Tools to Accurately Access ProliferationDownload PDF ▸
BrdU (5′-bromo-2′-deoxyuridine) is a thymidine analog that has been established as a popular tool in cancer and neuroscience research for determining cell proliferation rates.
BrDu Staining Experiments 10 Tips for Success Quick Guide
Once added to samples, BrdU is incorporated into newly synthesized DNA during replication. BrdU can consequently be detected with anti-BrdU antibodies, such as Rat Anti-BrdU Antibody (clone RF04-2, MCA6143), Human Anti-BrdU Antibody (clone AbD33758kg, HCA320), Mouse Anti-BrdU Antibody (clone Bu20a, MCA2483), and Rabbit Anti-BrdU Antibody (AHP2405) thereby allowing the visualization of proliferating cells.
BrdU detection protocols:
Protocol describing how to BrdU label HeLa cells and detect the incorporated BrdU with the help of rabbit and mouse anti-BrdU antibodies.
Protocols highlighting how to detect incorporated BrdU by flow cytometry analysis.