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IgG antibody

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Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 1 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 2 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 3 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 4 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 5 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 6 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 7 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 8 Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody thumbnail image 9
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 1
HeLa cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 µg/ml DAPI. Immunofluorescence was carried out using a Mouse monoclonal antibody specific for the human tubulin alpha 4A chain followed by AlexaFluor 488 conjugated Goat anti Mouse IgG antibodySTAR117D488GA, Goat anti Mouse IgG DyLight®488 at 1/50. Image was visualized on the Bio-Rad ZOE Fluorescent Cell Imager at 60x magnification
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 2
HeLa cells were fixed with 3% PFA and permeabilized with 0.3% Triton X-100. Nuclei were stained with 1.5 μg/ml DAPI. Immunofluorescence was carried out using a Mouse monoclonal antibody specific for human tubulin beta 2Cfollowed by STAR117D488GA, Goat anti Mouse IgG DyLight®488 1/50. Image was visualized on the Bio-Rad ZOE Fluorescent Cell Imager at 60x magnification.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 3
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight 488 shown in green.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 4
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®649 (STAR117D649GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight 488 shown in green.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 5
Western blot analysis of NFkB IP samples. IP was performed on Jurkat cell lysates using 10 μg (lanes 2 & 7), 5 μg (lanes 3 & 8), 1 μg (lanes 4 & 9) Sheep anti NFkB p65 antibody, and 10 μg polyclonal IgG (lanes 5 & 10).

8.75 μl of each IP was loaded onto an AnykD Criterion TGX Stain-Free gel. Precision Plus Protein Prestained Standards were run lanes 1 & 6.

Mouse anti NFkB p65 antibody was used at 1/1000 in lanes 1-5. HRP conjugated Goat anti Mouse IgG (H/L) antibody (STAR117P) was used at 1/10,000 and visualized on the Bio-Rad Chemidoc Touch Imaging System. Arrow points to NFkB (molecular weight 65 kDa).
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 6
Paraffin section of human colon adenocarcinoma (HIER citrate pH6 antigen retrieval) blocked with 10% FCS and stained with mouse anti human cytokeratin 18 antibody and detected with goat anti mouse IgG:A488 (STAR117D488GA). The tissue was also counterstained with PureBlu Hoechst 33342.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 7
HeLa cells were treated with BrdU for 3 hours and stained with mouse anti-BrdU antibody, clone Bu20a (MCA2483). Goat anti mouse IgG (H/L) DyLight 549 conjugated antibody (red) (STAR117D549GA) was used as the secondary antibody. Cytoplasm was stained with rabbit anti-GAPDH antibody (AHP1628). As the secondary antibody, sheep anti rabbit IgG DyLight 488 conjugated antibody (green) was used. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 8
Figure A. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Isotype control detected with Goat anti Mouse IgG:FITC (STAR117F). Figure B. RPE conjugated Rat anti Dog CD4 (MCA1038PE) and Mouse anti Dog CD3 (MCA1774GA) detected with Goat anti Mouse IgG:FITC (STAR117F). All experiments performed on red cell lysed dog blood gated on lymphocytes in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.
Anti Mouse IgG (H/L) (Multi Species Adsorbed) Antibody gallery image 9
Figure A. RPE conjugated Rat anti Dog CD8 (MCA1039PE) and Isotype control detected with Goat anti Mouse IgG: FITC (STAR117F). Figure B. RPE conjugated Rat anti Dog CD8 (MCA1039PE) and Mouse anti Dog CD11d (MCA1779GA) detected with Goat anti Mouse IgG:FITC (STAR117F). All experiments performed on red cell lysed dog blood gated on lymphocytes in the presence of 10% dog serum. Data acquired on the ZE5 Cell Analyzer.

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Goat anti Mouse IgG (H/L) (Multi Species Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

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STAR117
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Goat anti Mouse IgG antibody recognizes mouse IgG and light chains common to other mouse immunoglobulin classes.

Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.

Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFα and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry (Redondo et al. 2013).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography.
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisations of goats with highly purified antigen.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Whole mouse IgG
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 10ug/ml
Flow Cytometry
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 50 μl of the suggested working dilution to label 1x106 cells in 100μl

References for IgG antibody

  1. Abdala-Valencia, H. et al. (2012) Vitamin E isoforms differentially regulate intercellular adhesion molecule-1 activation of PKCα in human microvascular endothelial cells.
    PLoS One. 7: e41054.
  2. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  3. Banerjee, K. et al. (2012) Occluding the mannose moieties on human immunodeficiency virus type 1 gp120 with griffithsin improves the antibody responses to both proteins in mice.
    AIDS Res Hum Retroviruses. 28 (2): 206-14.
  4. Singh, S.M. et al. (2016) Characterization of Immune Responses to an Inactivated Avian Influenza Virus Vaccine Adjuvanted with Nanoparticles Containing CpG ODN.
    Viral Immunol. Apr 14. [Epub ahead of print]
  5. Iwaszko-Simonik, A. et al. (2015) Expression of surface platelet receptors (CD62P and CD41/61) in horses with recurrent airway obstruction (RAO).
    Vet Immunol Immunopathol. 164 (1-2): 87-92.
  6. Askari, N. et al. (2015) Tetracycline-regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse sciatic nerve regeneration upon transplantation.
    Neuroscience. 305: 197-208.
  7. Topoluk, N. et al. (2017) Amniotic Mesenchymal Stromal Cells Exhibit Preferential Osteogenic and Chondrogenic Differentiation and Enhanced Matrix Production Compared With Adipose Mesenchymal Stromal Cells.
    Am J Sports Med. : 363546517706138.
  8. Alimolaei, M. et al. (2017) A Recombinant Probiotic, Lactobacillus casei, Expressing the Clostridium perfringens α-toxoid, as an Orally Vaccine Candidate Against Gas Gangrene and Necrotic Enteritis.
    Probiotics Antimicrob Proteins. Apr 11 [Epub ahead of print].
    9. View The Latest Product References
  9. Schmidli, M.R. et al. (2018) Inflammatory pattern of the infrapatellar fat pad in dogs with canine cruciate ligament disease.
    BMC Vet Res. 14 (1): 161.
  10. Li, T. et al. (2021) RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.
    Nat Commun. 12 (1): 1055.
  11. Dicks, M.D.J. et al. (2022) Modular capsid decoration boosts adenovirus vaccine-induced humoral and cellular immunity against SARS-CoV-2
    bioRxiv: Feb 22. [Epub ahead of print].

RRID
AB_324488
UniProt
P01837
P01869
P01867
P01864
P01843
P01865
P01844
P01868
P01724
P03987
P01863
P01845
Entrez Gene
Iglv1
Igk-C
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

STAR117

150800 155733 157127

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