Tools to Accurately Assess ProliferationDownload PDF ▸
BrdU (5′-bromo-2′-deoxyuridine) is a thymidine analog that has been established as a popular tool in cancer and neuroscience research for determining cell proliferation rates.
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Once added to samples, BrdU is incorporated into newly synthesized DNA during replication. BrdU can consequently be detected with anti-BrdU antibodies, such as Rat Anti-BrdU Antibody (clone RF04-2, MCA6143), Human Anti-BrdU Antibody (clone AbD33758kg, HCA320), Mouse Anti-BrdU Antibody (clone Bu20a, MCA2483), and Rabbit Anti-BrdU Antibody (AHP2405) thereby allowing the visualization of proliferating cells.
BrdU detection protocols:
Protocol describing how to BrdU label HeLa cells and detect the incorporated BrdU with the help of rabbit and mouse anti-BrdU antibodies.
Protocols highlighting how to detect incorporated BrdU by flow cytometry analysis.