BrdU antibody | AbD33758kd

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Human anti BrdU

Product Type
Monoclonal Antibody
Clone
AbD33758kd
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
HCA322
Datasheet
F
MSDS
0.1 mg loader

Human anti BrdU antibody, clone AbD33758kd recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analogue, which is incorporated into the newly synthesized DNA during replication instead of thymidine.

Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

BrdU can be used as a thymidine analogue in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants.

Human anti BrdU antibody, clone AbD33758kd, has been tested in flow cytometry. Anti rat IgG2a specific antibodies are recommended as secondary detection reagents.

Product Details

Target Species
Chemical
Product Form
Recombinant chimeric human/rat IgG2a antibody selected from the HuCAL® phage display library and expressed in a human cell line. In this antibody, the VH and VL regions are human while the CH1-CH3 and C-lambda regions are derived from rat IgG2a. This antibody is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Bromodeoxyuridine
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim,
Soliris is a trademark of Alexion Pharmaceuticals, Inc.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of BrdU antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Rabbit F(ab')2 anti Rat IgG:Dylight®405 STAR16D405GA F IF 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:DyLight®488 STAR16D488GA F IF 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader