BrdU antibody | Bu20a
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Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used to label DNA synthesis in cultured mouse astrocytes by immunofluorescence.
Image caption:
Astrocyte response to injury involves proliferation which is attenuated by TTX and KB-R7943. (a) GFAP positive cultured rat cortical astrocytes (green) exhibit BrdU immunolabelling (red), seen in resting cells (top row) and scratched cells (second row). There is an increase in BrdU-positive cells along the edge of a scratch, which is attenuated with TTX and KB-R7943 treatment (third and fourth rows, respectively). Scale bar 200 μm. (b) After 24 h, there was a significant 77 ± 9% increase in proliferation amongst cells along the edge of a wound (n=7) compared to cells in unscratched cultures (n=8), as measured by BrdU staining. Compared to untreated cells, TTX decreased proliferation by 54 ± 4%, while KB-R7943 decreased proliferation by 46 ± 9%. *p<0.05; n.s., not significant.
From: Pappalardo LW, Samad OA, Black JA, Waxman SG.
Voltage-gated sodium channel Nav 1.5 contributes to astrogliosis in an in vitro model of glial injury via reverse Na+ /Ca2+ exchange.
Glia. 2014 Jul;62(7):1162-75.
> Visit our BrdU resources page.
Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.
Image caption:
Ghrelin enhances spatial pattern separation and adult hippocampal neurogenesis in adult rats.
(D) Representative images of DCX+ immature neurones and new adult-born DG neurones (white arrows) co-expressing NeuN+ and BrdU+ (yellow). Scale bar = 200μm.
From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.
The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 2015 Jan;51:431-9.
> Visit our BrdU resources page.
Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.
Image caption:
Ghrelin does not significantly inhibit the rate of stem cell self-renewal in the SGZ of the DG of adult rats. Representative images identifying triple positive (BrdU+/Sox2+/S100B+) new adult-born astrocytes (arrows) and double-positive (BrdU+/Sox2+/S100B−) new adult-born stem cells (arrowheads). Statistical analysis was performed using one-way ANOVA with Bonferroni's post hoc test, n = 12 rats per group. Scale bar = 20μm.
From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.
The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 2015 Jan;51:431-9.
> Visit our BrdU resources page.
Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used to stain dividing crypt cells in rat small intestine by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
BrdU/ Bu20a/ AbD serotec/ MCA2483, Small intestine/ Rat.
From: Furukawa S, Nagaike M, Ozaki K.
Databases for technical aspects of immunohistochemistry.
J Toxicol Pathol. 2017 Jan;30(1):79-107.
> Visit our BrdU resources page.
> Visit our BrdU resources page.
> Visit our BrdU resources page.
Mouse anti BrdU:FITC
Anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). This antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.
Mouse anti BrdU
Anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). This antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.
- Product Type
- Monoclonal Antibody
- Clone
- Bu20a
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA2483FA | F* | 50 µg | ||||
| MCA2483T | C F* IC* IF P | 20 µg | ||||
| MCA2483GA | C F* IC* IF P | 0.1 mg | ||||
| MCA2483 | C F* IC* IF P | 0.2 mg |
Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989).
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).
Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).
Product Details
- Target Species
- Chemical
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Bromodeoxyuridine conjugated to BSA
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of BrdU antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat | 1/10 | |
| Flow Cytometry 1 | 1/25 | 1/100 | |
| Immunocytochemistry 2 | 1/25 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
- 1 Flow Cytometry protocols can be found at:
www.bio-rad-antibodies.com/brdu-clone-bu20a-flow-cytometry-protocol
www.bio-rad-antibodies.com/brdu-staining-cell-cycle-protocol
- 1 Flow Cytometry protocols can be found at:
www.bio-rad-antibodies.com/brdu-clone-bu20a-flow-cytometry-protocol
www.bio-rad-antibodies.com/brdu-staining-cell-cycle-protocol - 2 BrdU labeling and immunostaining protocol can be found at:
www.bio-rad-antibodies.com/brdu-labeling-and-immunostaining-protocol
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) | STAR117D549GA | 0.1 mg | F IF WB | ||
| Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) | STAR117D649GA | 0.1 mg | F IF | ||
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | 0.5 mg | F | ||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | 1 ml | F | ||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | 1 mg | C E P RE WB | ||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | 1 mg | F |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| READIDROP™ Propidium Iodide | 1351101 | 3 X 3ml | F | ||
| PUREBLU™ DAPI | 1351303 | 250 µg | F IF | ||
| PUREBLU™ Hoechst 33342 | 1351304 | 280 µg | F IF |
Product Specific References
References for BrdU antibody
-
Magaud, J.P. et al. (1989) Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
J Histochem Cytochem. 37 (10): 1517-27. -
Innis, S.M. et al. (2010) Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.
Am J Physiol Gastrointest Liver Physiol. 299 (6): G1376-85. -
Caronia, G. et al. (2010) Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior.
J Neurosci. 30: 6291-301. -
Pappalardo, L.W. et al. (2014) Voltage-gated sodium channel Nav 1.5 contributes to astrogliosis in an in vitro model of glial injury via reverse Na+ /Ca2+ exchange.
Glia. 62 (7): 1162-75. -
Laitman, B.M. et al. (2016) The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination.
PLoS Biol. 14 (5): e1002467. -
Furukawa, S. et al. (2017) Databases for technical aspects of immunohistochemistry.
J Toxicol Pathol. 30 (1): 79-107. -
Wohl, S.G. et al. (2009) Optic nerve lesion increases cell proliferation and nestin expression in the adult mouse eye in vivo.
Exp Neurol. 219 (1): 175-86. -
Xie, L.L. et al. (2009) Aquaporin 4 knockout resists negative regulation of neural cell proliferation by cocaine in mouse hippocampus.
Int J Neuropsychopharmacol. 12 (6): 843-50. -
Miller, C. et al. (2011) The interplay between SUCLA2, SUCLG2, and mitochondrial DNA depletion.
Biochim Biophys Acta. 1812 (5): 625-9. -
Sato, Y. et al. (2013) Grafting of neural stem and progenitor cells to the hippocampus of young, irradiated mice causes gliosis and disrupts the granule cell layer.
Cell Death Dis. 4: e591. -
Kent BA et al. (2015) The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 51: 431-9. -
Li, Q. et al. (2017) Induced neural activity promotes an oligodendroglia regenerative response in the injured spinal cord and improves motor function after spinal cord injury.
J Neurotrauma. May 5. [Epub ahead of print] -
Kim, H.N. et al. (2017) Comparative analysis of the beneficial effects of treadmill training and electroacupuncture in a rat model of neonatal hypoxia-ischemia.
Int J Mol Med. 39 (6): 1393-402. -
Zhang, J. et al. (2017) The mechanisms underlying olfactory deficits in apolipoprotein E-deficient mice: focus on olfactory epithelium and olfactory bulb
Neurobiology of Aging. Oct 10 [Epub ahead of print].
