Protocols: BrdU Labeling and Anti-BrdU Antibody Stainings

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Overview

BrdU (5′-bromo-2′-deoxyuridine) is a thymidine analog that has been established as a popular tool in cancer and neuroscience research for determining cell proliferation rates. 

Once added to samples, BrdU is incorporated into newly synthesized DNA during replication. BrdU can consequently be detected with anti-BrdU antibodies, such as Mouse Anti-BrdU Antibody, clone Bu20a (MCA2483) and Rabbit Anti-BrdU Antibody (AHP2405) thereby allowing the visualization of proliferating cells. Commonly used antibody based BrdU detection methods include flow cytometry, immunocytochemistry, and immunohistochemistry.

BrdU detection protocols: 


Product Specific Flow Cytometry Protocols

BrdU Labeling and Immunostaining Protocol

Protocol describing how to BrdU label HeLa cells and detect the incorporated BrdU with the help of rabbit and mouse anti-BrdU antibodies.

  1. BrdU Labeling of HeLa Cells Followed by Immunostaining with Mouse Anti-BrdU Antibody (clone Bu20a)
  2. BrdU Labeling of HeLa Cells Followed by Immunostaining with Rabbit Anti-BrdU Antibody
Product Specific Flow Cytometry Protocols

BrdU Detection by Flow Cytometry

Protocols highlighting how to detect incorporated BrdU by flow cytometry analysis.

  1. BrdU Staining of Cells for Cell Cycle Analysis
  2. BrdU Staining of Cells with Anti-BrdU Antibody (clone Bu20a)
  3. BrdU Staining of Cells with Rabbit
    Anti-BrdU Antibody