CD34 antibody | QBEND/10

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Mouse anti Human CD34:Biotin

Mouse anti Human CD34:FITC

Mouse anti Human CD34

Mouse anti Human CD34:RPE

Product Type
Monoclonal Antibody
Clone
QBEND/10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA547B F 100 Tests
MCA547F F 100 Tests/1ml
MCA547GT EM F IF IP P WB 25 µg
MCA547G C EM F IF IP P WB 0.2 mg
MCA547PET F 25 Tests/0.25ml
MCA547PE F 100 Tests
Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufman et al. 2001) and small vessel endothelium (Ramani et al. 1990)

Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Mouse anti Human CD34 antibody, clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Mouse anti Human CD34 antibody, clone QBEND/10 binds to a different eoptope to Mouse anti Human CD34, clone 581 (MCA1578) which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte Typing VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typing VI).

Mouse anti Human CD34 antibody, clone QBEND/10 has been successfully exploited for the detection of CD34 in brain capillaries of Alzheimer's patients (Kalaria et al. 1992) and in acute lymphoblastic leukemia cells (Sutherland et al. 1992) by western blotting.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Sheep
Rat
Dog
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Pack Size: 25 Tests/0.25ml
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Pack Size: 100 Tests
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests/0.25ml
Reconstitute with 0.25 ml distilled water
Pack Size: 100 Tests
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
Pack Size: 25 Tests/0.25ml
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Pack Size: 100 Tests
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Human endothelial cell membrane vesicles.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized NZB mice were fused with cells of the mouse NSO myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Pack Size: 25 Tests/0.25ml
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
18 months from the date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P28906 Related reagents
Entrez Gene
CD34 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0030246 carbohydrate binding
GO:0009897 external side of plasma membrane
GO:0016337 cell-cell adhesion
GO:0050776 regulation of immune response
GO:0050900 leukocyte migration
Regulatory
For research purposes only

Applications of CD34 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/10
Flow Cytometry 1/100 1/200
Immuno-electron Microscopy
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin 1/500 1/1000
Immunoprecipitation
Western Blotting
Flow Cytometry Neat Neat Pack Size: 25 Tests/0.25ml
1/10 Pack Size: 100 Tests
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Histology Positive Control Tissue
Tonsil, Bone Marrow.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Flow Cytometry

Immunofluorescence

Product Specific References

References for CD34 antibody

  1. Fina, L. et al. (1990) Expression of the CD34 gene in vascular endothelial cells.
    Blood. 75 (12): 2417-26.
  2. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  3. Chan-Ling T (2011) Role of CD44+ Stem Cells in Mural Cell Formation in the Human Choroid: Evidence of Vascular Instability Due to Limited Pericyte Ensheathment.
    Invest Ophthalmol Vis Sci. 52: 399-410.
  4. Pammer, J. et al. (1996) CD40 antigen is expressed by endothelial cells and tumor cells in Kaposi's sarcoma.
    Am J Pathol. 148 (5): 1387-96.
  5. Lee, M.Y. et al. (2009) Angiogenesis in differentiated placental multipotent mesenchymal stromal cells is dependent on integrin alpha5beta1.
    PLoS One. 4: e6913.
  6. Chan-Ling, T. et al. (2004) Astrocyte-endothelial cell relationships during human retinal vascular development.
    Invest Ophthalmol Vis Sci. 45: 2020-32.
  7. Chen, S.P. et al. (2014) Reduced circulating endothelial progenitor cells in reversible cerebral vasoconstriction syndrome.
    J Headache Pain. 15: 82.
  8. Sauer, G. et al. (2003) Progression of cervical carcinomas is associated with down-regulation of CD9 but strong local re-expression at sites of transendothelial invasion.
    Clin Cancer Res. 9: 6426-31.
  9. Sauter, B. et al. (1998) Immunoelectron Microscopic Characterization of Human Dermal Lymphatic Microvascular Endothelial Cells: Differential Expression of CD31, CD34, and Type IV Collagen with Lymphatic Endothelial Cells vs Blood Capillary Endothelial Cells in Normal Human Skin, Lymphangioma, and Hemangioma In Situ.
    J Histochem Cytochem. 46: 165-76.
  10. Shetty, S. et al. (2011) Common lymphatic endothelial and vascular endothelial receptor-1 mediates the transmigration of regulatory T cells across human hepatic sinusoidal endothelium.
    J Immunol. 186: 4147-55.
  11. Zhao, M. et al. (2007) Evidence for the presence of stem cell-like progenitor cells in human adult pancreas.
    J Endocrinol. 195: 407-14.
  12. Jokubaitis, V.J. et al. (2008) Angiotensin-converting enzyme (CD143) marks hematopoietic stem cells in human embryonic, fetal, and adult hematopoietic tissues.
    Blood. 111: 4055-63.
  13. Rutella, S. et al. (2003) Identification of a novel subpopulation of human cord blood CD34-CD133-CD7-CD45+lineage- cells capable of lymphoid/NK cell differentiation after in vitro exposure to IL-15.
    J Immunol. 171: 2977-88.
  14. Suzuki, M. et al. (2012) Induction of human humoral immune responses in a novel HLA-DR-expressing transgenic NOD/Shi-scid/γcnull mouse.
    Int Immunol. 24 (4): 243-52.
  15. Hsieh, J.Y. et al. (2013) miR-146a-5p circuitry uncouples cell proliferation and migration, but not differentiation, in human mesenchymal stem cells.
    Nucleic Acids Res. 41 (21): 9753-63.
  16. Blank A et al. (2010) SDHB loss predicts malignancy in pheochromocytomas/sympathethic paragangliomas, but not through hypoxia signalling.
    Endocr Relat Cancer. 17 (4): 919-28.
  17. Junaid TO et al. (2014) Fetoplacental vascular alterations associated with fetal growth restriction.
    Placenta. 35 (10): 808-15.
  18. Beleut M et al. (2012) Integrative genome-wide expression profiling identifies three distinct molecular subgroups of renal cell carcinoma with different patient outcome.
    BMC Cancer. 12: 310.
  19. Chan-Ling T et al. (2011) Evidence of hematopoietic differentiation, vasculogenesis and angiogenesis in the formation of human choroidal blood vessels.
    Exp Eye Res. 92 (5): 361-76.
  20. Motamedian, S.R. et al. (2016) Response of Dental Pulp Stem Cells to Synthetic, Allograft, and Xenograft Bone Scaffolds.
    Int J Periodontics Restorative Dent. 37 (1): 49-59.
  21. Fan, C-Y. et al. (2017) De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Biochemistry and Biophysics Reports. 9: 51-60.
  22. Sameshima, N. et al. (2011) So-called 'adenosarcoma' of the kidney a novel adult renal tumor with a cystic appearance.
    Pathol Int. 61 (5): 313-8.
  23. Grognuz, A. et al. (2016) Human Fetal Progenitor Tenocytes for Regenerative Medicine.
    Cell Transplant. 25 (3): 463-79.
  24. Wang, D.Y. et al. (2017) Histological component quantification for the evaluation of endometrial receptivity in women with natural cycles undergoing in vitro fertilization/intracytoplasmic sperm injection.
    Taiwan J Obstet Gynecol. 56 (3): 368-370.
  25. GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
    PLoS One. 13 (2): e0192244.

Further Reading

  1. Gorr, T.A. et al. (2011) Old proteins - new locations: myoglobin, haemoglobin, neuroglobin and cytoglobin in solid tumours and cancer cells.
    Acta Physiol (Oxf). 202: 563-581.