IgG IgA IgM antibody

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Goat anti Mouse IgG/A/M:Alk. Phos.

Goat anti Mouse IgG/A/M:FITC (Human Adsorbed)

Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
STAR87A C E WB 1 mg
STAR87F F 1.8 mg
STAR87P E 1 mg
Goat anti Mouse IgG/A/M (Human Adsorbed) antibody recognizes all subclasses of murine immunoglobulin and has been adsorbed against human, bovine and hamster immunoglobulins.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to alkaline phosphatase - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to mouse immunoglobulins were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Sodium Azide
1%Bovine Serum Albumin
50%Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Immunogen
Mouse IgG purified from normal mouse serum, mouse IgA and IgM purified from ascitic fluid.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.8 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
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GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
Regulatory
For research purposes only

Applications of IgG IgA IgM antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/3000
Immunohistology - Frozen
Western Blotting
Flow Cytometry 1/100 1/500
ELISA 1/1000 1/3000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for IgG IgA IgM antibody

  1. Bergmeier, L.A. et al. (2005) Mucosal alloimmunization elicits T-cell proliferation, CC chemokines, CCR5 antibodies and inhibition of simian immunodeficiency virus infectivity.
    J Gen Virol. 86: 2231-8.
  2. Peters, B. et al. (2004) Effect of heterosexual intercourse on mucosal alloimmunisation and resistance to HIV-1 infection.
    Lancet. 363: 518-24.
  3. Bartlomiejczyk, M.A. et al. (2014) Interaction of lectin pathway of complement-activating pattern recognition molecules with mycobacteria.
    Clin Exp Immunol. 178 (2): 310-9.