GAPDH antibody
Rabbit anti GAPDH antibody (AHP1628) used to evaluate GAPDH expression in murine tissue lysates by western blotting.
Image caption:
NUCB2 is expressed in the small intestine, large intestine and liver of male C57BL/6 mice. Tissues show distinct bands for NUCB2/nesfatin-1 corresponding to 50 kDa. Rat nesfatin-1 (Custom synthesized, ABGENT, 1 μg/μL) was used as a positive control which is shown as a distinct band corresponding to 10 kDa (A; Left Image). Mouse glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is uniformly expressed in liver, small and large intestine. The blots that were loaded with 50 μg of total protein/well previously for NUCB2 protein detection were stripped and re-blocked with mouse GAPDH primary antibody. Representative blot (n = 4 independent experiments) showing distinct bands for GAPDH corresponding to 37 kDa (A; Right Image). Mucosal cells showing NUCB2/nesfatin-1 (Texas-Red) immunoreactivity, and DAPI are found in the small intestine (B; left image) and large intestine (B; right image) from male mice. Arrows point to cells that are immunopositive for NUCB2/nesfatin-1 and shows distinct DAPI-stained nuclei. Representative images were taken of 4 slides (8 sections/tissue) from three adult male mice. Scale bar = 100μm.
From: Mohan H, Ramesh N, Mortazavi S, Le A, Iwakura H, Unniappan S (2014)
Nutrients Differentially Regulate Nucleobindin-2/Nesfatin-1 In Vitro in Cultured Stomach Ghrelinoma (MGN3-1) Cells and In Vivo in Male Mice.
PLoS ONE 9(12): e115102.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rabbit anti GAPDH (N-Terminal) antibody recognizes human glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is a major glycolytic enzyme within the cytosol, which is also involved in a number of intracellular processes including membrane fusion, microtubule bundling, phosphotransferase, DNA replication and DNA repair.
GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease.
GAPDH may be involved in the cellular phenotype of age-related neurodegenerative disorders such as Alzheimer’s and Huntington’s disease.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human GADPH (N-Terminal) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- A peptide corresponding to a 16 amino acid sequence from near the amino-terminus of GAPDH.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P04406
- Entrez Gene
- GAPDH
- GO Terms
- GO:0005515 protein binding
- GO:0016020 membrane
- GO:0004365 glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity
- GO:0005829 cytosol
- GO:0005634 nucleus
- GO:0006094 gluconeogenesis
- GO:0006096 glycolysis
- GO:0055114 oxidation-reduction process
- GO:0050821 protein stabilization
- GO:0035605 peptidyl-cysteine S-nitrosylase activity
- GO:0035606 peptidyl-cysteine S-trans-nitrosylation
- GO:0048471 perinuclear region of cytoplasm
- GO:0051287 NAD binding
- GO:0051402 neuron apoptosis
- Regulatory
- For research purposes only
Applications of GAPDH antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunocytochemistry | 1/25 | 1/100 | |
| Immunohistology - Paraffin 1 | 10ug/ml | ||
| Western Blotting | 0.5 | 1.0ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human liver tissue
- Western Blotting
- AHP1628 detects a band of approximately 40kDa in HeLa cell lysates.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
|
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| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
|
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| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
|
|
| Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
|
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| Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
|
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| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Antigen Retrieval Buffer, pH8.0 | BUF025A | P | 500 ml | ||
| 100x Antigen Retrieval Buffer, pH8.0 | BUF025C | P | 50 ml | ||
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for GAPDH antibody
-
Rogalska, A. et al. (2014) Epothilone B induces extrinsic pathway of apoptosis in human SKOV-3 ovarian cancer cells.
Toxicol In Vitro. 28: 675-83. -
Paré, B. et al. (2015) Early detection of structural abnormalities and cytoplasmic accumulation of TDP-43 in tissue-engineered skins derived from ALS patients.
Acta Neuropathol Commun. 3 (1): 5. -
Mohan, H. et al. (2014) Nutrients differentially regulate nucleobindin-2/nesfatin-1 in vitro in cultured stomach ghrelinoma (MGN3-1) cells and in vivo in male mice.
PLoS One. 9 (12): e115102. -
Nevzorova, Y.A. et al. (2017) Anti-tumorigenic and anti-angiogenic effects of natural conifer Abies sibirica terpenoids in vivo and in vitro.
Biomed Pharmacother. 89: 386-95. -
Antonucci, J.M. et al. (2016) SAMHD1-mediated HIV-1 restriction in cells does not involve ribonuclease activity.
Nat Med. 22 (10): 1072-1074.
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