CD56 antibody | ERIC-1
Mouse anti Human CD56 antibody, clone ERIC-1 (MCA591) used to label CD56 expressing cells in human HIV-associated myosistis samples by immunohistochemistry of cryosections.
Image caption:
Severity of histopathological changes p62, CD56, MHCneo.
Severity levels of histopathological changes: 2 (moderate), 3 (severe). P62 staining shows autophagy with autophagic structures concentrated within the vacuoles in a few fibres (severity level 2, a3) and in multiple fibres (severity level 3, a4). CD56 and neonatal MHC staining show regenerating fibres with increasing numbers for each level of severity (b3-4, c3-4).
From: Vogt S, Kleefeld F, Preusse C, Arendt G, Bieneck S, Brunn A, Deckert M, Englert B, Goebel HH, Masuhr A, Neuen-Jacob E, Kornblum C, Reimann J, Montagnese F, Schoser B, Stenzel W, Hahn K.
Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis.
J Neurol. 2023 Sep;270(9):4434-43.
doi: 10.1007/s00415-023-11779-y.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD56 antibody, clone ERIC-1 (MCA591) used to label neuronal cell presynaptin membrane adhesions by immunofluorescence.
Image caption:
NMJs in skeletal muscles of anti-AChR-Ab-positive MG and healthy controls:
b. Double immunofluorescence staining of intercostal muscle biopsies from anti-AChR-Ab-positive MG patients (original magnification × 400). NMJs are indicated by arrows. The same exposure time was used for all images. Scale bars are 100 µm. CD56 is a neuronal cell adhesion molecule on the presynaptic membrane. Overlapping of CD56 and ITIH3 staining showed that ITIH3 is mainly localized at the postsynaptic membrane. Desmin is an intermediate filament-associated protein known to be enriched at the postsynaptic membrane of NMJs. Double immunofluorescence of ITIH3 and desmin showed an overlap of both markers, confirming the postsynaptic localization of ITIH3. Plectin is an intermediate filament-associated protein that links cytoskeletal components. It is highly expressed at the postsynaptic membrane and supports its junctional folds. Double staining of ITIH3 and plectin showed colocalization of both proteins. Anti-AChR-Ab anti-acetylcholine receptor antibody, DDA data-dependent acquisition, DES desmin, HC healthy control, IHC immunohistochemistry, ITIH3 inter-alpha-trypsin inhibitor heavy chain H3, LC liquid chromatography, MG myasthenia gravis, MS/MS tandem mass spectrometry, NMJ neuromuscular junction, NSE neuron-specific enolase, PLEC plectin
From: Schroeter CB, Nelke C, Stascheit F, Huntemann N, Preusse C, Dobelmann V, Theissen L, Pawlitzki M, Räuber S, Willison A, Vogelsang A, Marina AD, Hartung HP, Melzer N, Konen FF, Skripuletz T, Hentschel A, König S, Schweizer M, Stühler K, Poschmann G, Roos A, Stenzel W, Meisel A, Meuth SG, Ruck T.
Inter-alpha-trypsin inhibitor heavy chain H3 is a potential biomarker for disease activity in myasthenia gravis.
Acta Neuropathol. 2024 Jun 18;147(1):102.
doi: 10.1007/s00401-024-02754-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
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- Product Type
- Monoclonal Antibody
- Clone
- ERIC-1
- Isotype
- IgG1
- Specificity
- CD56
Antibody Quality and Performance Guarantee
| Mouse anti Human CD56 antibody, clone ERIC-1 recognizes N-CAM expressed on developing and adult neuroectodermal tissues in humans. Neuroectodermal tumours also stain including Glioma, ependymoma, neuroblastoma, medulloblastoma, retinoblastoma and teratoma. Oat cell carcinoma and Wilms tumour are also highly reactive. Mouse anti Human CD56 antibody, clone ERIC-1 will react on Natural Killer cells and recognizes 140, 180 and 120 kDa NCAM isoforms. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Human retinoblastoma tumour cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the P3/X63.Ag8 mouse myeloma line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
80ng/ml | |
| Flow Cytometry | |||
| Immunoblotting | |
||
| Immunohistology - Frozen | |
1/50 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Neuroblastoma
References for CD56 antibody
-
Bourne, S.P. et al. (1991) A monoclonal antibody (ERIC-1), raised against retinoblastoma, that recognizes the neural cell adhesion molecule (NCAM) expressed on brain and tumours arising from the neuroectoderm.
J Neurooncol. 10 (2): 111-9. -
Criel, A. et al. (1997) Further characterization of morphologically defined typical and atypical CLL: a clinical, immunophenotypic, cytogenetic and prognostic study on 390 cases.
Br J Haematol. 97 (2): 383-91. -
Cameron, A.L. et al. (2002) Natural killer and natural killer-T cells in psoriasis.
Arch Dermatol Res. 294 (8): 363-9. -
Quenby, S. et al. (2005) Prednisolone reduces preconceptual endometrial natural killer cells in women with recurrent miscarriage.
Fertil Steril. 84 (4): 980-4. -
McIntosh K et al. (2006) The immunogenicity of human adipose-derived cells: temporal changes in vitro.
Stem Cells. 24 (5): 1246-53. -
Whitworth, M.K. et al. (2007) Cervical leukocyte sub-populations in idiopathic preterm labour.
J Reprod Immunol. 75: 48-55. -
Preuße, C. et al. (2012) Immune-mediated necrotizing myopathy is characterized by a specific Th1-M1 polarized immune profile.
Am J Pathol. 181 (6): 2161-71. -
Debeer, S. et al. (2013) Comparative histology and immunohistochemistry of porcine versus human skin.
Eur J Dermatol. 23 (4): 456-66.
View The Latest Product References
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Salvatore, G. et al. (2015) Human monocyte-derived dendritic cells turn into foamy dendritic cells with IL-17A.
J Lipid Res. 56 (6): 1110-22. -
Preuße, C. et al. (2016) Differential roles of hypoxia and innate immunity in juvenile and adult dermatomyositis.
Acta Neuropathol Commun. 4 (1): 45. -
Allenbach, Y. et al. (2016) Dermatomyositis With or Without Anti-Melanoma Differentiation-Associated Gene 5 Antibodies: Common Interferon Signature but Distinct NOS2 Expression.
Am J Pathol. 186 (3): 691-700. -
Siegert, E. et al. (2021) Systemic sclerosis-associated myositis features minimal inflammation and characteristic capillary pathology.
Acta Neuropathol. 141 (6): 917-27. -
Meinhardt, J. et al. (2021) Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19.
Nat Neurosci. 24 (2): 168-75. -
Vogt, S. et al. (2023) Morphological and molecular comparison of HIV-associated and sporadic inclusion body myositis.
J Neurol. 270 (9): 4434-43. -
Englert, B. et al. (2024) "Amyopathic" MDA5-positive dermatomyositis with severe lung involvement presenting with net myositic morphological features- Insights from an autopsy study
Neuromuscular Disorders. 03 Feb [Epub ahead of print]. -
Schroeter, C.B. et al. (2024) Inter-alpha-trypsin inhibitor heavy chain H3 is a potential biomarker for disease activity in myasthenia gravis.
Acta Neuropathol. 147 (1): 102. -
Kleefeld, F. et al. (2022) Morphologic and Molecular Patterns of Polymyositis With Mitochondrial Pathology and Inclusion Body Myositis.
Neurology. 99 (20): e2212-e2222.
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