CD3 antibody | CD3-12

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Rat anti Human CD3:Alexa Fluor®488

CD3 antibody, clone CD3-12, recognizes human CD3e, and has a very broad species crossreactivity for the CD3 marker. CD3 is a multimeric protein complex composed of four distinct polypeptide chains that associate with the T cell receptor (TCR).

Rat anti Human CD3:Alexa Fluor®647

CD3 antibody, clone CD3-12, recognizes human CD3e, and has a very broad species crossreactivity for the CD3 marker. CD3 is a multimeric protein complex composed of four distinct polypeptide chains that associate with the T cell receptor (TCR).

Rat anti Human CD3:FITC

CD3 antibody, clone CD3-12, recognizes human CD3e, and has a very broad species crossreactivity for the CD3 marker. CD3 is a multimeric protein complex composed of four distinct polypeptide chains that associate with the T cell receptor (TCR).

Rat anti Human CD3:Pacific Blue®

CD3 antibody, clone CD3-12, recognizes human CD3e, and has a very broad species crossreactivity for the CD3 marker. CD3 is a multimeric protein complex composed of four distinct polypeptide chains that associate with the T cell receptor (TCR).

Rat anti Human CD3

CD3 antibody, clone CD3-12, recognizes human CD3e, and has a very broad species crossreactivity for the CD3 marker. CD3 is a multimeric protein complex composed of four distinct polypeptide chains that associate with the T cell receptor (TCR).

Product Type
Monoclonal Antibody
Clone
CD3-12
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1477A488 F* 100 Tests/1ml
MCA1477A647 F* 100 Tests/1ml
MCA1477FT F* 25 µg
MCA1477F F* 0.1 mg
MCA1477PB F* 100 Tests/1ml
MCA1477T C F* IF P* 25 µg
MCA1477 C F* IF P* WB 0.2 mg
Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker.

As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Dog
Horse
Rhesus Monkey
Pig
Chicken
Mouse
Duck
Koala
Harbour Porpoise
Alpaca
Cynomolgus monkey
Spotted Hyena
Sea Lion
Cat
Amazon Parrot
Mammals Expected from Sequence
Birds Expected from Sequence
Raccoon
Great horned owl (Bubo virginianus)
Bullfrog
Xenopus
Amphibia Expected from Sequence
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG - conjugated to Alexa Fluor®647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC )
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07766 Related reagents
Entrez Gene
CD3E Related reagents
GO Terms
GO:0004888 transmembrane receptor activity
GO:0005057 receptor signaling protein activity
GO:0005887 integral to plasma membrane
GO:0007172 signal complex assembly
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0009897 external side of plasma membrane
GO:0017124 SH3 domain binding
GO:0019901 protein kinase binding
GO:0030159 receptor signaling complex scaffold activity
GO:0031295 T cell costimulation
GO:0042608 T cell receptor binding
GO:0046982 protein heterodimerization activity
GO:0050852 T cell receptor signaling pathway
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/5 1/10
Flow Cytometry 1 Neat
Flow Cytometry 1 1/5 1/10
Flow Cytometry 1 1/5 1/10
Flow Cytometry 1 1/50 1/100
Immunofluorescence
Immunohistology - Frozen 1/100
Immunohistology - Paraffin 2 1/100
Western Blotting
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm™ (BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm™ (BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm™ (BUF09) for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems with appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Tonsil

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control:Alexa Fluor® 488 MCA6004A488 100 Tests/1ml F
Rat IgG1 Negative Control:Alexa Fluor® 647 MCA6004A647 100 Tests/1ml F
Rat IgG1 Negative Control:FITC MCA6004F 0.1 mg F
Rat IgG1 Negative Control:Pacific Blue® MCA6004PB 100 Tests/1ml F
Rat IgG1 Negative Control MCA6004GA 0.1 mg F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for CD3 antibody

  1. Jones, M. et al. (1993) Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
    J Immunol. 150 (12): 5429-35.
  2. Shulga-Morskaya, S. et al. (2004) B cell-activating factor belonging to the TNF family acts through separate receptors to support B cell survival and T cell-independent antibody formation.
    J Immunol. 173 (4): 2331-41.
  3. Kapturczak, M.H. et al. (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse.
    Am J Pathol. 165 (3): 1045-53.
  4. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies.
    Dev Comp Immunol. 29 (8): 733-48.
  5. Patole, P.S. et al. (2006) Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice.
    Nephrol Dial Transplant 21 (11): 3062-73.
  6. Foryst-Ludwig, A. et al. (2010) PPARgamma activation attenuates T-lymphocyte-dependent inflammation of adipose tissue and development of insulin resistance in obese mice.
    Cardiovasc Diabetol. 9: 64.
  7. Osorio, Y. et al. (2011) Identification of small molecule lead compounds for visceral leishmaniasis using a novel ex vivo splenic explant model system.
    PLoS Negl Trop Dis. 5:e962.
  8. Flatz, L. et al (2011) T cell-dependence of Lassa fever pathogenesis.
    PLoS Pathog. 6: e1000836.
  9. Gendronneau, G. et al. (2010) Influence of Hoxa5 on p53 tumorigenic outcome in mice.
    Am J Pathol. 176: 995-1005.
  10. Herrmann, I. et al. (2006) Streptococcus pneumoniae Infection aggravates experimental autoimmune encephalomyelitis via Toll-like receptor 2.
    Infect Immun. 74: 4841-8.
  11. Ruf, M.T. et al. (2012) Chemotherapy-Associated Changes of Histopathological Features of Mycobacterium ulcerans Lesions in a Buruli Ulcer Mouse Model.
    Antimicrob Agents Chemother. 56: 687-96.
  12. Roy, M. et al. (2012) CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
    J Neuroinflammation. 9: 18.
  13. Campuzano, O. et al. (2012) Arrhythmogenic right ventricular cardiomyopathy: severe structural alterations are associated with inflammation.
    J Clin Pathol. 65 (12): 1077-83.
  14. Lau, Q. et al. (2012) Expression and in vitro upregulation of MHCII in koala lymphocytes.
    Vet Immunol Immunopathol. 147: 35-43.
  15. Beineke, A. et al. (2007) Phenotypical characterization of changes in thymus and spleen associated with lymphoid depletion in free-ranging harbor porpoises (Phocoena phocoena).
    Vet Immunol Immunopathol. 117: 254-65.
  16. Pusterla, N. et al. (2006) Multicentric T-cell lymphosarcoma in an alpaca.
    Vet J. 171: 181-5.
  17. Fuller, C.L. et al. (2003) In situ study of abundant expression of proinflammatory chemokines and cytokines in pulmonary granulomas that develop in cynomolgus macaques experimentally infected with Mycobacterium tuberculosis.
    Infect Immun. 71: 7023-34.
  18. Singleton, C.L. et al. (2007) Diagnosis and treatment of chronic T-lymphocytic leukemia in a spotted hyena (Crocuta crocuta).
    J Zoo Wildl Med. 38: 488-91.
  19. Colegrove, K.M. et al. (2010) Polyomavirus infection in a free-ranging California sea lion (Zalophus californianus) with intestinal T-cell lymphoma.
    J Vet Diagn Invest. 22: 628-32.
  20. Steinberg, J.D. and Keating, J.H. (2008) What is your diagnosis? Cervical mass in a cat.
    Vet Clin Pathol. 37: 323-7.
  21. Osofsky, A. et al. (2011) T-cell chronic lymphocytic leukemia in a double yellow-headed Amazon parrot (Amazona ochrocephala oratrix).
    J Avian Med Surg. 25: 286-94.
  22. Giannitti, F. et al. (2014) Temporal and geographic clustering of polyomavirus-associated olfactory tumors in 10 free-ranging raccoons (Procyon lotor).
    Vet Pathol. 51 (4): 832-45.
  23. Malka, S. et al. (2008) Disseminated lymphoma of presumptive T-cell origin in a great horned owl (Bubo virginianus).
    J Avian Med Surg. 22: 226-33.
  24. Bricker, N.K. et al. (2012) Cytochemical and immunocytochemical characterization of blood cells and immunohistochemical analysis of spleen cells from 2 species of frog, Rana (Aquarana) catesbeiana and Xenopus laevis.
    Vet Clin Pathol. 41: 353-61.
  25. de Winde, C.M. et al. (2015) Multispectral imaging reveals the tissue distribution of tetraspanins in human lymphoid organs.
    Histochem Cell Biol. 144 (2): 133-46.
  26. Dewals B.G., et al. (2011) Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4- T cells expressing a cytotoxic phenotype in both lymphoid and non-lymphoid tissues
    Vet Res. 42:95
  27. Muljono, A. et al. (2009) Primary cutaneous lymphoblastic lymphoma in children: series of eight cases with review of the literature.
    Pathology. 41 (3): 223-8.
  28. Sommer, A. et al. (2016) Infiltrating T lymphocytes reduce myeloid phagocytosis activity in synucleinopathy model.
    J Neuroinflammation 13 (1): 174.
  29. Velu, V. et al. (2016) Induction of Th1-Biased T Follicular Helper (Tfh) Cells in Lymphoid Tissues during Chronic Simian Immunodeficiency Virus Infection Defines Functionally Distinct Germinal Center Tfh Cells.
    J Immunol. Aug 1. pii: 1600143. [Epub ahead of print]
  30. Wen, J. et al. (2015) TNF-like weak inducer of apoptosis promotes blood brain barrier disruption and increases neuronal cell death in MRL/lpr mice.
    J Autoimmun. 60: 40-50.
  31. Sample, S.J. et al. (2017) Radiographic and magnetic resonance imaging predicts severity of cruciate ligament fiber damage and synovitis in dogs with cranial cruciate ligament rupture.
    PLoS One. 12 (6): e0178086.
  32. Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
    J Clin Invest. 125 (11): 4281-94.
  33. Kallikourdis, M. et al. (2017) T cell costimulation blockade blunts pressure overload-induced heart failure.
    Nat Commun. 8: 14680.
  34. Bonnefont-Rebeix, C. et al. (2016) Characterization of a novel canine T-cell line established from a spontaneously occurring aggressive T-cell lymphoma with large granular cell morphology.
    Immunobiology. 221 (1): 12-22.
  35. Montes-Cobos, E. et al. (2017) Targeted delivery of glucocorticoids to macrophages in a mouse model of multiple sclerosis using inorganic-organic hybrid nanoparticles.
    J Control Release. 245: 157-169.
  36. Bartlett SL et al. (2010) Intestinal lymphoma of granular lymphocytes in a fisher (Martes pennanti) and a Eurasian otter (Lutra lutra).
    J Zoo Wildl Med. 41 (2): 309-15.
  37. Houser, K.V. et al. (2017) Enhanced inflammation in New Zealand white rabbits when MERS-CoV reinfection occurs in the absence of neutralizing antibody.
    PLoS Pathog. 13 (8): e1006565.
  38. Sparger, E.E. et al. (2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.
    Vet Immunol Immunopathol. 202: 52-62.
  39. Palomo, J. et al. (2018) The severity of imiquimod-induced mouse skin inflammation is independent of endogenous IL-38 expression.
    PLoS One. 13 (3): e0194667.
  40. Declue, A.E. et al. (2018) Identification of immunologic and clinical characteristics that predict inflammatory response to C. Novyi-NT bacteriolytic immunotherapy.
    BMC Vet Res. 14 (1): 119.
  41. DaSilva, A.V.A. et al. (2018) Morphophysiological changes in the splenic extracellular matrix of Leishmania infantum-naturally infected dogs is associated with alterations in lymphoid niches and the CD4+ T cell frequency in spleens.
    PLoS Negl Trop Dis. 12 (4): e0006445.
  42. Withers, S.S. et al. (2018) Multi-color flow cytometry for evaluating age-related changes in memory lymphocyte subsets in dogs.
    Dev Comp Immunol. 87: 64-74.

Further Reading

  1. Alterio de Goss, M. et al. (1998) Control of cytomegalovirus in bone marrow transplantation chimeras lacking the prevailing antigen-presenting molecule in recipient tissues rests primarily on recipient-derived CD8 T cells.
    J Virol. 72 (10): 7733-44.
  2. Burudi, E.M. et al. (2002) Regulation of indoleamine 2,3-dioxygenase expression in simian immunodeficiency virus-infected monkey brains.
    J Virol. 76 (23): 12233-41.
  3. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

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