BrdU antibody

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Rabbit anti BrdU

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP2405 F* IC IF* P 50 µg
Rabbit anti BrdU antibody recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.

BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

The cross reactivity of Rabbit anti BrdU antibody to other thymidine analogs, such as 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU) has not been analyzed. However, the antibody does not appear to detect endogenous thymidine.

Product Details

Target Species
Chemical
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to BrdU was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Bromodeoxyuridine conjugated to KLH
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of BrdU antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/50 1/100
Immunocytochemistry 1/500
Immunofluorescence 2 1/100 1/500
Immunohistology - Paraffin 1/100 1/500
  1. 1Flow Cytometry protocols can be found at:
    BrdU Staining of Cells with Rabbit Anti-BrdU Antibody by Flow Cytometry.
  2. 2BrdU labeling and immunostaining protocol can be found at:
    www.bio-rad-antibodies.com/rabbit-brdu-labeling-and-immunostaining-protocol
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
READIDROP™ Propidium Iodide 1351101 3 X 3ml F
PUREBLU™ DAPI 1351303 250 µg F IF