BrdU antibody | Bu20a
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Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used to label DNA synthesis in cultured mouse astrocytes by immunofluorescence.
Image caption:
Astrocyte response to injury involves proliferation which is attenuated by TTX and KB-R7943. (a) GFAP positive cultured rat cortical astrocytes (green) exhibit BrdU immunolabelling (red), seen in resting cells (top row) and scratched cells (second row). There is an increase in BrdU-positive cells along the edge of a scratch, which is attenuated with TTX and KB-R7943 treatment (third and fourth rows, respectively). Scale bar 200 μm. (b) After 24 h, there was a significant 77 ± 9% increase in proliferation amongst cells along the edge of a wound (n=7) compared to cells in unscratched cultures (n=8), as measured by BrdU staining. Compared to untreated cells, TTX decreased proliferation by 54 ± 4%, while KB-R7943 decreased proliferation by 46 ± 9%. *p<0.05; n.s., not significant.
From: Pappalardo LW, Samad OA, Black JA, Waxman SG.
Voltage-gated sodium channel Nav 1.5 contributes to astrogliosis in an in vitro model of glial injury via reverse Na+ /Ca2+ exchange.
Glia. 2014 Jul;62(7):1162-75.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
> Visit our BrdU resources page.
Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.
Image caption:
Ghrelin enhances spatial pattern separation and adult hippocampal neurogenesis in adult rats.
(D) Representative images of DCX+ immature neurones and new adult-born DG neurones (white arrows) co-expressing NeuN+ and BrdU+ (yellow). Scale bar = 200μm.
From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.
The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 2015 Jan;51:431-9.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
> Visit our BrdU resources page.
Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used for the identification of proliferating cells in the rat dentate gyrus by immunofluorescence.
Image caption:
Ghrelin does not significantly inhibit the rate of stem cell self-renewal in the SGZ of the DG of adult rats. Representative images identifying triple positive (BrdU+/Sox2+/S100B+) new adult-born astrocytes (arrows) and double-positive (BrdU+/Sox2+/S100B−) new adult-born stem cells (arrowheads). Statistical analysis was performed using one-way ANOVA with Bonferroni's post hoc test, n = 12 rats per group. Scale bar = 20μm.
From: Kent BA, Beynon AL, Hornsby AK, Bekinschtein P, Bussey TJ, Davies JS, Saksida LM.
The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 2015 Jan;51:431-9.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
> Visit our BrdU resources page.
Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) used to stain dividing crypt cells in rat small intestine by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
BrdU/ Bu20a/ AbD serotec/ MCA2483, Small intestine/ Rat.
From: Furukawa S, Nagaike M, Ozaki K.
Databases for technical aspects of immunohistochemistry.
J Toxicol Pathol. 2017 Jan;30(1):79-107.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
> Visit our BrdU resources page.
> Visit our BrdU resources page.
> Visit our BrdU resources page.
Mouse anti Bromodeoxiuridine antibody, clone Bu20a (MCA2483) used for the identification of cells containing newly synthesized DNA in mouse brain and intestinal tissue sections by immunofluorescence.
Image caption:
Staining of mouse brain and intestine sections using MCA2483. A-C: A bright staining in the cell nuclei of individual cells scattered across the brain is present, especially in the hippocampus, ventricular wall (A) and sub-cortical areas at the border between the gray and white matters (B-C). Some positive cells are clearly M-phase cells (C). Abbreviations: GM = gray matter; LV = lateral ventricle; WM = white matter. DE: positive control staining in the intestine (duodenum). BrdU+ proliferating cells are clearly detected in the germinal layer of the intestinal epithelium, at the basis of the duodenal villi.
This image was generated in the lab of Prof. Merighi, Laboratory of Neurobiology, University of Turin, Italy. As a contribution to the Independent Validation Initiative under a Creative commons CC-BY-ND license.
Mouse anti BrdU antibody, clone Bu20a (MCA2483) used to identify proliferating cells in mouse brain sections by immunofluorescence.
Image caption:
NBP enhances arterial proliferation after ischemic stroke in vivo. Imaging examinations of αSMA-GFP and proliferation marker BrdU in the brain of 14 days after stroke. (A) Collateral arteries (arrows) and the ischemic region (*) can be visualized over the surface of the sensorimotor cortex. Each frame (dotted lines)=20X20 μm. (B) The confocal 3-D image shows vascular smooth muscle cells transgenically labeled with &apha;SMA-GFP (green) and stained for the proliferation marker BrdU (red) in the peri-infarct region. Those vascular cells of collateral arteries co-labeled with GFP and BrdU (yellow) were counted as newly formed vasculature. (C) and (D) Representative images from sham and stroke animals and quantified data of GFP/BrdU co-labeled cells in stroke animals with and without NBP treatment. *p<0.01 vs. sham, #p<0.05 vs. stroke + vehicle, N=6 in sham group, N=8 in stroke + vehicle and stroke + NBP groups, respectively. One-way ANOVA, F(2,14)=33.82. Bar=20 μm.
From: Wei ZZ, Chen D, Lee MJH, Zhao Y, Gu X, Yu SP, Wei L.
DL-3-n-butylphthalide Increases Collateriogenesis and Functional Recovery after Focal Ischemic Stroke in Mice.
Aging Dis. 2021 Oct 1;12(7):1835-1849.
doi: 10.14336/AD.2020.1226.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Bromodeoxyuridine antibody, clone Bu20a (MCA2483) usecd to indicate sites of de novoDNA synthesis in mouse brain by immunofluorescence.
Image caption:
Analysis of adult hippocampal neurogenesis in HMBS-deficient mice. b Quantification of hippocampal progenitor cell proliferation (BrdU+ cell count) and c respective fluorescence microscope images in HMBS-deficient (KI) and wildtype (WT) mice (BrdU+ cells in green).
From: Berger S, Stattmann M, Cicvaric A, Monje FJ, Coiro P, Hotka M, Ricken G, Hainfellner J, Greber-Platzer S, Yasuda M, Desnick RJ, Pollak DD.
Severe hydroxymethylbilane synthase deficiency causes depression-like behavior and mitochondrial dysfunction in a mouse model of homozygous dominant acute intermittent porphyria.
Acta Neuropathol Commun. 2020 Mar 20;8(1):38.
doi: 10.1186/s40478-020-00910-z.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti BrdU antibody, clone Bu20a (MCA2483) used to label dividing cells in mouse brain by immunofluorescence
Image caption:
A Diagrammatic representation of the experimental protocol. For the proliferation paradigm, mice were injected 4 times with BrdU, with a 2 h interval and euthanized 24 h after the last injection. For the survival paradigm, mice were injected twice daily, for 3 days and euthanized 14 days after the first injection. B Number of BrdU-positive cells in the proliferation paradigm, normalized to the size of the counted area. n = 8–9 mice/genotype (WT males:4; WT females: 4; USF-1 KO males:4; USF-1 KO females: 5). C Number of BrdU-positive cells in the survival paradigm, normalised to the size of the counted area. n = 5–6 mice/genotype (WT males:3; WT females: 2; USF-1 KO males:3; USF-1 KO females: 3). D Number of double-labelled BrdU and NeuN cells in the survival paradigm, normalised to the size of the counted area. n = 5–6 mice/genotype (WT males:3; WT females: 2; USF-1 KO males:3; USF-1 KO females: 3). D’ Representative microscopy image of a WT hippocampus; Red: BrdU; Green: NeuN; Blue: Dapi; scalebar: 500 µm. E Number of double-labelled BrdU and GFAP cells in the survival paradigm, normalised to the size of the counted area. n = 5–6 mice/genotype (WT males: 3; WT females: 2; USF-1 KO males:3; USF-1 KO females: 3). E’ Representative microscopy image of a WT hippocampus; Red: BrdU; Green: GFAP; Blue: Dapi; scalebar: 500 μm. Data are presented as mean ± SEM.
From: Sideromenos S, Nikou M, Czuczu B, Thalheimer N, Gundacker A, Horvath O, Cuenca Rico L, Stöhrmann P, Niello M, Partonen T, Pollak DD.
The metabolic regulator USF-1 is involved in the control of affective behaviour in mice.
Transl Psychiatry. 2022 Dec 1;12(1):497.
doi: 10.1038/s41398-022-02266-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti BrdU antibody, clone Bu20a (MCA2483) used to identify BrdU incorporating cells in mouse brain by immunofluorescence.
Image caption:
Adult hippocampal neurogenesis is not altered upon after early-life ID . (B) Representative images of BrdU staining in offspring of mothers provided with an iron-deficient diet (IDD ) (48 ppm) or iron-sufficient diet (ISD ) (96 ppm) during pregnancy and lactation (until postnatal day 10). Scale bars: 200 μm, 20x magnification. (C) Quantification of BrdU+ cells in the hippocampus (n=5–9/group).
From: Gundacker A, Glat M, Wais J, Stoehrmann P, Pollak A, Pollak DD.
Early-life iron deficiency persistently disrupts affective behaviour in mice.
Ann Med. 2023 Dec;55 (1):1265-77.
doi: 10.1080/07853890.2023.2191003.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti BrdU
- Product Type
- Monoclonal Antibody
- Clone
- Bu20a
- Isotype
- IgG1
- Specificity
- BrdU
Antibody Quality and Performance Guarantee
| Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which is incorporated to new DNA during replication instead of thymidine. BrdU can therefore be used to identify newly synthesized DNA. Mouse anti BrdU antibody, clone Bu20a, recognizes BrdU and other thymidine analogs; 5′-chloro-2′-deoxyuridine (CldU), 5′-iodo-2′-deoxyuridine (IdU) and 2′-deoxy-5-ethynyluridine (EdU), but only shows minimal reactivity with thymidine itself (Aten et al. 1992, Liboska et al. 2012, Magaud et al. 1989). Antibody detection of incorporated BrdU in cellular DNA is extensively referenced as an accurate method to monitor cell proliferation in vivo and in vitro. In cell proliferation assays BrdU staining is coupled with the use of a dye that binds total DNA such as propidium iodide (PI). BrdU can be administered diluted in the culture medium or, in vivo via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004). BrdU can be used as a thymidine analog in a wide range of organisms ranging from mammalian cells, through reptiles and amphibians to invertebrate species and plants. Mouse anti BrdU antibody, clone Bu20a, is suitable for detecting incorporated BrdU in a wide variety of cell types and is suitable for use on tissue sections in double-labeling techniques (Makarev and Gorivodsky 2014). |
- Target Species
- Chemical
- Product Form
- Purified IgG - liquid
- Preparation
- MCA2483T: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- MCA2483GA, MCA2483: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Immunogen
- Bromodeoxyuridine conjugated to BSA
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the NS1 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/25 | 1/100 |
| Immunocytochemistry 2 | |
1/25 | 1/100 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
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| Immunohistology - Paraffin | |
- 1 Flow Cytometry protocols can be found at:
www.bio-rad-antibodies.com/brdu-clone-bu20a-flow-cytometry-protocol
www.bio-rad-antibodies.com/brdu-staining-cell-cycle-protocol - 2 BrdU labeling and immunostaining protocol can be found at:
www.bio-rad-antibodies.com/brdu-labeling-and-immunostaining-protocol
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100 μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | STAR117F | F | 0.5 mg |
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| Description | Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:RPE | STAR12A | F | 1 ml |
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| Description | Rabbit F(ab')2 anti Mouse IgG:RPE | ||||||
| Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | STAR13B | C E P RE WB | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) | ||||||
| Rabbit F(ab')2 anti Mouse IgG:FITC | STAR9B | F | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:FITC | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Magaud, J.P. et al. (1989) Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.
J Histochem Cytochem. 37 (10): 1517-27.
References for BrdU antibody
-
Xie, L.L. et al. (2009) Aquaporin 4 knockout resists negative regulation of neural cell proliferation by cocaine in mouse hippocampus.
Int J Neuropsychopharmacol. 12 (6): 843-50. -
Wohl, S.G. et al. (2009) Optic nerve lesion increases cell proliferation and nestin expression in the adult mouse eye in vivo.
Exp Neurol. 219 (1): 175-86. -
Innis, S.M. et al. (2010) Perinatal lipid nutrition alters early intestinal development and programs the response to experimental colitis in young adult rats.
Am J Physiol Gastrointest Liver Physiol. 299 (6): G1376-85. -
Caronia, G. et al. (2010) Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior.
J Neurosci. 30: 6291-301. -
Miller, C. et al. (2011) The interplay between SUCLA2, SUCLG2, and mitochondrial DNA depletion.
Biochim Biophys Acta. 1812 (5): 625-9. -
Sato, Y. et al. (2013) Grafting of neural stem and progenitor cells to the hippocampus of young, irradiated mice causes gliosis and disrupts the granule cell layer.
Cell Death Dis. 4: e591. -
Pappalardo, L.W. et al. (2014) Voltage-gated sodium channel Nav 1.5 contributes to astrogliosis in an in vitro model of glial injury via reverse Na+ /Ca2+ exchange.
Glia. 62 (7): 1162-75. -
Kent BA et al. (2015) The orexigenic hormone acyl-ghrelin increases adult hippocampal neurogenesis and enhances pattern separation.
Psychoneuroendocrinology. 51: 431-9.
View The Latest Product References
-
Laitman, B.M. et al. (2016) The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination.
PLoS Biol. 14 (5): e1002467. -
Li, Q. et al. (2017) Induced Neural Activity Promotes an Oligodendroglia Regenerative Response in the Injured Spinal Cord and Improves Motor Function after Spinal Cord Injury.
J Neurotrauma. 34 (24): 3351-61. -
Kim, H.N. et al. (2017) Comparative analysis of the beneficial effects of treadmill training and electroacupuncture in a rat model of neonatal hypoxia-ischemia.
Int J Mol Med. 39 (6): 1393-402. -
Furukawa, S. et al. (2017) Databases for technical aspects of immunohistochemistry.
J Toxicol Pathol. 30 (1): 79-107. -
Zhang, J. et al. (2018) The mechanisms underlying olfactory deficits in apolipoprotein E-deficient mice: focus on olfactory epithelium and olfactory bulb.
Neurobiol Aging. 62: 20-33. -
Berger, S. et al. (2020) Severe hydroxymethylbilane synthase deficiency causes depression-like behavior and mitochondrial dysfunction in a mouse model of homozygous dominant acute intermittent porphyria.
Acta Neuropathol Commun. 8 (1): 38. -
Wei, Z.Z. et al. (2021) DL-3-n-butylphthalide Increases Collateriogenesis and Functional Recovery after Focal Ischemic Stroke in Mice.
Aging Dis. 12 (7): 1835-49. -
Lee, H.J. et al. (2022) Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke
Neural Regeneration Research. 17 (7): 1556. -
Sideromenos, S. et al. (2022) The metabolic regulator USF-1 is involved in the control of affective behaviour in mice.
Transl Psychiatry. 12 (1): 497. -
Gundacker, A. et al. (2023) Early-life iron deficiency persistently disrupts affective behaviour in mice.
Ann Med. 55 (1): 1265-77.
- Synonyms
- 5-BROMODEOXYURIDINE
- RRID
- AB_1102864
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