alamarBlue® scientific resources


The fast, non-toxic, simple and reliable cell proliferation and viability reagent

alamarBlue® - Cell Proliferation and Viability Reagent

alamarBlue® - Cell Proliferation and Viability Reagent

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alamarBlue® is a cell proliferation assay that provides a rapid, sensitive and economical way to quantitatively measure cell proliferation and cytotoxicity in various human and animal cell lines. It can also be used to study mycobacteria, bacteria and fungi.

The following features and benefits establish alamarBlue® as a preferred alternative reagent to similar cell proliferation assays (MTT, XTT, 3H Thymidine or neutral red) which will help you to get the results you need:

  • Simple  Water soluble, just add and measure the formula in cell cultures 
  • Flexible:  Colorimetric and fluorometric detection, for both suspended and attached cell lines
  • Non-toxic:  alamarBlue® does not kill cells and is safe to the user and the environment
  • Reliable:  Highly referenced for cytotoxicity and viability assays
  • Scalable:  Effortless scale up for high throughput assays
  • High Sensitivity:  Detects as few as 50 cells
  • Stable:  Proprietary buffering agents ensures alamarBlue® is suitable for time course studies
  • Economical:  No cell lysis allows cells to be cultured for subsequent assays


alamarBlue® Ordering Information

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code

    Key to Applications: IF immunofluorescence, E ELISA and FN functional assays * Please refer to datasheet for further information.

    alamarBlue® is offered in two sizes:

    • 25 ml = Enough for 2500 wells /96-well plate
    • 100 ml = Enough for 10000 wells /96-well plate

    Note: Calculations assume 100 µl final volume per well (96-well plate).

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    alamarBlue® is manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959.