BrdU Antibodies and Applications

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  • RF06
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  • AbD33758kd
  • AbD33761kd
  • Bu20a
  • Rabbit Anti-BrdU
  • BrdU range

Anti-BrdU antibodies recognize BrdU that has been incorporated into DNA, instead of thymidine, during the S-phase of a cell cycle. To support your proliferation studies, Bio-Rad has expanded its range of highly validated PrecisionAb Anti-BrdU Antibodies.

BrDu Staining Experiments 10 Tips for Success Quick Guide

BrDu Staining Experiments 10 Tips for Success Quick Guide

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These antibodies are tested in multiple applications and for cross-reactivity with thymidine and other thymidine analogs. Detailed protocols for various immunodetection applications provide guidance to optimize their use. Check out the wide range of PrecisionAb Anti-BrdU Antibodies below (and the appropriate application use) and find the right one for your experimental needs.

Fig.1. Jurkat cells were treated with 100 μM BrdU and stained with Rat Anti-BrdU Antibody clone RF04-2 (MCA6143).

Fig. 1. Jurkat cells were treated with 100 μM BrdU and stained with Rat Anti-BrdU Antibody clone RF04-2 (MCA6143). As a secondary antibody, Rabbit F(ab')2 Anti-Rat IgG: DyLight488 conjugated Antibody (STAR16D488GA) was used at a 1/400 dilution. ReadiDrop Propidium Iodide was used to stain total DNA.

PrecisionAb Rat Anti-BrdU Antibody, clone RF04-2
​

The Rat Anti-BrdU Antibody, clone RF04-2 (MCA6143) has been tested in flow cytometry. The antibody cross-reacts with CIdU, EdU, and IdU in flow cytometry. Importantly, it does not cross-react with thymidine, so you don’t have to worry about undesired background signal affecting your results. We provide a detailed protocol including BrdU labeling and DNA denaturation step. This denaturation step allows anti-BrdU antibodies to access the incorporated BrdU in the single-stranded DNA. The isotype of the antibody is IgG2b.
 



Fig. 2. HeLa cells were treated with BrdU and stained with Rat Anti-BrdU Antibody clone RF06 (MCA6144).

Fig. 2. HeLa cells were treated with BrdU and stained with Rat Anti-BrdU Antibody clone RF06 (MCA6144). Goat Anti-Rat IgG (H/L):TRITC Conjugated Antibody (red) (305003) was used as a secondary antibody. PureBlu DAPI (1351303) was used as nuclear counterstain. Co-staining with Rabbit Anti-GADPH, with Sheep Anti-Rabbit IgG:DyLight488 Conjugated Antibody (green) (STAR36D488GA) as the secondary detection antibody, was also performed to visualize the cytoplasm and confirm specificity of BrdU staining to the nucleus.

PrecisionAb Rat Anti-BrdU Antibody, clone RF06​

 

The Rat Anti-BrdU Antibody, clone RF06 (MCA6144) has been tested in immunocytochemistry. Immunocytochemical BrdU labeling is a quick and highly reproducible method, widely used to study the cytokinetics of normal and neoplastic cells. An easy-to-follow, step-by-step protocol outlining how to perform immunofluorescent BrdU staining is available. The isotype of the antibody is IgG2a.
 



Fig. 3. HeLa cells were treated with 100 μM BrdU and stained with Human Anti-BrdU Antibody (HCA320) at a 1/500 dilution

Fig. 3. HeLa cells were treated with 100 μM BrdU and stained with Human Anti-BrdU Antibody (HCA320) at a 1/500 dilution. Sheep Anti-Rabbit: DyLight549 Conjugated Antibody (STAR36D549GA) at a 1/500 dilution was used as a secondary antibody. PureBlu DAPI (1351303) was used as nuclear counterstain.

PrecisionAb Human Anti-BrdU Antibody, clone AbD33758kg
​

The Human Anti-BrdU Antibody, clone AbD33758kg (HCA320) is a recombinant, chimeric human/rabbit IgG antibody selected from the HuCAL® phage display library. In this antibody, the VH-CH1 regions and light chains are human while the Fc region is derived from rabbit IgG. The antibody has been tested in flow cytometry and immunocytochemistry. It cross-reacts with CIdU in flow cytometry. Therefore, CIdU can be used in the studies instead of BrdU. It does not cross-react with thymidine. A detailed protocol for using this clone AbD33758kg is available on the BrdU staining protocols webpage.



Fig. 4. Jurkat cells were treated with 100 μM BrdU.

Fig. 4. Jurkat cells were treated with 100 μM BrdU. Cells were stained with Human Anti-BrdU Antibody (HCA321) at a 1/2,000 dilution for 1 hr at RT. As a secondary antibody, Sheep Anti-Rabbit:DyLight488 Conjugated Antibody (STAR36D488GA) was used at a 1/200 dilution. ReadiDrop Propidium Iodide was used to stain total DNA.

PrecisionAb Human Anti-BrdU Antibody, clone AbD33761kd
​

The Human Anti-BrdU Antibody, clone AbD33761kd (HCA321) is a recombinant, chimeric human/rabbit IgG antibody selected from the HuCAL phage display library. In this antibody, the VH-CH1 regions and light chains are human, while the Fc region is derived from rabbit IgG. The antibody has been tested in flow cytometry and cross-reacts with CIdU. Therefore, CIdU can be used in the studies instead of BrdU. It does not cross-react with thymidine. A detailed protocol for using clone AbD33761kd in flow cytometry is available on the BrdU staining protocols webpage.
 



Fig. 5. Jurkat cells were treated with 100 μM BrdU.

Fig. 5. Jurkat cells were treated with 100 μM BrdU. Cells were stained with Human Anti-BrdU Antibody (HCA322) at a 1/2,000 dilution for 1 hr at RT. ReadiDrop Propidium Iodide was used to stain total DNA.

 

PrecisionAb Human Anti-BrdU Antibody, clone AbD33758kd
​

The Human Anti-BrdU Antibody, clone AbD33758kd (HCA322) is a recombinant, chimeric human/rat IgG2a antibody selected from the HuCAL phage display library. In this antibody, the VH and VL regions are human while the CH1-CH3 and C-lambda regions are derived from rat IgG2a. It is recommended to use in flow cytometry and immunocytochemistry. In flow, it cross-reacts with CIdU and IdU. Therefore, CIdU and IdU can be used in the studies instead of BrdU. It does not cross-react with thymidine. A detailed protocol for using clone AbD33758kd in flow cytometry can be found on the BrdU staining protocols webpage.



Fig. 6. Jurkat cells were treated with 100 μM BrdU

Fig. 6. Jurkat cells were treated with 100 μM BrdU. Cells were stained with Human Anti-BrdU Antibody (HCA323) at a 1/2,000 dilution for 1 hr at RT. Sheep Anti-Rat: DyLight488 Conjugated Antibody (STAR16D488GA) was used as a secondary antibody at a 1/200 dilution. Propidium Iodide was used to stain total DNA.

PrecisionAb Human Anti-BrdU Antibody, clone AbD33761kd
​

The Human Anti-BrdU Antibody, clone AbD33761kd (HCA323) is a recombinant, chimeric human/rat IgG2a antibody selected from the HuCAL phage display library. In this antibody, the VH and VL regions are human, while the CH1-CH3 and C-lambda regions are derived from rat IgG2a. The antibody is recommended for use in flow cytometry and immunocytochemistry. In flow cytometry, it cross-reacts with CIdU. Therefore, CIdU can be used in the studies instead of BrdU. It does not cross-react with thymidine. A detailed protocol for using clone AbD33761kd is available on the BrdU staining protocols webpage.
 



Fig. 7. HeLa cells were treated with BrdU for 3 hours and stained with Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483, red)

Fig. 7. HeLa cells were treated with BrdU for 3 hours and stained with Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483, red). Cytoplasm was stained with Rabbit Anti-GAPDH antibody (AHP1628, green). PureBlu DAPI (1351303) was used as nuclear counterstain (blue).

Mouse Anti-BrdU Antibody, clone Bu20a
​

The Mouse Anti-BrdU Antibody, clone Bu20a, (MCA2483) has been tested in flow cytometry and immunocytochemistry. This antibody has been referenced in 14 publications. It is available in three different sizes (0.2 mg, 0.1 mg, and 20 µg) and conjugated to FITC (MCA2483FA). In addition to flow cytometry and immunocytochemistry, the Bu20a clone is recommended for immunohistochemistry.






 

Rabbit Anti-BrdU Antibody
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The Rabbit Anti-BrdU Antibody (AHP2405) has been tested in flow cytometry and immunocytochemistry and we provide detailed protocols for using the antibody in these applications. In addition to these two applications, the antibody is also supported by immunohistochemistry data.

Fig. 8. BrdU-labeled human lymphoma cells were stained with Rabbit Anti-BrdU Antibody (AHP2405) at a 1/50 dilution (A) and a 1/100 dilution (B).
Fig. 8. BrdU-labeled human lymphoma cells were stained with Rabbit Anti-BrdU Antibody (AHP2405) at a 1/50 dilution (A) and a 1/100 dilution (B). Sheep Anti Rabbit IgG DyLight Conjugated Secondary Antibody (STAR36D488GA) was used as the secondary detection reagent at a 1/50 dilution. ReadiDrop Propidium Iodide (1351101) was used to stain total DNA.



BrdU Antibodies

Bio-Rad's range of BrdU antibodies comes in a number of formats and can be used for applications including flow cytometry, immunofluorescence and immunohistochemistry.

 

Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance please do not hesitate to contact us.

Our BrdU Antibody Range

    DescriptionSpecificityTargetFormatHostIsotypeClone Applications Citations Product Type Code
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