BrdU antibody | RF06
Rat anti BrdU antibody, clone RF06 (MCA6144) used to label BrdU incorporating celly in rat dentate gyrus by immunofluorescence.
Image caption:
Counting BrdU-positive cells in the granule cell layer and about two cell diameters of the SGZ. The apex of the adolescent rat dentate gyrus showing NeuN (green) and BrdU (red) immunoreactivity is imaged. (A) The dentate gyrus and subgranular zone outlined in the overlayed image. (B) NeuN immunoreactivity. (C) BrdU immunoreactivity. (D) A magnified portion of the granule cell layer showing BrdU immunoreactivity used for cell counting.
From: Hovorka M, Ewing D, Middlemas DS.
Chronic SSRI Treatment, but Not Norepinephrine Reuptake Inhibitor Treatment, Increases Neurogenesis in Juvenile Rats.
Int J Mol Sci. 2022 Jun 22;23(13):6919.
doi: 10.3390/ijms23136919.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti BrdU
- Product Type
- Monoclonal Antibody
- Clone
- RF06
- Isotype
- IgG2a
- Format
- Purified
- Specificity
- BrdU
Antibody Quality and Performance Guarantee
| Rat anti BrdU antibody, clone RF06 recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated into the newly synthesized DNA during replication instead of thymidine. Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004). BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Rat anti BrdU antibody, clone RF06, has been tested in immunofluorescence. |
|
- Target Species
- Chemical
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 5% Trehalose
- Immunogen
- Bromodeoxyuridine
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence |  |
1/500 | |
| Immunohistology - Paraffin | |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for BrdU antibody
-
Wang, M. et al. (2023) Eucommiae cortex polysaccharides attenuate gut microbiota dysbiosis and neuroinflammation in mice exposed to chronic unpredictable mild stress: Beneficial in ameliorating depressive-like behaviors.
J Affect Disord. 334: 278-292. -
Hovorka, M. et al. (2022) Chronic SSRI Treatment, but Not Norepinephrine Reuptake Inhibitor Treatment, Increases Neurogenesis in Juvenile Rats.
Int J Mol Sci. 23 (13): 6919. -
Zhang, J. et al. (2024) Comparison of genes involved in brain development: insights into the organization and evolution of the telencephalic pallium
Scientific Reports. 14 (1) : 6102 -
Ruscu, M. et al. (2024) The post-stroke young adult brain has limited capacity to re-express the gene expression patterns seen during early postnatal brain development.
Brain Pathol. 34 (5): e13232.
- Synonyms
- 5-BROMODEOXYURIDINE
MCA6144
100004195
100005776
100006654
100007800
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