BrdU antibody
>Visit our BrdU resources page
> Visit our BrdU resources page
> Visit our BrdU resources page
Rabbit anti BrdU antibody recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.
BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
The cross reactivity of Rabbit anti BrdU antibody to other thymidine analogs, such as 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU) has not been analyzed. However, the antibody does not appear to detect endogenous thymidine.
BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
The cross reactivity of Rabbit anti BrdU antibody to other thymidine analogs, such as 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU) has not been analyzed. However, the antibody does not appear to detect endogenous thymidine.
Product Details
- Target Species
- Chemical
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to BrdU was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Bromodeoxyuridine conjugated to KLH
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of BrdU antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | 1/50 | 1/100 | |
| Immunocytochemistry | 1/500 | ||
| Immunofluorescence 2 | 1/100 | 1/500 | |
| Immunohistology - Paraffin | 1/100 | 1/500 |
- 1Flow Cytometry protocols can be found at:
BrdU Staining of Cells with Rabbit Anti-BrdU Antibody by Flow Cytometry. - 2BrdU labeling and immunostaining protocol can be found at:
www.bio-rad-antibodies.com/rabbit-brdu-labeling-and-immunostaining-protocol
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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