BrdU antibody | RF04-2

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Rat anti BrdU

Product Type: Monoclonal Antibody
Clone: RF04-2
Isotype: IgG2b
Format: Purified
Specificity: BrdU

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Secondary Antibodies
References (2)
Applications
Storage Information
Batch/Lot Specific Datasheets

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MCA6143 Datasheet SDS SDS	F P	0.1 mg		Log in
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Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) thumbnail image 1

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) thumbnail image 2

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) thumbnail image 3

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) thumbnail image 4

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) thumbnail image 5

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) gallery image 1 — Jurkat cells were treated with 100 μM BrdU. Cells were stained with Rat anti BrdU antibody, clone RF04-2 (**MCA6143**) at a 1/400 dilution for 1 hour at room temperature. As a secondary antibody, DyLight^® 488 conjugated Rabbit F(ab′)₂ anti Rat IgG antibody (**STAR16D488GA**) was used at a 1/400 dilution. Propidium Iodide was used to stain total DNA.

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) gallery image 2 — Jurkat cells were treated with 100 μM BrdU. Cells were stained with Rat anti BrdU antibody, clone RF04-2 (**MCA6143**) at a 1/1600 dilution for 1 hour at room temperature. As a secondary antibody, Dylight^® 488 conjugated Rabbit F(ab′)₂ anti Rat IgG antibody (**STAR16D488GA**) was used at a 1/400 dilution. Propidium Iodide was used to stain total DNA.

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) gallery image 3 — Immunohistochemistry analysis showing staining of BrdU with Rat anti BrdU antibody, clone RF04-2 (**MCA6143**) in the nucleus of paraffin-embedded Rat Jejunum treated with 100 mg/kg BrdU in PBS (A) compared to Rat Jejunum treated with PBS only (C). A negative control without primary antibody was also included for both BrdU treated (B) and untreated tissue (D). Detection was performed using an HRP conjugated secondary antibody followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin.

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) gallery image 4 — Jurkat cells were treated with 100 μM BrdU (A), CldU (B), IdU (C), EdU (D) or DMSO (E). Cells were stained with Rat anti BrdU antibody, clone RF04-2 (**MCA6143**) at a 1/2000 dilution for 1 hour at room temperature. As a secondary antibody, Dylight^® 488 conjugated Rabbit F(ab′)₂ anti Rat IgG antibody (**STAR16D488GA**) was used. Propidium Iodide was used to stain total DNA.

Anti BrdU Antibody, clone RF04-2 (Monoclonal Antibody Antibody) gallery image 5 — **Published customer image:**
Rat anti BrdU antibody, clone RF04-2 (**MCA6143**) used to label proliferating cells in the hippocampus of mice by immunohistochemistry on formalin fixed sections.
**Image caption:**
Stimulation of Tph2-ChR2-YFP neurons in the dorsal and median raphe nuclei (1, DRN, MRN) to examine the effects on cell proliferation in the dentate gyrus (2, DG).
(B) Peroxidase staining to characterize BrdU-labeled cells in the subgranular zone (SGZ) of the hippocampus. Overnight light pulses to either the DRN or MRN significantly decreased the number of proliferating cells in the DG compared to the nonstimulated CTR groups. Scale bar represents 100 μm. BrdU, bromodeoxyuridine. Student’s t-tests, (*) p <0.05, (**) p <0.01.

From: Araragi N, Petermann M, Suzuki M, Larkum M, Mosienko V, Bader M, Alenina N, Klempin F.
Acute Optogenetic Stimulation of Serotonin Neurons Reduces Cell Proliferation in the Dentate Gyrus of Mice.
**ACS Chem Neurosci. 2025 16 (5): 781-789.** .
doi: 10.1021/acschemneuro.4c00771.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Rat anti BrdU antibody, clone RF04-2 recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated into the newly synthesized DNA during replication instead of thymidine.

Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants.

Rat anti BrdU antibody, clone RF04-2, has been tested in flow cytometry.

Target Species: Chemical
Product Form: Purified IgG - lyophilized
Reconstitution: Reconstitute with 0.1 ml distilled water
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 5% Trehalose
Immunogen: Bromodeoxyuridine
Approx. Protein Concentrations: IgG concentration 1.0 mg/ml
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		1/400	1/2000
Immunohistology - Paraffin

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Learn about how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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References for BrdU antibody

Araragi, N. et al. (2025) Acute Optogenetic Stimulation of Serotonin Neurons Reduces Cell Proliferation in the Dentate Gyrus of Mice.
ACS Chem Neurosci. Feb 12 [Epub ahead of print].
Horie, J. et al. (2025) Administration of paroxetine during pregnancy affects behavioral changes and hippocampal cell proliferation in male offspring in rats.
Pharmacol Biochem Behav. 253: 174041.