BrdU antibody | RF06

Rat anti BrdU

Product Type
Monoclonal Antibody
Clone
RF06
Isotype
IgG2a
Format
Purified
Specificity
BrdU

Product Code Applications Pack Size List Price Your Price Qty
MCA6144
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti BrdU antibody, clone RF06 recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated into the newly synthesized DNA during replication instead of thymidine.

Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants.

Rat anti BrdU antibody, clone RF06, has been tested in immunofluorescence.
PrecisionAb Antibodies

Target Species
Chemical
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
5% Trehalose
Immunogen
Bromodeoxyuridine
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/500
Immunohistology - Paraffin

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Synonyms
5-BROMODEOXYURIDINE
View more products with BRDU specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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