BrdU antibody | Bu20a

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Mouse anti BrdU:FITC

Anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). This antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.

Mouse anti BrdU

Anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). This antibody is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques.

Product Type
Monoclonal Antibody
Clone
Bu20a
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2483FA F* 50 µg
MCA2483 C F* IC* IF P 0.2 mg
MCA2483GA C F* IC* IF P 0.1 mg
MCA2483T C F* IC* IF P 20 µg
Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989).

Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).

BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014).

Product Details

Target Species
Chemical
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Bromodeoxyuridine conjugated to BSA
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of BrdU antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat 1/10
Flow Cytometry 1 1/25 1/100
Immunocytochemistry 2 1/25 1/100
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin
  1. 1 Flow Cytometry protocols can be found at:
    www.bio-rad-antibodies.com/brdu-clone-bu20a-flow-cytometry-protocol
    www.bio-rad-antibodies.com/brdu-staining-cell-cycle-protocol
  1. 1 Flow Cytometry protocols can be found at:
    www.bio-rad-antibodies.com/brdu-clone-bu20a-flow-cytometry-protocol
    www.bio-rad-antibodies.com/brdu-staining-cell-cycle-protocol
  2. 2 BrdU labeling and immunostaining protocol can be found at:
    www.bio-rad-antibodies.com/brdu-labeling-and-immunostaining-protocol
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
READIDROP™ Propidium Iodide 1351101 3 X 3ml F
PUREBLU™ DAPI 1351303 250 µg F IF
PUREBLU™ Hoechst 33342 1351304 280 µg F IF

Application Based External Images

Immunofluorescence

Immunohistology - Frozen

Product Specific References

References for BrdU antibody

  1. Magaud, J.P. et al. (1989) Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
    J Histochem Cytochem. 37 (10): 1517-27.
  2. Innis, S.M. et al. (2010) Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.
    Am J Physiol Gastrointest Liver Physiol. 299 (6): G1376-85.
  3. Caronia, G. et al. (2010) Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior.
    J Neurosci. 30: 6291-301.
  4. Pappalardo, L.W. et al. (2014) Voltage-gated sodium channel Nav 1.5 contributes to astrogliosis in an in vitro model of glial injury via reverse Na+ /Ca2+ exchange.
    Glia. 62 (7): 1162-75.
  5. Laitman, B.M. et al. (2016) The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination.
    PLoS Biol. 14 (5): e1002467.
  6. Furukawa, S. et al. (2017) Databases for technical aspects of immunohistochemistry.
    J Toxicol Pathol. 30 (1): 79-107.
  7. Wohl, S.G. et al. (2009) Optic nerve lesion increases cell proliferation and nestin expression in the adult mouse eye in vivo.
    Exp Neurol. 219 (1): 175-86.
  8. Xie, L.L. et al. (2009) Aquaporin 4 knockout resists negative regulation of neural cell proliferation by cocaine in mouse hippocampus.
    Int J Neuropsychopharmacol. 12 (6): 843-50.
  9. Miller, C. et al. (2011) The interplay between SUCLA2, SUCLG2, and mitochondrial DNA depletion.
    Biochim Biophys Acta. 1812 (5): 625-9.
  10. Sato, Y. et al. (2013) Grafting of neural stem and progenitor cells to the hippocampus of young, irradiated mice causes gliosis and disrupts the granule cell layer.
    Cell Death Dis. 4: e591.
  11. Kent BA et al. (2015) The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
    Psychoneuroendocrinology. 51: 431-9.
  12. Li, Q. et al. (2017) Induced neural activity promotes an oligodendroglia regenerative response in the injured spinal cord and improves motor function after spinal cord injury.
    J Neurotrauma. May 5. [Epub ahead of print]
  13. Kim, H.N. et al. (2017) Comparative analysis of the beneficial effects of treadmill training and electroacupuncture in a rat model of neonatal hypoxia-ischemia.
    Int J Mol Med. 39 (6): 1393-402.
  14. Zhang, J. et al. (2017) The mechanisms underlying olfactory deficits in apolipoprotein E-deficient mice: focus on olfactory epithelium and olfactory bulb
    Neurobiology of Aging. Oct 10 [Epub ahead of print].