BrdU antibody

Rabbit anti BrdU

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BrdU

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AHP2405
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Rabbit anti BrdU antibody recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.

BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

Target Species
Chemical
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to BrdU was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Bromodeoxyuridine conjugated to KLH
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunocytochemistry 1/500
Immunofluorescence 2 1/100 1/500
Immunohistology - Paraffin 1/100 1/500
  1. 1Flow Cytometry protocols can be found at:
    BrdU Staining of Cells with Rabbit Anti-BrdU Antibody by Flow Cytometry.
  2. 2BrdU labeling and immunostaining protocol can be found at:
    www.bio-rad-antibodies.com/rabbit-brdu-labeling-and-immunostaining-protocol
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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