Mouse IgG2a Negative Control antibody

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Mouse IgG2a Negative Control Antibody - Rat gallery image 1 — Figure A. FITC conjugated Mouse anti Human CD31 (MCA1738F) and A647 conjugated Mouse IgG2a isotype control (MCA929A647). Figure B. FITC conjugated Mouse anti Human CD31 (MCA1738F) and A647 conjugated Mouse CD14 (MCA1568A647). All experiments performed on human peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

Mouse IgG2a Negative Control Antibody - Rat gallery image 2 — Figure A. RPE conjugated Mouse anti Human CD9 (MCA469PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Human CD9 (MCA469PE) and FITC conjugated Mouse anti Human CD14 (MCA596F). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

Mouse IgG2a Negative Control Antibody - Rat gallery image 3 — Figure A. RPE conjugated Mouse anti Human CD11c (MCA2087PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Human CD11c (MCA2087PE) and FITC conjugated Mouse anti Human HLA DP/DQ/DR (MCA477F). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

Mouse IgG2a Negative Control Antibody - Rat gallery image 4 — Figure A. FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse IgG2a isotype control (MCA929A647). Figure B FITC conjugated Mouse anti Human CD19 (MCA1940F) and A647 conjugated Mouse anti Human CD27 (MCA755A647). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG2a Negative Control Antibody - Rat gallery image 5 — Figure A. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Human CD3 (MCA463F) and RPE conjugated Mouse anti Human CD59 (MCA1054PE). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

Mouse IgG2a Negative Control Antibody - Rat gallery image 6 — Figure A. FITC conjugated Mouse anti Human CD86 (MCA1118F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Human CD86 (MCA1118F) and RPE conjugated Mouse anti Human HLA DP/DQ/DR (MCA477PE). All experiments performed on human Peripheral blood mononuclear cells in the presence of human SeroBlock (BUF070A).

Mouse IgG2a Negative Control Antibody - Rat gallery image 7 — Figure A. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse IgG2a isotype control (MCA929PE). Figure B. FITC conjugated Mouse anti Porcine CD5 (MCA2307F) and RPE conjugated Mouse anti Porcine CD4a (MCA1749PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells

Mouse IgG2a Negative Control Antibody - Rat gallery image 8 — Figure A. RPE conjugated Mouse anti Porcine CD8a (MCA1223PE) and FITC conjugated Mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Porcine CD8a (MCA1223PE) and FITC conjugated Mouse anti Porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 9 — Figure A. RPE conjugated Mouse anti Porcine CD8b (MCA5954PE) and FITC conjugated mouse IgG2a isotype control (MCA929F). Figure B. RPE conjugated Mouse anti Porcine CD8b (MCA5954PE) and FITC conjugated Mouse anti Porcine CD5 (MCA2307F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 10 — Figure A. FITC conjugated Mouse anti Porcine CD45 (MCA1222F) and purified Mouse IgG2a isotype control (MCA929) detected with Goat anti Mouse IgG2a PE (STAR133PE). Figure B. FITC conjugated Mouse anti Porcine CD45 (MCA1222F) and purified Mouse anti Porcine CD6 (MCA1221GA) detected with Goat anti Mouse IgG2a (STAR133PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 11 — Figure A. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse IgG2a isotype control (**MCA929PE**). Figure B. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse anti Bovine CD4 (MCA1653PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 12 — Figure A. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse IgG2a isotype control (**MCA929PE**). Figure B. FITC conjugated Mouse anti Bovine CD2 (MCA833F) and RPE conjugated Mouse anti Bovine CD8 (MCA837PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 13 — Figure A. Purified Mouse anti Bovine CD5 (MCA835GA) detected with Goat anti Mouse IgG1 RPE (STAR132PE) and FITC conjugated Mouse IgG2a isotype control (**MCA929F**). Figure B. Purified Mouse anti Bovine CD5 (MCA835GA) detected with Goat anti Mouse IgG1 RPE (STAR132PE) and FITC conjugated Mouse anti Bovine CD4 (MCA1653F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 14 — Figure A. A647 conjugated Mouse anti Bovine CD26 (MCA1652A647) and FITC conjugated Mouse IgG2a isotype control (**MCA929F**). Figure B. A647 conjugated Mouse anti Bovine CD26 (MCA1652A647) and FITC conjugated Mouse anti Bovine CD8 (MCA837F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 15 — Figure A. RPE conjugated Mouse anti Bovine CD205 (MCA2450PE) and FITC conjugated Mouse IgG2a isotype control (**MCA929F**). Figure B. RPE conjugated Mouse anti Bovine CD205 (MCA2450PE) and FITC conjugated Mouse anti Bovine CD32 (MCA5919F). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

Mouse IgG2a Negative Control Antibody - Rat gallery image 16 — Figure A. FITC conjugated Mouse anti Bovine CD25 (MCA2430F) and RPE conjugated Mouse IgG2a isotype control (**MCA929PE**). Figure B. FITC conjugated Mouse anti Bovine CD25 (MCA2430F) and RPE conjugated Mouse anti Bovine CD4 (MCA1653PE). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.

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Mouse IgG2a Negative Control:FITC

Product Type: Negative/Isotype Control
Isotype: IgG2a
Specificity: Mouse IgG2a Negative Control

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References (3)
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Mouse IgG2a negative control antibody, clone OX-34 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue.

Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species.

This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control.

Test results have shown that MCA929 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.

This antibody may not be suitable for intracellular staining on some cell types.

Target Species: Negative Control
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Immunogen: Activated rat T-helper cells.
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			*

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures.
* It is recommended that the user dilutes the antibody for use in their own system to a concentration equivalent to their test reagent.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

References for Mouse IgG2a Negative Control antibody

Avigdor, A. et al. (2004) CD44 and hyaluronic acid cooperate with SDF-1 in the trafficking of human CD34+ stem/progenitor cells to bone marrow.
Blood. 103 (8): 2981-9.
Kamble, N.M. et al. (2016) Interaction of a live attenuated Salmonella gallinarum vaccine candidate with chicken bone marrow-derived dendritic cells.
Avian Pathol. Jan 26:1-24. [Epub ahead of print]
Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
Vet Res. 48 (1): 20.