CD8 Beta antibody | PPT23

Mouse anti Pig CD8 Beta Chain

Product Type
Monoclonal Antibody
Clone
PPT23
Isotype
IgG1
Specificity
CD8 Beta

Product Code Applications Pack Size List Price Your Price Qty
MCA5954GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Pig CD8 beta chain, clone PPT23, recognizes the porcine homologue of the human CD8 beta chain cell surface antigen.

Characterization of clone PPT23, also known under the clone designation FYP1C5, has demonstrated that on peripheral blood lymphocytes and spleen, this antibody binds to cells that are CD3+, CD4-, CD8hi and as such defines this antibody as a specific marker of porcine α/β T cells, in thymic tissue both CD8lo and CD8hi cells are recognized (Yang & Parkhouse 1997). Mouse anti Pig CD8 beta chain, clone PPT23 recognizes a different epitope on the CD8 beta chain to clone PPT22.

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Porcine thymus membrane lysate
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Lymph node cells from immunized BALB/c mice were fused with cells of the NSO myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

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References for CD8 Beta antibody

  1. Yang, H. & Parkhouse, R.M. (1997) Differential expression of CD8 epitopes amongst porcine CD8-positive functional lymphocyte subsets.
    Immunology. 92 (1): 45-52.
  2. Mair, K.H. et al. (2015) Carbopol improves the early cellular immune responses induced by the modified-life vaccine Ingelvac PRRS® MLV.
    Vet Microbiol. 176 (3-4): 352-7.
  3. Sinkora, M. et al. (2007) Two groups of porcine TCRgammadelta+ thymocytes behave and diverge differently.
    J Immunol. 178 (2): 711-9.

Further Reading

  1. Zuckermann, F.A. et al. (1998) Report on the analyses of mAb reactive with porcine CD8 for the second international swine CD workshop.
    Vet Immunol Immunopathol. 60 (3-4): 291-303.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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