READIDROP™ Propidium Iodide









Product Details
Storage Information
- Storage
- Store at room temperature
- Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of READIDROP™ Propidium Iodide
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/250 | 1/500 |
- Instructions For Use
- Caution: Wear gloves, safety glasses/face protection, and protective clothing to avoid direct contact of ReadiDrop solutions with skin and eyes. Refer to MSDS for more information.
1. Resuspend 1 x 106 cells in 500 μl flow cytometry buffer (1x phosphate buffered saline with 3% BSA). The cell concentration is now 2 x 106/ml.
2. Add one to two drop of ReadiDrop Propidium Iodide to the cell suspension.
3. Vortex briefly.
4. Incubate for 1-10 min at room temperature.
Note: Adding one drop to a 500 μl cell suspension produces a final dye concentration of 1 μg/ml. This is typically sufficient to stain 1 x 106 cells. If one drop is not sufficient for your cell type, adding a second drop may improve staining efficacy. When working with a larger volume of cells, scale ReadiDrop solution amount accordingly.
5. Analyze cells by flow cytometry.
- Detect PI signal in FL3 on Bio-Rad's S3e Cell Sorter. Otherwise, select the most appropriate FL channel on your particular instrument.
Product Specific References
References for READIDROP™ Propidium Iodide
-
Weldrick, P.J. et al. (2020) Super‐Enhanced Removal of Fungal Biofilms by Protease‐Functionalized Amphotericin B Nanocarriers
Adv NanoBiomed Res. 10 Dec [Epub ahead of print].
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra