PUREBLU™ DAPI





Product Details
- Reconstitution
- 1. Reconstitute one vial of lyophilized PureBlu DAPI Dye with 500 μl of de-ionized water, then vortex briefly to make 100x stock solution.
2. Dilute the 100x stock solution 1:100 with 1x phosphate buffered saline to make a 1 μg/ml staining solution. - Reagents in the Kit
- 5 vials, 50 μg each, DAPI nuclear staining dye powder
Storage Information
- Storage
- Prior to reconstitution store at - 20oC
After reconstitution store at +4oC or at -20oC if preferred
This product is photosensitive and should be protected from light. PureBlu™ DAPI Dye is stable for 12 months from date of reconstitution if stored at - 20oC or 6 months at 2-8oC. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of PUREBLU™ DAPI
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/500 | ||
Immunofluorescence | 1/500 |
- Instructions For Use
- Note: The optimal concentration for different cell types should be determined empirically.
1. Culture cells of interest under appropriate conditions.
2. Rinse cells with 1x phosphate buffered saline.
3. Optional: Fix cells with 3.7% formaldehyde at room temperature (18-25oC) for 10 minutes.
4. Optional: Rinse cells with 1x phosphate buffered saline and permeabilize with 0.1% Triton x-100 in phosphate buffered saline at room temperature (18-25oC) for 5 minutes.
5. Rinse cells with 1x phosphate buffered saline.
6. Stain with 1x staining solution at room temperature (18-25oC) for 15 minutes.
7. Rinse cells with 1x phosphate buffered saline.
8. Optional: Remove phosphate buffered saline and mount cells in antifade-mounting media.
9. Image cells.
Triton is a trademark of Dow Chemical Company.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra