Rabbit anti BrdU antibody
recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.
BrdU is also commonly used in vivo
for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004
) or in drinking water (Moser et al. 2004
- Target Species
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to BrdU was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Sodium Azide (NaN3)
- Bromodeoxyuridine conjugated to KLH
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of BrdU antibody
|Flow Cytometry 1
|Immunohistology - Paraffin
- 1Flow Cytometry protocols can be found at:
BrdU Staining of Cells with Rabbit Anti-BrdU Antibody by Flow Cytometry.
- 2BrdU labeling and immunostaining protocol can be found at:
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available