PUREBLU™ DAPI

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PUREBLU™ DAPI gallery image 1

HeLa cells, formaldehyde fixed and permeabilised, stained with rat anti alpha tubulin (red) (MCA78G), mouse anti human CD49a (green) (MCA1133) and PureBlu DAPI (blue) (135-1303)

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PUREBLU™ DAPI gallery image 2

Parafin fixed human pancreas stained with guinea pig anti insulin (red) (5330-0104G), mouse anti chromogranin A (green) (MCA4773) and PureBlu DAPI (blue) (135-1303)

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PUREBLU™ DAPI gallery image 3

Paraffin section of human colon adenocarcinoma (HIER citrate pH6 antigen retrieval) blocked with 10% FCS and stained with mouse anti human cytokeratin 18 (MCA1864H) and detected with goat anti mouse IgG:A488 (STAR117D488GA). The tissue was also counterstained with PureBlu DAPI (135-1303)

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  • PUREBLU™ DAPI thumbnail image 1
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  • PUREBLU™ DAPI
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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    1351303IFdatasheet pdfdatasheet pdf250 µg
    1351303
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • DAPI is a cell permeable fluorescent compound that is able to stain the DNA of eukaryotic and prokaryotic cells by binding with high affinity to the minor groove of AT-rich DNA sequences. When DAPI is bound to DNA and excited by an ultraviolet light source, blue fluorescent emission can be detected with maximum emission at at 461 nm. PureBlu DAPI has a characteristic Stokes shift of approximately 100 nm, which makes this dye an optimal choice when good spectral separation is desired. PureBlu DAPI is compatible with fixed and unfixed cells.
    • Intended Use
    • Product Form
    • Reconstitution
      1. Reconstitute one vial of lyophilized PureBlu DAPI Dye with 500 μl of de-ionized water, then vortex briefly to make 100x stock solution.

      2. Dilute the 100x stock solution 1:100 with 1x phosphate buffered saline to make a 1 μg/ml staining solution.
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Molecular Weight
      350.3
    • Reagents In The Kit
      5 vials, 50 μg each, DAPI nuclear staining dye powder
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at - 20oC
      After reconstitution store at +4oC or at -20oC if preferred
      This product is photosensitive and should be protected from light. PureBlu™ DAPI Dye is stable for 12 months from date of reconstitution if stored at - 20oC or 6 months at 2-8oC.
    • Shelf Life
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunofluorescence1/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Note: The optimal concentration for different cell types should be determined empirically.

      1. Culture cells of interest under appropriate conditions.

      2. Rinse cells with 1x phosphate buffered saline.

      3. Optional: Fix cells with 3.7% formaldehyde at room temperature (18-25oC) for 10 minutes.

      4. Optional: Rinse cells with 1x phosphate buffered saline and permeabilize with 0.1% Triton x-100 in phosphate buffered saline at room temperature (18-25oC) for 5 minutes.

      5. Rinse cells with 1x phosphate buffered saline.

      6. Stain with 1x staining solution at room temperature (18-25oC) for 15 minutes.

      7. Rinse cells with 1x phosphate buffered saline.

      8. Optional: Remove phosphate buffered saline and mount cells in antifade-mounting media.

      9. Image cells.



      Triton is a trademark of Dow Chemical Company.

    Additional PUREBLU™ DAPI Formats

    Formats Applications Sizes available
    PUREBLU™ DAPI : Reagent IF 250 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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