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IgG antibody

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Anti Rat IgG (Mouse Adsorbed) Ab - AP gallery image 1
Published customer image:
Biotinylated Goat anti Rat IgG antibody (STAR131B) used to detect bound Rat anti Murine F4/80 antibody, clone A3-1 (MCA497GA) on formalin fixed, paraffin embedded tissue sections by immunohistochemistry.
Image caption:
Tubulointerstitial inflammation and loss of peritubular capillaries in CKD kidneys.
Kidney sections of control and CKD mice were stained for macrophages (A), neutrophils (B), and vascular endothelium (C). Shown are representative immunohistochemical stainings of control and CKD kidneys and quantitative analyses of each staining categorized for renal cortex and outer medulla. Black bars represent control (n = 15) and dashed bars CKD kidneys (n = 16). Scale bars, 50μm. ** p<0.01.

From: Schley G, Jordan J, Ellmann S, Rosen S, Eckardt K-U, Uder M, et al. (2018)
Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
PLoS ONE 13(7): e0200259.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

Goat anti Rat IgG:Biotin (Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR131A
Datasheet
C E P WB
SDS
1 ml loader

STAR131B
Datasheet
C E IF P WB
SDS
0.5 mg loader

Goat anti Rat IgG antibody recognizes rat IgG. This antibody has been cross absorbed against pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Antiserum Preparation
Antisera to rat IgG were raised by repeated immunisation of goats with purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
50% Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Rat IgG
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.
Guarantee
12 months from date of despatch

More Information

UniProt
P20761
P20762
P20760
P20759
Entrez Gene
Ighg
LOC679045
LOC362795
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/4000
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
ELISA 1/5000 1/20000
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Product Specific References

References for IgG antibody

  1. Monticone, S. et al. (2015) Immunohistochemical, genetic and clinical characterization of sporadic aldosterone-producing adenomas.
    Mol Cell Endocrinol. pii: S0303-7207(15)00223-3.
  2. Schley, G. et al. (2018) Multiparametric magnetic resonance imaging of experimental chronic kidney disease: A quantitative correlation study with histology.
    PLoS One. 13 (7): e0200259.
  3. El Amki, M. et al. (2018) Improved Reperfusion and Vasculoprotection by the Poly(ADP-Ribose)Polymerase Inhibitor PJ34 After Stroke and Thrombolysis in Mice.
    Mol Neurobiol. 55 (12): 9156-9168.

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