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IgG antibody

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Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 1 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 2 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 3 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 4 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 5 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 6 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 7 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 8 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 9
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 1
HeLa cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using a Mouse monoclonal antibody specific for the human tubulin alpha 4A chain followed by AlexaFluor 488 conjugated Goat anti Mouse IgG antibodySTAR117D488GA, Goat anti Mouse IgG DyLight®488 at 1/50. Image was visualized on the Bio-Rad ZOE™ Fluorescent Cell Imager at 60x magnification
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 2
HeLa cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 μg/ml DAPI. Immunofluorescence was carried out using a Mouse monoclonal antibody specific for human tubulin beta 2Cfollowed by STAR117D488GA, Goat anti Mouse IgG DyLight®488 1/50. Image was visualized on the Bio-Rad ZOE™ Fluorescent Cell Imager at 60x magnification.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 3
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 4
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®649 (STAR117D649GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 5
Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 μg (lanes 2 & 7), 5 μg (lanes 3 & 8), 1 μg (lanes 4 & 9) Sheep anti NFkB p65 antibody ( AHP394), and 10 μg polyclonal IgG (lanes 5 & 10).

8.75 μl of each IP was loaded onto an AnykD ™ Criterion ™ TGX Stain-Free™ gel. Precision Plus Protein™ Prestained Standards were run lanes 1 & 6.

Mouse anti NFkB p65 (MCA6021) was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc™ Touch Imaging System. Arrow points to NFkB (molecular weight 65 kDa).
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 6
Paraffin section of human colon adenocarcinoma (HIER citrate pH6 antigen retrieval) blocked with 10% FCS and stained with mouse anti human cytokeratin 18 (MCA1864H) and detected with goat anti mouse IgG:A488 (STAR117D488GA). The tissue was also counterstained with PureBlu Hoechst 33342 (135-1304).
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 7
HeLa cells were treated with BrdU for 3 hours and stained with mouse anti-BrdU antibody, clone Bu20a (MCA2483). Goat anti mouse IgG (H/L) DyLight® 549 conjugated antibody (red) (STAR117D549GA) was used as the secondary antibody. Cytoplasm was stained with rabbit anti-GAPDH antibody (AHP1628). As the secondary antibody, sheep anti rabbit IgG DyLight® 488 conjugated antibody (green) (STAR36D488GA) was used. PureBlu™ DAPI (1351303) was used as nuclear counterstain (blue).
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 8
Figure A. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Isotype control detected with Goat anti Mouse IgG:FITC (STAR117F). Figure B. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Mouse anti Dog CD3 (MCA1774GA) detected with Goat anti Mouse IgG:FITC (STAR117F). All experiments performed on red cell lysed dog blood gated on lymphocytes in the presence of 10% dog serum. Data acquired on the ZE5™ Cell Analyzer.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 9
Figure A. RPE conjugated Rat anti Dog CD8 (MCA1039PE) and Isotype control detected with Goat anti Mouse IgG: FITC (STAR117F). Figure B. RPE conjugated Rat anti Dog CD8 (MCA1039PE) and Mouse anti Dog CD11d (MCA1779GA) detected with Goat anti Mouse IgG:FITC (STAR117F). All experiments performed on red cell lysed dog blood gated on lymphocytes in the presence of 10% dog serum. Data acquired on the ZE5™ Cell Analyzer.

Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L) (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):DyLight®405 (Multi Species Adsorbed)

Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR117A E WB 0.5 mg
STAR117F F 0.5 mg
STAR117P E WB 0.5 mg
STAR117 E F WB 0.5 mg
STAR117D549GA F IF WB 0.1 mg
STAR117D649GA F IF 0.1 mg
STAR117D488GA F IF 0.1 mg
STAR117D800GA F IF WB 0.1 mg
STAR117D405GA F IF 0.1 mg
STAR117D680GA F WB 0.1 mg
Goat anti Mouse IgG antibody recognizes mouse IgG and light chains common to other mouse immunoglobulin classes.

Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.

Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFα and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry (Redondo et al. 2013).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to DyLight®549 - liquid
Product Form
Purified IgG conjugated to DyLight®649 - liquid
Product Form
Purified IgG conjugated to DyLight®488 - liquid
Product Form
Purified IgG conjugated to DyLight®800 - liquid
Product Form
Purified IgG conjugated to DyLight®405 - liquid
Product Form
Purified IgG conjugated to DyLight®680 - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen.
Buffer Solution
50 mM HEPES pH 7.1
0.1M NaCl
1mM MgCl2
0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.05% Proclin™ 300
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Whole mouse IgG
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch

More Information

UniProt
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GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/50000
Western Blotting 1/2500 1/25000
Flow Cytometry 1/50 1/200
ELISA 1/10,000 1/100,000
Western Blotting 1/1000 1/10,000
ELISA 1ug/ml 10ug/ml
Flow Cytometry
Western Blotting
Flow Cytometry 1/50 1/400
Immunofluorescence 1/50 1/400
Western Blotting 1/500 1/1000
Flow Cytometry 1/500 1/1000
Immunofluorescence 1/500 1/1000
Flow Cytometry 1/400 1/800
Immunofluorescence
Flow Cytometry
Immunofluorescence
Western Blotting 1/10000 1/50000
Flow Cytometry 1/25 1/250
Immunofluorescence
Flow Cytometry 1/500 1/1000
Western Blotting 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 50 ul of the suggested working dilution to label 1x106 cells in 100ul
Western Blotting
Bio-Rad recommends STAR88P as the detection reagent for STAR117 in western blotting applications.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG antibody

  1. Abdala-Valencia, H. et al. (2012) Vitamin E isoforms differentially regulate intercellular adhesion molecule-1 activation of PKCα in human microvascular endothelial cells.
    PLoS One. 7: e41054.
  2. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  3. Banerjee, K. et al. (2012) Occluding the mannose moieties on human immunodeficiency virus type 1 gp120 with griffithsin improves the antibody responses to both proteins in mice.
    AIDS Res Hum Retroviruses. 28 (2): 206-14.
  4. Singh, S.M. et al. (2016) Characterization of Immune Responses to an Inactivated Avian Influenza Virus Vaccine Adjuvanted with Nanoparticles Containing CpG ODN.
    Viral Immunol. Apr 14. [Epub ahead of print]
  5. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
    Vet Immunol Immunopathol. 164 (1-2): 87-92.
  6. Askari, N. et al. (2015) Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation.
    Neuroscience. 305: 197-208.
  7. Topoluk, N. et al. (2017) Amniotic Mesenchymal Stromal Cells Exhibit Preferential Osteogenic and Chondrogenic Differentiation and Enhanced Matrix Production Compared With Adipose Mesenchymal Stromal Cells.
    Am J Sports Med. : 363546517706138.
  8. Alimolaei, M. et al. (2017) A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens α-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis.
    Probiotics Antimicrob Proteins. Apr 11 [Epub ahead of print].
  9. Schmidli, M.R. et al. (2018) Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease.
    BMC Vet Res. 14 (1): 161.
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