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GAPDH antibody

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Anti GAPDH (N-Terminal) Antibody thumbnail image 1 Anti GAPDH (N-Terminal) Antibody thumbnail image 2 Anti GAPDH (N-Terminal) Antibody thumbnail image 3 Anti GAPDH (N-Terminal) Antibody thumbnail image 4 Anti GAPDH (N-Terminal) Antibody thumbnail image 5 Anti GAPDH (N-Terminal) Antibody thumbnail image 6 Anti GAPDH (N-Terminal) Antibody thumbnail image 7
Anti GAPDH (N-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from HeLa, human epithelial adenocarcinoma cells probed with Rabbit anti Human GAPDH (AHP1628) at 0.125(A), 0.25(B) and 0.5(C) ug/ml.
Anti GAPDH (N-Terminal) Antibody gallery image 2
Immunohistochemical staining of human liver with Rabbit anti Human GAPDH (AHP1628).
Anti GAPDH (N-Terminal) Antibody gallery image 3
HeLa (lane 2) and HepG2 (lane 3) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lane 1. Rabbit anti Human GAPDH (AHP1628) was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP MCA6003P was used as the secondary antibody at a dilution of 1/5000 . Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure
Anti GAPDH (N-Terminal) Antibody gallery image 4
Multiplex staining of HeLa whole cell lysate with Mouse anti PCNA (MCA1558) detected with Goat anti Mouse IgG (H/L):DyLight®680 (STAR117D680GA) shown in red and Rabbit anti GAPDH (AHP1628) detected with Sheep anti Rabbit IgG:DyLight®488 (STAR36D488GA) shown in green
Anti GAPDH (N-Terminal) Antibody gallery image 5
HeLa cells were treated with 10 µg BrdU for 1 hour (B) or left untreated (A). Cells were stained with mouse anti-BrdU antibody, clone Bu20a (MCA2483) at a dilution of 1/25. As a secondary antibody, goat anti mouse IgG (H/L) DyLight® 549 conjugated antibody (red) (STAR117D549GA) was used at a 1/50 dilution. Cytoplasm was stained with rabbit anti-GAPDH antibody (AHP1628) at a dilution of 1/100. As a secondary antibody, sheep anti rabbit IgG DyLight® 488 conjugated antibody (green) (STAR36D488GA) was used at a 1/50 dilution. PureBlu™ DAPI (1351303) was used as nuclear counterstain.
Anti GAPDH (N-Terminal) Antibody gallery image 6
Published customer image:
Rabbit anti GAPDH antibody (AHP1628) used to evaluate GAPDH expression in murine tissue lysates by western blotting.
Image caption:
NUCB2 is expressed in the small intestine, large intestine and liver of male C57BL/6 mice. Tissues show distinct bands for NUCB2/nesfatin-1 corresponding to 50 kDa. Rat nesfatin-1 (Custom synthesized, ABGENT, 1 μg/μL) was used as a positive control which is shown as a distinct band corresponding to 10 kDa (A; Left Image). Mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is uniformly expressed in liver, small and large intestine. The blots that were loaded with 50 μg of total protein/well previously for NUCB2 protein detection were stripped and re-blocked with mouse GAPDH primary antibody. Representative blot (n = 4 independent experiments) showing distinct bands for GAPDH corresponding to 37 kDa (A; Right Image). Mucosal cells showing NUCB2/nesfatin-1 (Texas-Red) immunoreactivity, and DAPI are found in the small intestine (B; left image) and large intestine (B; right image) from male mice. Arrows point to cells that are immunopositive for NUCB2/nesfatin-1 and shows distinct DAPI-stained nuclei. Representative images were taken of 4 slides (8 sections/tissue) from three adult male mice. Scale bar = 100μm.

From: Mohan H, Ramesh N, Mortazavi S, Le A, Iwakura H, Unniappan S (2014)
Nutrients Differentially Regulate Nucleobindin-2/Nesfatin-1 In Vitro in Cultured Stomach Ghrelinoma (MGN3-1) Cells and In Vivo in Male Mice.
PLoS ONE 9(12): e115102.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti GAPDH (N-Terminal) Antibody gallery image 7
HeLa cells were treated with BrdU for 3 hours and stained with mouse anti-BrdU antibody, clone Bu20a (MCA2483). Goat anti mouse IgG (H/L) DyLight® 549 conjugated antibody (red) (STAR117D549GA) was used as the secondary antibody. Cytoplasm was stained with rabbit anti-GAPDH antibody (AHP1628). As the secondary antibody, sheep anti rabbit IgG DyLight® 488 conjugated antibody (green) (STAR36D488GA) was used. PureBlu™ DAPI (1351303) was used as nuclear counterstain (blue)

Rabbit anti GAPDH (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP1628T
Datasheet
IC P* WB
SDS
50 µg loader

AHP1628
Datasheet
IC P* WB
SDS
0.1 mg loader

Rabbit anti GAPDH (N-Terminal) antibody recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is a major glycolytic enzyme within the cytosol, which is also involved in a number of intracellular processes including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair.

GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human GADPH (N-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to a 16 amino acid sequence from near the amino-terminus of GAPDH.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P04406
Entrez Gene
GAPDH
GO Terms
GO:0005515 protein binding
GO:0016020 membrane
GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006094 gluconeogenesis
GO:0006096 glycolysis
GO:0055114 oxidation-reduction process
GO:0050821 protein stabilization
GO:0035605 peptidyl-cysteine S-nitrosylase activity
GO:0035606 peptidyl-cysteine S-trans-nitrosylation
GO:0048471 perinuclear region of cytoplasm
GO:0051287 NAD binding
GO:0051402 neuron apoptosis
Regulatory
For research purposes only

Applications of GAPDH antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunocytochemistry 1/25 1/100
Immunohistology - Paraffin 1 10ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human liver tissue
Western Blotting
AHP1628 detects a band of approximately 40kDa in HeLa cell lysates.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Histar Detection System STAR3000A C P 50 Tests
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
Antigen Retrieval Buffer, pH8.0 BUF025C P 50 ml
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for GAPDH antibody

  1. Rogalska, A. et al. (2014) Epothilone B induces extrinsic pathway of apoptosis in human SKOV-3 ovarian cancer cells.
    Toxicol In Vitro. 28: 675-83.
  2. Paré, B. et al. (2015) Early detection of structural abnormalities and cytoplasmic accumulation of TDP-43 in tissue-engineered skins derived from ALS patients.
    Acta Neuropathol Commun. 3 (1): 5.
  3. Mohan, H. et al. (2014) Nutrients differentially regulate nucleobindin-2/nesfatin-1 in vitro in cultured stomach ghrelinoma (MGN3-1) cells and in vivo in male mice.
    PLoS One. 9 (12): e115102.
  4. Nevzorova, Y.A. et al. (2017) Anti-tumorigenic and anti-angiogenic effects of natural conifer Abies sibirica terpenoids in vivo and in vitro.
    Biomed Pharmacother. 89: 386-95.
  5. Antonucci, J.M. et al. (2016) SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.
    Nat Med. 22 (10): 1072-1074.
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