BrdU antibody | AbD33758kd
Human anti BrdU antibody, clone AbD33758kd recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analogue, which is incorporated into the newly synthesized DNA during replication instead of thymidine.
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analogue in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants.
Human anti BrdU antibody, clone AbD33758kd, has been tested in flow cytometry. Anti rat IgG2a specific antibodies are recommended as secondary detection reagents.
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analogue in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants.
Human anti BrdU antibody, clone AbD33758kd, has been tested in flow cytometry. Anti rat IgG2a specific antibodies are recommended as secondary detection reagents.
Product Details
- Target Species
- Chemical
- Product Form
- Recombinant chimeric human/rat IgG2a antibody selected from the HuCAL® phage display library and expressed in a human cell line. In this antibody, the VH and VL regions are human while the CH1-CH3 and C-lambda regions are derived from rat IgG2a. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Bromodeoxyuridine
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of BrdU antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/200 | 1/2000 | |
| Immunohistology - Paraffin |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit F(ab')2 anti Rat IgG:Dylight®405 | STAR16D405GA | F IF | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:DyLight®488 | STAR16D488GA | F IF | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
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