Panitumumab antibody | AbD23895_hIgG1
A microtiter plate was coated over night with various antigens at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23895_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.
A microtiter plate was coated over night with panitumumab, human IgG2/kappa, adalimumab or human EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti panitumumab (clone AbD23895_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu fluorogenic peroxidase substrate. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit.Data for binding to panitumumab is shown as the mean of three measurements.
A microtiter plate was coated over night with Human anti panitumumab (clone AbD23894, HCA264) at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of panitumumab. Detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23895_hIgG1, HCA266) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®
A microtitre plate was coated over night with panitumumab at a concentration of 1 μg/ml (black line) or 0.5 μg/ml (red line). After washing and blocking with PBST+5% BSA, 10% Human serum was added spiked with increasing concentrations of Human anti panitumumab HCA265 (clone AbD23897_hIgG1) or HCA266 (clone AbD23895_hIgG1). Detection was performed using HRP-conjugated panitumumab. Data is shown as the mean of three measurements.
A microtiter plate was coated over night with EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of panitumumab (0.3 μg/ml) plus increasing concentrations of Human anti panitumumab (clone AbD23895) in the monovalent Fab format was added. Free panitumumab, still capable of binding to the human EGFR coated plate, was detected using Goat anti Human IgG F(ab')2 HRP (0500-0099) labeled antibody and QuantaBlu fluorogenic peroxidase substrate. Data is shown as the mean of three measurements.
Filter by Application:
E ResetHuman anti Panitumumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD23895_hIgG1
- Isotype
- IgG1
- Specificity
- Panitumumab
Antibody Quality and Performance Guarantee
| Human anti panitumumab clone AbD23895_hIgG1, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the fully human monoclonal antibody drug panitumumab and inhibits it binding to its target, epidermal growth factor receptor (EGFR). The antibody can be used to measure the levels of panitumumab and biosimilar products in bioanalytical assays. Clone AbD23895_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD23894 (HCA264) in monovalent Fab format, as the capture antibody. Panitumumab (Vectibix®) is a fully human monoclonal antibody (IgG2/kappa) specific to the transmembrane protein EGFR. The drug binds to the extracellular domain of the EGFR, inhibiting the activation of the intracellular signalling cascade. It is approved by the FDA and EMA for the treatment of EGFR-expressing metastatic colorectal cancer. View a summary of all anti panitumumab antibodies |
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal.
- Immunogen
- Panitumumab
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD = 0.6 nM as measured by real time, label free molecular interaction analysis on immobilized Panitumumab.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in direct or indirect ELISA and in an ELISA bridging assay as a detection antibody together with HCA264 as the capture reagent. It is also suitable as a fully human control or calibrator antibody in an ADA bridging ELISA.
Protocol: PK bridging ELISA to measure free drug.
Protocol: ADA bridging ELISA.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Human IgG F(ab')2:HRP | 0500-0099 | E WB | 0.5 ml |
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| Description | Goat anti Human IgG F(ab')2:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
| List Price | Your Price | ||||||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Panitumumab | HCA264 | E | 0.1 mg |
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| Description | Human anti Panitumumab | ||||||
| Human anti Panitumumab | HCA265 | E | 0.1 mg |
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| Description | Human anti Panitumumab | ||||||
- Synonyms
- VECTIBIX®
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA266
1511
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