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Mouse IgG1 Negative Control antibody

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Mouse IgG1 Negative Control Antibody (Human) thumbnail image 1 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 2 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 3 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 4 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 5 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 6 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 7 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 8 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 9 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 10 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 11 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 12 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 13 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 14 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 15 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 16 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 17 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 18 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 19 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 20 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 21 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 22 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 23 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 24 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 25 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 26 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 27 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 28 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 29 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 30 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 31 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 32 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 33 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 34 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 35 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 36 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 37 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 38 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 39 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 40 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 41 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 42 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 43 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 44 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 45 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 46 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 47 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 48 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 49 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 50 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 51 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 52 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 53 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 54 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 55 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 56 Mouse IgG1 Negative Control Antibody (Human) thumbnail image 57
Mouse IgG1 Negative Control Antibody (Human) gallery image 1
Figure A. RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C) and A488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C) and A488 conjugated Mouse anti Human CD38 (MCA1019A488). All experiments performed on human peripheral blood lymphocytes in the presence of Human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 2
Figure A. A488 conjugated Mouse anti Human CD38 (MCA1019A488) and RPE-A647 tandem conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. A488 conjugated Mouse anti Human CD38 (MCA1019A488) and RPE-Cy5 tandem conjugated Mouse anti Human CD3 (MCA463C). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 3
Figure A. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody (MCA477F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human HLA DP/DQ/DR antibody (MCA477F) and RPE conjugated Mouse anti Human CD11c (MCA2087PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070)
Mouse IgG1 Negative Control Antibody (Human) gallery image 4
Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse anti Human CD18 (MCA2086PE). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070).
Mouse IgG1 Negative Control Antibody (Human) gallery image 5
Figure A. RPE conjugated Mouse anti Human CD18 (MCA2086PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Human CD18 (MCA2086PE) and FITC conjugated Mouse anti Human CD3 (MCA463F). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 6
Figure A. RPE conjugated Rat anti Human CD28 (MCA709PE) and A647 conjugated Mouse IgG1 istoype control (MCA928A647). Figure B. RPE conjugated Rat anti Human CD28 (MCA709PE) and A647 conjugated Mouse anti Human CD4 (MCA1267A647). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 7
Figure A. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. APC conjugated Mouse anti Human CD3 (MCA463APC) and RPE conjugated Mouse anti Human CD46 (MCA2113PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 8
Figure A. RPE conjugated Mouse anti Human CD46 (MCA2113PE) and APC conjugated Mouse IgG1 isotype control (MCA928APC). Figure B. RPE conjugated Mouse anti Human CD46 (MCA2113PE) and APC conjugated Mouse anti Human CD3 (MCA463APC). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 9
Figure A. RPE conjugated Rat anti Human CD49f (MCA699PE) and A488 conjugated Mouse IgG1 control (MCA928PE). Figure B. RPE conjugated Rat anti Human CD49f (MCA699PE) and A488 conjugated Mouse anti Human CD3 (MCA463A488). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 10
Figure A. RPE conjugated Mouse anti Human CD59 (MCA1054PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Human CD59 (MCA1054PE) and FITC conjugated Mouse anti Human CD3 (MCA463F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 11
Image A. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse IgG1 isotype control (MCA928F). Image B. FITC conjugated Mouse anti Human CD66b (MCA216F) and RPE conjugated Mouse anti Human CD177 (MCA2045PE). All experiments performed on human Peripheral blood derived myeloid cells in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 12
Figure A. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647) and FITC conjugated Mouse anti Human CD53 (MCA723F). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 13
Figure A. FITC conjugated Mouse anti Human CD53 (MCA723F) and RPE-A647 tandem conjugated Mouse IgG1 isotype control (MCA928P647). Figure B. FITC conjugated Mouse anti Human CD53 (MCA723F) and RPE-A647 tandem conjugated Mouse anti Human CD3 (MCA463P647). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 14
Figure A. A700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. A700 conjugated Mouse anti Human CD3 (MCA463A700) and RPE conjugated Mouse anti Human CD55 (MCA1614PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 15
Figure A. PE conjugated Mouse anti CD55 (MCA1614PE) and A700 conjugated Mouse IgG1 isotype control (MCA928A700). Figure B. PE conjugated Mouse anti CD55 (MCA1614PE) and A700 conjugated Mouse anti Human CD3 (MCA463A700). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).
Mouse IgG1 Negative Control Antibody (Human) gallery image 16
Figure A. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse anti Porcine CD8a (MCA1223PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 17
Figure A. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse anti Porcine CD8b (MCA5954PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 18
Figure A. FITC conjugated Mouse anti Porcine CD52 (MCA5974F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD52 (MCA5974F) and RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 19
Figure A. RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE) and FITC conjugated Mouse IgG21 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Porcine CD45RA (MCA1751PE) and FITC conjugated Mouse anti Porcine CD52 (MCA5974F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 20
Figure A. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD4a (MCA1749F) and purified Mouse anti Porcine CD25 (MCA1736GA) detected with Goat anti Mouse IgG1PE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 21
Figure A. Purified Mouse anti Porcine CD45RC (MCA1750) detected with Goat anti Mouse IgM PE (STAR86PE) and Mouse IgG1 FITC isotype control (MCA928F). Figure B. Purified Mouse anti Porcine CD45RC (MCA1750) detected with Goat anti Mouse IgM PE (STAR86PE) and Mouse anti Porcine CD3 FITC (MCA5951F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 22
Figure A. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse anti Porcine CD11R1 (MCA1220) detected with Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 23
Figure A. FITC conjugated Mouse anti Porcine CD18a (MCA1972F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD18a (MCA1972F) and RPE conjugated Mouse anti Porcine CD27 (MCA5973PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 24
Figure A. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated Mouse anti Porcine CD11a (MCA2308F) and purified Mouse anti Porcine CD46 (MCA2262) detected with Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 25
Figure A. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and A647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Porcine CD14 (MCA1218F) and A647 conjugated Mouse anti Porcine CD45 (MCA1222A647). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 26
Figure A. FITC conjugated Mouse anti Porcine CD11R3 (MCA2309F) and APC conjugated Mouse IgG1 isotype control (MCA928APC). Figure B. FITC conjugated Mouse anti Porcine CD11R3 (MCA2309F) and APC conjugated Mouse anti Porcine CD31. All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 27
Figure A. APC conjugated Mouse anti Porcine CD31 (MCA1746APC) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. APC conjugated Mouse anti Porcine CD31 (MCA1746APC) and FITC conjugated Mouse anti Porcine CD11R3 (MCA2309F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 28
Figure A. FITC conjugated Mouse anti Porcine CD45 (MCA1222F) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD45 (MCA1222F) and PE conjugated Mouse anti Porcine CD16 (MCA1971PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 29
Figure A. PE conjugated Mouse anti Porcine CD16 (MCA1971PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Porcine CD16 (MCA1971PE) and FITC conjugated Mouse anti Porcine CD45 (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 30
Figure A. A647 conjugated Mouse anti Porcine CD45 (MCA1222A647) and A488 conjugated Mouse IgG1 isotype control (MCA928A488). Figure B. A647 conjugated Mouse anti Porcine CD45 (MCA1222A647) and A488 conjugated Mouse anti Porcine CD117 (MCA2598A488). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 31
Figure A. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and PE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Porcine CD45RA (MCA1751F) and PE conjugated Mouse anti Porcine CD163 (MCA2311PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 32
Figure A. PE conjugated Mouse anti Porcine CD163 (MCA2311PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Porcine CD163 (MCA2311PE) and FITC conjugated Mouse anti Porcine CD45RA (MCA1751F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 33
Figure A. RPE conjugated Rat anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Rat anti Canine CD4 (MCA1038PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 34
Figure A. RPE conjugated Rat anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Rat anti Canine CD8 (MCA1039PE) and FITC conjugated Mouse anti Canine CD3 (MCA1774F). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 35
Figure A. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. A647 conjugated Mouse anti Canine CD18 (MCA1780A647) and RPE conjugated Mouse anti Canine CD21 (MCA1781PE). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 36
Figure A. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. RPE conjugated Mouse anti Canine CD21 (MCA1781PE) and A647 conjugated Mouse anti Canine CD18 (MCA1780A647). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 37
Figure A. FITC conjugated Rat anti Canine CD45 (MCA1042F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Rat anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11b (MCA1777S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 38
Figure A. PE conjugated Rat anti Canine CD45 (MCA1042PE) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Rat anti Canine CD45 (MCA1042F) and purified Mouse anti Canine CD11c (MCA1778S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on monocytes.
Mouse IgG1 Negative Control Antibody (Human) gallery image 39
Figure A. A488 conjugated Rat anti Canine CD4 (MCA1038A488) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. A488 conjugated Rat anti Canine CD4 (MCA1038A488) and purified Mouse anti Canine CD8b (MCA1775S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 40
Figure A. FITC conjugated Rat anti Canine CD8 (MCA1039F) and purified Mouse IgG1 isotype control (MCA928) detected with Goat anti Mouse IgG1 DyLight 649. Figure B. FITC conjugated Rat anti Canine CD8 (MCA1039F) and purified Mouse anti Canine CD45RA (MCA2036S) detected with Goat anti Mouse IgG1 DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 41
Figure A. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse anti Bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells
Mouse IgG1 Negative Control Antibody (Human) gallery image 42
Figure A. RPE conjugated Mouse anti Bovine CD8 (MCA837PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA837PE) and FITC conjugated Mouse anti Bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 43
Figure A. Purified Mouse anti Bovine CD1w3 (MCA1647G) detected with Goat anti Mouse IgG2b RPE (STAR134PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. Purified Mouse anti Bovine CD1w3 (MCA1647G) detected with Goat anti Mouse IgG2b RPE (STAR134PE) and FITC conjugated Mouse anti Bovine CD40 (MCA2431F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 44
Figure A. FITC conjugated Mouse anti Bovine CD21 (MCA5953F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD21 (MCA5953F) and RPE conjugated Mouse anti Bovine CD8b (MCA1654PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 45
Figure A. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and purified Mouse IgG1 isotype control (MCA928) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). Figure B. FITC conjugated Mouse anti Bovine CD4 (MCA1653F) and purified CD5 (MCA835GA) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 46
Figure A. RPE conjugated Mouse anti Bovine CD28 (MCA5779PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD28 (MCA5779PE) and FITC conjugated Mouse anti Bovine CD44 (MCA2433F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 47
Figure A. FITC conjugated Mouse anti Bovine CD44 (MCA2433F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD44 (MCA2433F) and RPE conjugated Mouse anti Bovine CD8b (MCA5779PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 48
Figure A. FITC conjugated Mouse anti bovine CD62L (MCA1649F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD62L (MCA1649F) and RPE conjugated Mouse anti Bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 49
Figure A. RPE conjugated Mouse anti Bovine CD80 (MCA2436PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD80 (MCA2436PE) and FITC conjugated Mouse anti Bovine CD62L (MCA1649F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 50
Figure A. FITC conjugated Mouse anti Bovine CD8 (MCA837F) and A647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Bovine CD8 (MCA837F) and A647 conjugated Mouse anti Bovine CD26 (MCA1652A647). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 51
Figure A. FITC conjugated Mouse anti Bovine CD14 (MCA2678F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti bovine CD14 (MCA2678F) and RPE conjugated Mouse anti Bovine CD80 (MCA2436PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 52
Figure A. FITC conjugated Mouse anti Bovine CD11b (MCA1425F) and purified Mouse IgG1 isotype control (MCA928) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). Figure B. FITC conjugated Mouse anti Bovine CD11b (MCA1425F) and purified CD13 (MCA2338GA) detected with RPE conjugated Goat anti Mouse IgG1 (STAR132PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 53
Figure A. FITC conjugated Mouse anti Bovine CD32 (MCA5919F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD32 (MCA5919F) and RPE conjugated Mouse anti Bovine CD205 (MCA2450PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 54
Figure A. FITC conjugated Mouse anti Bovine CD282 (HCA151F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD282 (HCA151F) and RPE conjugated Mouse anti Bovine CD32 (MCA5918PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 55
Figure A. RPE conjugated Mouse anti Bovine CD335 (MCA2365PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD335 (MCA2365PE) and FITC conjugated Mouse anti Bovine CD2 (MCA833F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 56
Figure A. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse anti Bovine CD335 (MCA2365PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Mouse IgG1 Negative Control Antibody (Human) gallery image 57
Figure A. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. RPE conjugated Mouse anti Bovine CD4 (MCA1653PE) and FITC conjugated Mouse anti Bovine CD25 (MCA2430F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Mouse IgG1 Negative Control:RPE

Product Type
Negative/Isotype Control
Isotype
IgG1
Specificity
Mouse IgG1 Negative Control

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Mouse IgG1 negative control is negative by flow cytometry on all human cells and cell lines tested. Further tests have also shown that this reagent is also suitable for use as a negative control with bovine (Maslanka et al, 2012), ovine, porcine (Kapetanovic et al, 2012), equine (Jacks et al, 2007), canine (Maiolini et al, 2012) , lapine (Pakandl et al, 2008) and guinea-pig tissues.

This reagent recognizes a rat cell surface marker, and therefore cannot be used as a negative control in this species.

Target Species
Negative Control
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry *
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. *It is recomended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 separated cells or 100ul lysed whole peripheral blood.

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References for Mouse IgG1 Negative Control antibody

  1. Kupatt, C. et al. (2000) c7E3Fab reduces postischemic leukocyte-thrombocyte interaction mediated by fibrinogen. Implications for myocardial reperfusion injury.
    Arterioscler Thromb Vasc Biol. 20 (10): 2226-32.
  2. Dalli, J. et al. (2008) Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles.
    Blood. 112 (6): 2512-9.
  3. Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
    J Immunol. 187: 4913-9.
  4. Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
    J Immunol. 188: 3382-94.
  5. Maiolini, A. et al. (2012) Toll-like receptors 4 and 9 are responsible for the maintenance of the inflammatory reaction in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis.
    J Neuroinflammation. 9: 226.
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    9. View The Latest Product References
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RRID
AB_322263

MCA928PE

1608

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