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BrdU antibody

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Anti BrdU Antibody thumbnail image 1 Anti BrdU Antibody thumbnail image 2 Anti BrdU Antibody thumbnail image 3 Anti BrdU Antibody thumbnail image 4 Anti BrdU Antibody thumbnail image 5 Anti BrdU Antibody thumbnail image 6
Anti BrdU Antibody gallery image 1
Immunofluorescence microscopy image of paraformaldehyde-fixed, paraffin-embedded guinea pig pancreas tissue stained with 2.7 μg/ml rabbit anti-BrdU antibody (AHP2405). Donkey anti-rabbit antibody was used as the secondary antibody (red). DAPI was used as nuclear counterstain (blue) and imaged with a 40× objective.

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Anti BrdU Antibody gallery image 2
HeLa cells were treated with 10 μg BrdU for 1 hour (B) or left untreated (A). Cells were stained with Rabbit anti-BrdU antibody (AHP2405) at a dilution of 1/500. As a secondary antibody, Sheep anti Rabbit IgG DyLight 549 conjugated antibody was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.

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Anti BrdU Antibody gallery image 3
BrdU-labeled human lymphoma cells were stained with rabbit anti-BrdU antibody (AHP2405) at a 1/50 dilution (A) and a 1/100 dilution (B). Sheep anti Rabbit IgG DyLight 488 conjugated antibody was used as the secondary detection reagent at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.

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Anti BrdU Antibody gallery image 4
Jurkat cells were treated with 10 μM BrdU (A), CldU (B), IdU (C), EdU (D), Thymidine (E) or left untreated (F). Cells were stained with 1 μg/ml Rabbit anti BrdU antibody (AHP2405) for 1 hour at room temperature. As a secondary antibody, Sheep anti Rabbit IgG DyLight 488 conjugated antibody was used at a 1/100 dilution. Propidium Iodide was used to stain total DNA.
Anti BrdU Antibody gallery image 5
HeLa cells treated with BrdU (A) or DMSO (B) and stained with Rabbit anti BrdU antibody (AHP2405) at a 1/100 dilution. Sheep anti Rabbit IgG DyLight 549 conjugated antibody (red) at a 1/500 dilution was used as the secondary antibody. Cytoplasm was stained with Mouse anti Human GAPDH antibody (VMA00046) at a 1/500 dilution. As the secondary antibody, Goat anti Mouse IgG (H/L) DyLight 488 conjugated antibody (green) (STAR117D488GA) was used at a 1/500 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain (blue).
Anti BrdU Antibody gallery image 6
Immunohistochemistry analysis showing staining of BrdU with Rabbit anti BrdU antibody (AHP2405) in the nucleus of paraffin-embedded Rat Jejunum treated with 100 mg/kg BrdU in PBS (A) compared to Rat Jejunum treated with PBS only (C). A negative control without primary antibody was also included for both BrdU treated (B) and untreated tissue (D). Detection was performed using an HRP conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

Rabbit anti BrdU

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
BrdU

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Product Code Applications Pack Size List Price Your Price Qty
AHP2405
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Rabbit anti BrdU antibody recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.

BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

Target Species
Chemical
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to BrdU was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Bromodeoxyuridine conjugated to KLH
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunocytochemistry 1/500
Immunofluorescence 2 1/100 1/500
Immunohistology - Paraffin 1/100 1/500
  1. 1Flow Cytometry protocols can be found at:
    BrdU Staining of Cells with Rabbit Anti-BrdU Antibody by Flow Cytometry.
  2. 2BrdU labeling and immunostaining protocol can be found at:
    www.bio-rad-antibodies.com/rabbit-brdu-labeling-and-immunostaining-protocol
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
READIDROP™ Propidium Iodide 1351101 F 3 X 3ml
List Price Your Price
Description READIDROP™ Propidium Iodide
PUREBLU™ DAPI 1351303 F IF 250 µg
List Price Your Price
Description PUREBLU™ DAPI

AHP2405

151396 154684 157294

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