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IgG antibody

Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)

Product Type
Polyclonal Antibody
Polyclonal IgG

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P RE WB 1 mg loader
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Rabbit anti Mouse IgG antibody recognizes all subclasses of murine IgG. Some cross reactivity with IgM will be expected.

Cross reactivity is expected with rat IgG. Cross reactivity with human serum has been minimised by solid phase adsorbtion.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Purified IgG framents were prepared by affinity chromatography of serum.

F(ab)2 fragments were prepared by pepsin digestion, followed by gel filtration to remove any intact IgG or Fc fragments.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Purified whole mouse immunoglobulin G.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Immunohistology - Frozen 1/50
Immunohistology - Paraffin 1/50
Immunohistology - Resin 1/50
Western Blotting 1/2000 1/5000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for IgG antibody

  1. Yata, Y. et al. (1999) An improved method for the purification of stellate cells from rat liver with dichloromethylene diphosphate (CL2MDP).
    Methods Cell Sci. 21: 19-24.
  2. Scott, J.L. et al. (2006) Leucocyte population changes in the reproductive tract of the ewe in response to insemination.
    Reprod Fertil Dev. 18: 627-34.
  3. Siepe, M. et al. (2006) Myoblast-seeded biodegradable scaffolds to prevent post-myocardial infarction evolution toward heart failure.
    J Thorac Cardiovasc Surg. 132: 124-31.
  4. Scott, J.L. et al. (2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
    Reprod Fertil Dev. 19: 585-93.
  5. Siepe, M. et al. (2007) Construction of skeletal myoblast-based polyurethane scaffolds for myocardial repair.
    Artif Organs. 31: 425-33.
  6. Johnson, A.E. et al. (2009) AZD2184: a radioligand for sensitive detection of beta-amyloid deposits.
    J Neurochem. 108: 1177-86.
  7. Scott, J.L. et al. (2009) Spermatozoa and seminal plasma induce a greater inflammatory response in the ovine uterus at oestrus than dioestrus.
    Reprod Fertil Dev. 21: 817-26.
  8. Raida, M.K. et al. (2011) Association between plasma antibody response and protection in rainbow trout Oncorhynchus mykiss immersion vaccinated against Yersinia ruckeri.
    PLoS One. 6: e18832.
  9. View The Latest Product References
  10. von Gersdorff Jφrgensen, L. et al. (2011) Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifilii (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum).
    J Fish Dis. 34: 749-55.
  11. Skov, J. et al. (2012) Immunomodulatory effects of dietary β-1,3-glucan from Euglena gracilis in rainbow trout (Oncorhynchus mykiss) immersion vaccinated against Yersinia ruckeri.
    Fish Shellfish Immunol. 33: 111-20.
  12. Buffoni, L. et al. (2012) Humoral immune response in goats immunised with cathepsin L1, peroxiredoxin and Sm14 antigen and experimentally challenged with Fasciola hepatica.
    Vet Parasitol. 185: 315-21.
  13. Emara, M. et al. (2012) Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.
    J Biol Chem. 287: 5756-63.
  14. Chettri, J.K. et al. (2013) Comparative evaluation of administration methods for a vaccine protecting rainbow trout against Yersinia ruckeri O1 biotype 2 infections.
    Vet Immunol Immunopathol. 154: 42-7.
  15. Villumsen, K.R. et al. (2014) Oral and Anal Vaccination Confers Full Protection against Enteric Redmouth Disease (ERM) in Rainbow Trout.
    PLoS One. 9(4):e93845.
  16. Jaafar RM et al. (2015) Effects of adjuvant Montanide™ ISA 763 A VG in rainbow trout injection vaccinated against Yersinia ruckeri.
    Fish Shellfish Immunol. 47 (2): 797-806.
  17. Moradi, B. et al. (2016) Construction of a Novel DNA Vaccine Candidate Encoding an HspX-PPE44-EsxV Fusion Antigen of Mycobacterium tuberculosis.
    Rep Biochem Mol Biol. 4 (2): 89-97.
  18. Marana, M.H. et al. (2017) Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout Oncorhynchus mykiss evaluated by a tail fin infection model.
    J Fish Dis. 40 (4): 507-16.
  19. Pérez-Caballero, R. et al. (2018) Expression of free radicals by peritoneal cells of sheep during the early stages of Fasciola hepatica. infection.
    Parasit Vectors. 11 (1): 500.
  20. Buffoni, L. et al. (2020) Identification of protective peptides of Fasciola hepatica-derived cathepsin L1 (FhCL1) in vaccinated sheep by a linear B-cell epitope mapping approach.
    Parasit Vectors. 13 (1): 390.
  21. Moradi, B. et al. (2020) A new DNA vaccine expressing HspX-PPE44-EsxV fusion antigens of Mycobacterium tuberculosis induced strong immune responses.
    Iran J Basic Med Sci. 23 (7): 909-14.
  22. Zafra, R. et al. (2021) Efficacy of a multivalent vaccine against Fasciola hepatica. infection in sheep.
    Vet Res. 52 (1): 13.
  23. De Vooght, L. et al. (2022) Targeting the tsetse-trypanosome interplay using genetically engineered Sodalis glossinidius..
    PLoS Pathog. 18 (3): e1010376.
  24. Jaafar, R.M. et al. (2018) Secondary immune response of rainbow trout following repeated immersion vaccination.
    J Fish Dis. 41 (1): 117-23.


Western Blotting

Entrez Gene
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis


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