CD8 antibody | 4B11

Mouse anti Human CD8

Product Type
Monoclonal Antibody
Clone
4B11
Isotype
IgG2b
Specificity
CD8

Product Code Applications Pack Size List Price Your Price Qty
MCA1817
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C P * WB 1 ml loader
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loader
MCA1817T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C P * 0.1 ml loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD8 antibody, clone 4B11 recognizes the human CD8 cell surface antigen, a ~32 kDa glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. Mouse anti Human CD8 antibody, clone 4B11 was raised based on an earlier successful strategy used to generate rabbit polyclonal antibodies against human CD8 employing the same immunizing peptide (Mason et al. 1992).

Mouse anti Human CD8 antibody, clone 4B11 has been reported as being suitable for use in Western blotting (Williamson et al. 1998) .

Target Species
Human
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
Pack Size: 1 ml
0.09%Sodium Azide
Pack Size: 0.1 ml
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide derived from the carboxy terminal region of the human CD8 alpha chain coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV. The peptide was coupled to bovine serum albumin and keyhole limpet hemocyanin.
Fusion Partners
Spleen cells from immunised mice were fused with cells of a mouse p3-NS1-Ag4-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50
Immunohistology - Paraffin 1 1/50
Western Blotting 1/25
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Tris/EDTA buffer pH 8.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue
Human Tonsil

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Source Reference

  1. Williamson, S.L. et al. (1998) New monoclonal antibodies to the T cell antigens CD4 and CD8. Production and characterization in formalin-fixed paraffin-embedded tissue.
    Am J Pathol. 152 (6): 1421-6.

References for CD8 antibody

  1. Freysdottir, J. et al. (2007) Oral biopsies from patients with orofacial granulomatosis with histology resembling Crohn's disease have a prominent Th1 environment.
    Inflamm Bowel Dis. 13 (4): 439-45.
  2. Rees, L.E. et al. (2006) Smoking influences the immunological architecture of the human larynx.
    Clin Immunol. 118: 342-7.
  3. Choi, Y. et al. (2009) Immunohistochemical Characterization of the Human Sublingual Mucosa.
    Int J Oral Biol 34: 131-5.
  4. Kim, Y.C. et al. (2010) Presence of Porphyromonas gingivalis and plasma cell dominance in gingival tissues with periodontitis.
    Oral Dis. 16: 375-81.
  5. Ruf, M.T. et al. (2011) Secondary buruli ulcer skin lesions emerging several months after completion of chemotherapy: paradoxical reaction or evidence for immune protection?
    PLoS Negl Trop Dis. 5: e1252.
  6. Matsuura, E. et al. (2017) Dynamic acquisition of HTLV-1 tax protein by mononuclear phagocytes: Role in neurologic disease.
    J Neuroimmunol. 304: 43-50.
  7. Weber, B. et al. (2017) Distinct interferon-gamma and interleukin-9 expression in cutaneous and oral lichen planus.
    J Eur Acad Dermatol Venereol. 31 (5): 880-6.
  8. Choi, Y.S. et al. (2016) The presence of bacteria within tissue provides insights into the pathogenesis of oral lichen planus.
    Sci Rep. 6: 29186.
  9. View The Latest Product References
  10. Sabolek, M.T.. et al. (2019) Communication of CD8+ T cells with mononuclear phagocytes in multiple sclerosis.
    Ann Clin Transl Neurol. 6 (7): 1151-64.
  11. Kim, H.D. et al. (2021) Implication of CD69+ CD103+ tissue-resident-like CD8+ T cells as a potential immunotherapeutic target for cholangiocarcinoma.
    Liver Int. 41 (4): 764-76.

Further Reading

  1. Mason, D.Y. et al. (1992) Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence.
    J Clin Pathol. 45 (12): 1084-8.
  2. Brunati, S. et al. (1987) Production and characterization of a rabbit antiserum to the mouse CD8 antigenic complex by immunization with a synthetic peptide.
    J Immunol Methods. 96 (1): 97-105.

RRID
AB_322868
UniProt
P01732
P10966
Entrez Gene
CD8A
CD8B
GO Terms
GO:0005576 extracellular region
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0005887 integral to plasma membrane
GO:0015026 coreceptor activity
GO:0019882 antigen processing and presentation
GO:0042101 T cell receptor complex
GO:0042288 MHC class I protein binding
GO:0050776 regulation of immune response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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