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IgG antibody

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Anti Mouse IgG (Rat Adsorbed) Ab - RPE gallery image 1
Figure A. FITC conjugated Rat anti Human CD8 (MCA351F) and isotype control (MCA928) detected with Goat anti Mouse IgG: RPE (STAR76). Figure B. FITC conjugated Rat anti Human CD8 (MCA351F) and Mouse anti Human CD45RB (MCA1114) detected with Goat anti Mouse IgG RPE (STAR76). All experiments performed on red cell lysed human blood gated on single lymphocytes in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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Goat anti Mouse IgG:RPE (Rat Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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STAR76
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RPE conjugated Goat anti Mouse IfF antibody recognizes both the heavy and light chains of mouse IgG. The reagent has been adsorbed to minimise cross-reactivity with rat immunoglobulins and is therefore of particular value in detecting mouse primary antibodies bound to rat tissues.

We recommend diluting this product in buffer containing 10% normal rat serum to remove any remaining cross-reactivity.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0ml distilled water
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Mouse IgG, whole molecule.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

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References for IgG antibody

  1. Rao, W.H. et al. (2000) Potent costimulation of effector T lymphocytes by human collagen type I.
    J Immunol. 165 (9): 4935-40.
  2. Rópolo, A. et al. (2001) Diminished percentage of antigen bearing cells in the lymph nodes of immune aged rats.
    Exp Gerontol. 36: 519-35.

RRID
AB_323251
UniProt
P01869
P01865
P01867
P01868
P03987
P01864
P01863
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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