Panitumumab antibody | AbD23895_hIgG1
A microtiter plate was coated over night with various antigens at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23895_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®
A microtiter plate was coated over night with panitumumab, human IgG2/kappa, adalimumab or human EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti panitumumab (clone AbD23895_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®.Data for binding to panitumumab is shown as the mean of three measurements
A microtiter plate was coated over night with Human anti panitumumab (clone AbD23894, HCA264) at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of panitumumab. Detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23895_hIgG1, HCA266) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®
A microtitre plate was coated over night with panitumumab at a concentration of 1 μg/ml (black line) or 0.5 μg/ml (red line). After washing and blocking with PBST+5% BSA, 10% Human serum was added spiked with increasing concentrations of Human anti panitumumab HCA265 (clone AbD23897_hIgG1) or HCA266 (clone AbD23895_hIgG1). Detection was performed using HRP-conjugated panitumumab. Data is shown as the mean of three measurements
A microtiter plate was coated over night with EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of panitumumab (0.3 μg/ml) plus increasing concentrations of Human anti panitumumab (clone AbD23895) in the monovalent Fab format was added. Free panitumumab, still capable of binding to the human EGFR coated plate, was detected using Goat anti Human IgG F(ab')2 HRP (0500-0099) labeled antibody and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements
Human anti Panitumumab
Anti-panitumumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in fully human IgG1 format; it is ideal for bioanalytical assays for panitumumab and biosimilars. It can be used as the detection antibody in a PK bridging ELISA and as a reference standard in an ADA bridging ELISA.
- Product Type
- Monoclonal Antibody
- Clone
- AbD23895_hIgG1
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA266 | E | 0.1 mg |
|
Human anti panitumumab clone AbD23895_hIgG1, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the fully human monoclonal antibody drug panitumumab and inhibits it binding to its target, epidermal growth factor receptor (EGFR). The antibody can be used to measure the levels of panitumumab and biosimilar products in bioanalytical assays.
Clone AbD23895_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD23894 (HCA264) in monovalent Fab format, as the capture antibody.
Panitumumab (Vectibix®) is a fully human monoclonal antibody (IgG2/kappa) specific to the transmembrane protein EGFR. The drug binds to the extracellular domain of the EGFR, inhibiting the activation of the intracellular signalling cascade. It is approved by the FDA and EMA for the treatment of EGFR-expressing metastatic colorectal cancer.
View a summary of all anti panitumumab antibodies
Clone AbD23895_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD23894 (HCA264) in monovalent Fab format, as the capture antibody.
Panitumumab (Vectibix®) is a fully human monoclonal antibody (IgG2/kappa) specific to the transmembrane protein EGFR. The drug binds to the extracellular domain of the EGFR, inhibiting the activation of the intracellular signalling cascade. It is approved by the FDA and EMA for the treatment of EGFR-expressing metastatic colorectal cancer.
View a summary of all anti panitumumab antibodies
Product Details
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL® phage display library and expressed in a human cell line.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Panitumumab
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD = 0.6 nM by real time, label free molecular interaction analysis on immobilized panitumumab.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Panitumumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in direct or indirect ELISA and in an ELISA bridging assay as a detection antibody together with HCA264 as the capture reagent. It is also suitable as a fully human control or calibrator antibody in an ADA bridging ELISA.
Protocol: PK bridging ELISA to measure free drug
Protocol: ADA bridging ELISA
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Panitumumab | HCA264 | E | 0.1 mg |
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| Human anti Panitumumab | HCA265 | E | 0.1 mg |
|
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| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
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