Human IgG4 | AbD18705_hIgG4

Recombinant Human IgG4 Kappa

Product Type
Recombinant Protein
Clone
AbD18705_hIgG4
Isotype
IgG4 Kappa
Specificity
Human IgG4
Region
KAPPA

Product Code Applications Pack Size List Price Your Price Qty
HCA195
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
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loader
HCA195G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
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loader
Search for Batch Specific Datasheets

Recombinant Human IgG4 kappa, AbD18705_hIgG4 is a recombinant human IgG4 antibody with a kappa light chain.

AbD18705_hIgG4 is specific for green fluorescent protein (GFP) and has no known reactivity with mammalian proteins or other antigens. This product is recommended for use as a standard in assays designed to measure IgG4 levels, or as a control antibody when using other human antibodies of the same isotype and subclass.

In addition to the tetrameric IgG structure, composed of two copies each of the heavy and light chains, human IgG4 forms so-called half-molecules. This species contains only one heavy and one light chain. Half-molecules can assemble with an unrelated half-molecule to form bispecific tetrameric antibody species (Aalberse et al. 2002). Half-molecules of human IgG4 preparations can be detected as an additional species under denaturing, non-reducing conditions using SDS-PAGE, size exclusion chromatography or capillary electrophoresis.

Mutation in the core hinge region of IgG4 prevents the formation of half-molecules (Bloom et al. 1997). In addition to the wild type recombinant, Bio-Rad also offers recombinant Human IgG4 kappa in its mutated form (HCA247). Recombinant Human IgG4 lambda is also available in both wild type (HCA050) and mutant form (HCA246).

Product Form
Human IgG4 antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
HCA195: Purified IgG prepared by affinity chromatography on Protein A
HCA195G: Purified IgG prepared by affinity chromatography on Protein A.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Green fluorescent protein.
Approx. Protein Concentrations
Pack Size: 0.1 mg
Antibody concentration 0.5 mg/ml
Pack Size: 1 mg
Antibody concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

Description Product Code Applications Pack Size List Price Your Price Quantity
Recombinant Human IgG4 Lambda HCA050 E 0.1 mg loader
List Price Your Price
loader
Description Recombinant Human IgG4 Lambda
Recombinant Human IgG4 Lambda (Mutant) HCA246 E 0.1 mg loader
List Price Your Price
loader
Description Recombinant Human IgG4 Lambda (Mutant)
Recombinant Human IgG4 Kappa (Mutant) HCA247 E 0.1 mg loader
List Price Your Price
loader
Description Recombinant Human IgG4 Kappa (Mutant)

Further Reading

  1. Aalberse R.C. et. al. (2002) IgG4 breaking the rules.
    Immunology 105:9-19
  2. Bloom J.W. et. al. (1997) Intrachain disulfide bond in the core hinge region of human IgG4.
    Protein Sci. 1997 Feb;6(2):407-15

UniProt
P01834
P01861
Entrez Gene
IGKC
IGHG4
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0005624 membrane fraction
GO:0006955 immune response
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"