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CD4 Alpha antibody | MIL17

Mouse anti Pig CD4 Alpha:FITC

Product Type
Monoclonal Antibody
CD4 Alpha

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price

Mouse anti Porcine CD4 alpha, clone MIL17 recognizes a ~55 kDa porcine homologue to the human CD4 antigen found on the surface of helper-T cells. MIL-17 was confirmed as a member of the CD4 alpha cluster at the ‘Third International Workshop on Swine Leukocyte Differentiation Antigens’ (Haverson et al. 2001). Porcine CD4 is a type 1 trans-membrane member of the immunoglobulin superfamily.

Pigs appear unusual amongst mammalian species as they appear to have four populations of resting T lymphocytes. In addition to the two populations of mutually exclusive CD4+/CD8- and CD4-/CD8+ lymphocytes, they also appear to have significant populations of CD4-/CD8- and CD4+/CD8+ cells. Lymphoblasts with a double positive phenotype have been described in other species but this is not the case for mature T lymphocytic calls (Saalmuller et al. 1987)

Mouse anti Pig CD4 alpha, clone MIL17 stains a population of cells with characteristic lymphocyte morphology in immunohistochemistry (Inman et al. 2010).

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Leucocytes isolated from porcine gut lamina propria.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader
List Price Your Price
Description Mouse IgG2b Negative Control:FITC

References for CD4 Alpha antibody

  1. Saalmüller A et al. (2001) Summary of workshop findings for porcine T-lymphocyte-specific monoclonal antibodies.
    Vet Immunol Immunopathol. 80 (1-2): 35-52.
  2. Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage.
    Am J Pathol. 176: 1648-59.
  3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
    J Leukoc Biol. 88 (3): 571-8.
  4. Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
    Res Vet Sci. 91 (3): e68-72.
  5. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs.
    J Anim Sci. 90: 649-56.
  6. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys.
    Kidney Int. 58: 838-50.
  7. Tambuyzer BR et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro.
    Cell Biol Int. 36 (12): 1233-8.
  8. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs.
    BMC Vet Res. 8: 232.
  9. View The Latest Product References
  10. Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets.
    Vaccine. 28: 6006-13.
  11. Clapperton, M. et al. (2005) Associations of weight gain and food intake with leukocyte sub-sets in Large White pigs
    Livestock Production Science 96: 249-60
  12. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs.
    Vet Immunol Immunopathol. 104: 131-44.
  13. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance.
    Animal. 2: 1575-84.
  14. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia-reperfusion and renal medulla injury.
    J Pharmacol Exp Ther. 2002 Sep;302(3):861-70.
  15. Faure, J.P. et al. (2004) Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model.
    Nephrol Dial Transplant. 19: 1742-51.
  16. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
    PLoS One. 7(3): e33707.
  17. Langerhuus, S.N. et al. (2010) Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus.
    Eur J Clin Microbiol Infect Dis. 29: 1453-6.
  18. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine.
    BMC Genomics. 13: 488.
  19. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs.
    J Swine Health Prod 20: 276-282.
  20. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2.
    Vet Microbiol. 129: 367-77.
  21. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning.
    J Nutr. 131: 1520-7.
  22. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining.
    J Immunol Methods. 327: 18-29.
  23. Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes.
    PLoS One. 7(3): e32400.
  24. Akershoek, J.J. et al. (2016) Cell therapy for full-thickness wounds: are fetal dermal cells a potential source?
    Cell Tissue Res. 364 (1): 83-94.
  25. Liu J et al. (2016) The Role of Porcine Monocyte Derived Dendritic Cells (MoDC) in the Inflammation Storm Caused by Streptococcus suis Serotype 2 Infection.
    PLoS One. 11 (3): e0151256.
  26. Liermann, W. et al. (2017) Effects of two commercial diets and technical feed treatment on stomach lesions and immune system of fattening pigs.
    J Anim Physiol Anim Nutr (Berl). 101 (5): e414-26.
  27. Gardner, D.S. et al. (2016) Remote effects of acute kidney injury in a porcine model.
    Am J Physiol Renal Physiol. 310 (4): F259-71.
  28. Hemmink, J.D. et al. (2016) Distinct immune responses and virus shedding in pigs following aerosol, intra-nasal and contact infection with pandemic swine influenza A virus, A(H1N1)09.
    Vet Res. 47 (1): 103.
  29. Dąbrowski, M. et al. (2017) The Effect of Deoxynivalenol on Selected Populations of Immunocompetent Cells in Porcine Blood-A Preliminary Study.
    Molecules. 22 (5): 691.
  30. Hsu, W.T. et al. (2013) Prostaglandin E2 potentiates mesenchymal stem cell-induced IL-10+IFN-γ+CD4+ regulatory T cells to control transplant arteriosclerosis.
    J Immunol. 190 (5): 2372-80.
  31. Matsubara, T. et al. (2015) Identification of a CD4 variant in Microminipigs not detectable with available anti-CD4 monoclonal antibodies.
    Vet Immunol Immunopathol. 168 (3-4): 176-83.
  32. Hu, Z. et al. (2019) Genomic variant in porcine TNFRSF1A gene and its effects on TNF signaling pathway in vitro.
    Gene. 700: 105-9.
  33. Fogle, J.E. et al. (2019) Antibiotic Therapy Does Not Alter the Humoral Response to Vaccination for Porcine Circovirus 2 in Weaned Pigs.
    Vet Sci. 6(2): 51.
  34. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
  35. Christoforidou, Z. et al. (2019) Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets.
    Front Immunol. 10: 2705.
  36. López, E. et al. (2019) Identification of very early inflammatory markers in a porcine myocardial infarction model.
    BMC Vet Res. 15 (1): 91.
  37. Liu, K.Y. et al. (2021) Fallopian tube stem cell medium of porcine and bovine: In vitro. regenerative effect on maturation and parthenogenesis of porcine oocytes.
    Res Vet Sci. 140: 83-90.
  38. Giese, I.M. et al. (2020) Chronic Hyperglycemia Drives Functional Impairment of Lymphocytes in Diabetic INS C94Y Transgenic Pigs.
    Front Immunol. 11: 607473.
  39. Nielsen, O.L. et al. (2022) A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study.
    APMIS. 130 (7): 359-70.
  40. Maciag, S.S. et al. (2022) On the influence of the source of porcine colostrum in the development of early immune ontogeny in piglets.
    Sci Rep. 12 (1): 15630.
  41. Melgoza-González, A.E. et al. (2022) Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells
    Vaccines. 10 (5): 684.
  42. Zhou, L. et al. (2022) Clinical improvement of sepsis by extracorporeal centrifugal leukocyte apheresis in a porcine model.
    J Transl Med. 20 (1): 538.
  43. Wu, M.C. et al. (2023) A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs.
    Vet Res. 54 (1): 17.
  44. Bujňák, L. et al. (2023) The Effect of Dietary Humic Substances on Cellular Immunity and Blood Characteristics in Piglets
    Agriculture. 13 (3): 636.
  45. Schilloks, M.C. et al. (2023) Effects of GHR Deficiency and Juvenile Hypoglycemia on Immune Cells of a Porcine Model for Laron Syndrome.
    Biomolecules. 13 (4): 597.
  46. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Flow Cytometry


Immunohistology - Frozen



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