IgG antibody
Biotinylated Goat anti Rat IgG antibody (STAR131B) used to detect bound Rat anti Murine F4/80 antibody, clone A3-1 (MCA497GA) on formalin fixed, paraffin embedded tissue sections by immunohistochemistry.
Image caption:
Tubulointerstitial inflammation and loss of peritubular capillaries in CKD kidneys.
Kidney sections of control and CKD mice were stained for macrophages (A), neutrophils (B), and vascular endothelium (C). Shown are representative immunohistochemical stainings of control and CKD kidneys and quantitative analyses of each staining categorized for renal cortex and outer medulla. Black bars represent control (n = 15) and dashed bars CKD kidneys (n = 16). Scale bars, 50μm. ** p<0.01.
From: Schley G, Jordan J, Ellmann S, Rosen S, Eckardt K-U, Uder M, et al. (2018)
Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS ONE 13(7): e0200259.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Goat anti Rat IgG antibody recognizes rat IgG. This antibody has been cross absorbed against pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Antiserum Preparation
- Antisera to rat IgG were raised by repeated immunisation of goats with purified antigen. Purified IgG prepared by affinity chromatography
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Rat IgG
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- Please see label for expiry date.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P20761 Related reagents
- P20762 Related reagents
- P20760 Related reagents
- P20759 Related reagents
- Entrez Gene
- Ighg Related reagents
- LOC679045 Related reagents
- LOC362795 Related reagents
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2000 | 1/4000 | |
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting | |||
| ELISA | 1/5000 | 1/20000 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for IgG antibody
-
Monticone, S. et al. (2015) Immunohistochemical, genetic and clinical characterization of sporadic aldosterone-producing adenomas.
Mol Cell Endocrinol. pii: S0303-7207(15)00223-3. -
Schley, G. et al. (2018) Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS One. 13 (7): e0200259.
