BrdU antibody | RF04-2
Rat anti BrdU antibody, clone RF04-2 recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated into the newly synthesized DNA during replication instead of thymidine.
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants.
Rat anti BrdU antibody, clone RF04-2, has been tested in flow cytometry.
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants.
Rat anti BrdU antibody, clone RF04-2, has been tested in flow cytometry.
Product Details
- Target Species
- Chemical
- Product Form
- Purified IgG - lyophilized
- Reconstitution
- Reconstitute with 0.1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 5% Trehalose
- Immunogen
- Bromodeoxyuridine
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of BrdU antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/400 | 1/2000 | |
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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