason, D.Y. et al. (1992) Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.
J Clin Pathol. 45 (12): 1084-8. -
Brunati, S. et al. (1987) Production and characterization of a rabbit antiserum to the mouse CD8 antigenic complex by immunization with a synthetic peptide.
J Immunol Methods. 96 (1): 97-105.
